Nuclease-Resistant DNA via High-Density Packing in Polymeric Micellar Nanoparticle Coronas
Herein, we describe a polymeric micellar nanoparticle capable of rendering nucleic acids resistant to nuclease digestion. This approach relies on utilizing DNA as the polar headgroup of a DNA-polymer amphiphile in order to assemble well-defined, discrete nanoparticles. Dense packing of DNA in the micelle corona allows for hybridization of complementary oligonucleotides while prohibiting enzymatic degradation. We demonstrate the preparation, purification, and characterization of the nanoparticles, then describe their resistance to treatment with endo- and exonucleases including snake-venom phosphodiesterase (SVP), a common, general DNA digestion enzyme.
[Show abstract] [Hide abstract] ABSTRACT: A new tricarbocyanine-based chemosensor exhibited a dramatic Al(3+)-specific fluorescence turn-on response in the near-infrared (NIR) region. The receptor was found to be highly selective towards Al(3+) over other metal ions in physiological condition. The sensor was non-toxic and could thus be employed as an imaging probe for detecting intracellular Al(3+) in live cells. Interestingly, upon interaction with DNA in solution, the L-Al(3+) ensemble rendered tracking of DNase activity in solution through a systematic reduction in the fluorescence emission intensity. Copyright © 2015 Elsevier B.V. All rights reserved.0Comments 1Citation
- "Nucleases are considered to be vital in various biological processes involving DNA replication, recombination , DNA repair, genotyping, and mapping analysis. Literature reports also highlight the merit of developing sensing platforms for nuclease assay434445. In order to pursue this goal, L–Al 3+ complex was interacted in solution with calf thymus DNA (CT-DNA). "
- [Show abstract] [Hide abstract] ABSTRACT: Materials nanoarchitectonics has led to important innovations in the design and construction of systems in nanoelectronics, nanomachinery, and energy conversion. Recent publications point to the fact that the same approach may be applied successfully to other fields. In this perspective, we define the key features of materials nanoarchitectonics and examine how they can be used to address current challenges in nanomedicine, placing emphasis on colloidal agents for therapeutic and diagnostic applications.0Comments 12Citations
- [Show abstract] [Hide abstract] ABSTRACT: In this review, we survey the diversity of structures and functions which are encountered in advanced self-assembled nanomaterials. We highlight their flourishing implementations in three active domains of applications: biomedical sciences, information technologies, and environmental sciences. Our main objective is to provide the reader with a concise and straightforward entry to this broad field by selecting the most recent and important research articles, supported by some more comprehensive reviews to introduce each topic. Overall, this compilation illustrates how, based on the rules of supramolecular chemistry, the bottom-up approach to design functional objects at the nanoscale is currently producing highly sophisticated materials oriented towards a growing number of applications with high societal impact.0Comments 127Citations