The 5 x 107 Jurkat cells were stimulated by 50 ng/mL IL-1 for 10 min, collected, lysed, resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with IRAK4 (phospho T345) monoclonal antibody, clone A8A8 (Cat # MAB2538) at 1 : 500 (Lane 1). Lane 2 was pre-incubated with Phosphospecific peptide. Lane 3 was pre-incubate with non-phospho specific peptide.An immunoreactive band is observed around ~51 KDa.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane. A : IRAK4 (pT345). B : IRAK4 (non phosphorylated). Followed by IRAK4 (phospho T345) monoclonal antibody, clone A8A8 (Cat # MAB2538) incubation at a 1 : 1000 dilution.
This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [supplied by RefSeq
IRAK-4 mutated form 1,interleukin-1 receptor associated kinase 4