|Gene:||ynfE||Accession Numbers: G6845 (MetaCyc), b1587, ECK1582|
Species: Escherichia coli K-12 substr. MG1655
Component of: putative selenate reductase (summary available)
YnfE has been implicated as a Tat-dependent selenate reductase enzyme in E. coli. A ynfEF double null mutant is unable to reduce selenate to elemental selenium [Guymer09]. The disruption is specific to the initial selenate reduction process since selenium production is restored when selenite is added to the growth medium [Guymer09]. Production of either YnfE or YnfF from a plasmid restored the ability of the E. coli ynfEF double mutant to reduce selenate to selenium in vivo [Guymer09].
YnfE is highly similar to DmsA, the catalytic subunit of the dimethyl sulfoxide reductase heterotrimer, and cross-reacts with an anti-DmsA antibody. The protein is poorly expressed. In a plasmid expression system, expression of YnfE appears to inhibit expression of YnfFGH [Lubitz03].
In a ΔtusA strain, expression of ynfE is decreased in mid-exponential phase and under aerobic conditions [Dahl13a].
Locations: periplasm, inner membrane
|Map Position: [1,656,093 -> 1,658,519]|
Molecular Weight of Polypeptide: 89.78 kD (from nucleotide sequence)
Unification Links: ASAP:ABE-0005302, DIP:DIP-12765N, EchoBASE:EB3604, EcoGene:EG13843, EcoliWiki:b1587, ModBase:P77374, OU-Microarray:b1587, PortEco:ynfE, Pride:P77374, Protein Model Portal:P77374, RefSeq:NP_416104, RegulonDB:G6845, SMR:P77374, String:511145.b1587, UniProt:P77374
Relationship Links: InterPro:IN-FAMILY:IPR006311, InterPro:IN-FAMILY:IPR006655, InterPro:IN-FAMILY:IPR006656, InterPro:IN-FAMILY:IPR006657, InterPro:IN-FAMILY:IPR006963, InterPro:IN-FAMILY:IPR009010, InterPro:IN-FAMILY:IPR011888, Pfam:IN-FAMILY:PF00384, Pfam:IN-FAMILY:PF01568, Pfam:IN-FAMILY:PF04879, Prosite:IN-FAMILY:PS00932, Prosite:IN-FAMILY:PS51318, Prosite:IN-FAMILY:PS51669, Smart:IN-FAMILY:SM00926
|MultiFun Terms:||metabolism → metabolism of other compounds|
Subunit of: putative selenate reductase
Synonyms: YnfFGH, YnfEFGH
Species: Escherichia coli K-12 substr. MG1655
Subunit composition of
putative selenate reductase = [YnfE][YnfF][YnfG][YnfH]
oxidoreductase subunit = YnfE (summary available)
oxidoreductase subunit = YnfF (extended summary available)
oxidoreductase, predicted Fe-S subunit = YnfG (summary available)
oxidoreductase, predicted membrane anchor subunit = YnfH (summary available)
On the basis of sequence similarity the ynfEFGH operon was predicted to encode an oxidoreductase complex closely related to DMSO reductase. A strain carrying a deletion of dmsABC and containing ynfFGH on a multicopy plasmid is able to grow poorly under anaerobic conditions utilizing dimethyl sulfoxide as a terminal oxidant [Lubitz03]. More recently, genetic analysis of E.coli ynfE and ynfF null mutants suggests these proteins are Tat-targeted selenate reductases [Guymer09]. E.coli ubiE and menA null mutants are unable to reduce selenate to elemental red selenium in vivo thus implicating menaquinone in the reductase activity [Guymer09].
Enzymatic reaction of: selenate reductase
EC Number: 184.108.40.206selenate + an reduced unknown electron acceptor → selenite + an oxidized unknown electron acceptor + H2O
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction of enzyme catalysis.
The reaction is physiologically favored in the direction shown.
|Signal-Sequence||1 -> 43|
|Chain||44 -> 808|
|Conserved-Region||49 -> 110|
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.
Dahl13a: Dahl JU, Radon C, Buhning M, Nimtz M, Leichert LI, Denis Y, Jourlin-Castelli C, Iobbi-Nivol C, Mejean V, Leimkuhler S (2013). "The sulfur carrier protein TusA has a pleiotropic role in Escherichia coli that also affects molybdenum cofactor biosynthesis." J Biol Chem 288(8);5426-42. PMID: 23281480
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Guymer09: Guymer D, Maillard J, Sargent F (2009). "A genetic analysis of in vivo selenate reduction by Salmonella enterica serovar Typhimurium LT2 and Escherichia coli K12." Arch Microbiol 191(6);519-28. PMID: 19415239
Lubitz03: Lubitz SP, Weiner JH (2003). "The Escherichia coli ynfEFGHI operon encodes polypeptides which are paralogues of dimethyl sulfoxide reductase (DmsABC)." Arch Biochem Biophys 418(2);205-16. PMID: 14522592
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