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BBOX1 UltraMAB Antibody, Clone Clone UMAB119
UltraMAB Antibodies - Validated with 10K Protein Chip
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Also for BBOX1 (NM_003986)
|Full length human recombinant protein of human BBOX1 (NP_003977) produced in HEK293T cell.|
|Clone Clone UMAB119
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, 10K-CHIP
|| IHC 1:100~200,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (BBOX1)|
|BBH; BBOX; G-BBH; gamma-BBH|
|This gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. [provided by RefSeq, Jul 2008]. |
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Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.
Immunohistochemical staining of paraffin-embedded human kidney carcinoma using anti-BBOX1 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM500084 requires HIER with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The composit image shows strong cytoplasmic, membrane, and nuclear in panel A tumor cells and no staining in panel B tumor cells but strong staining is normal tubule epithelial cells..
Immunohistochemical staining of paraffin-embedded human breast duct using anti-BBOX1 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM500084 requires HIER with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong cytoplasmic, membrane, and nuclear staining in ductal epithelial cells.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-BBOX1 mouse monoclonal antibody. (UM500084; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescent staining of HeLa cells using anti-BBOX1 mouse monoclonal antibody (UM500084, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-BBOX1 mouse monoclonal antibody (UM500084). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.