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The two‐state theory of protein denaturation, in which it is assumed that a protein exists as two species, native and denatured, is examined by three approaches in this paper. First, the point is made that denaturation of an isolated molecule involves a continuous shift in the probabilities of...
It is shown how the technique of fluorescence may be used to assess conclusions about conformation which are based on a statistical mechanical treatment of simple protein models. Specifically, the state of bonding and distance between two chromophores in a molecule depend on the overall...
A study of the elastic properties of collagen tendons while in the crystalline state while undergoing shrinkage, and while in the amorphous state was carried out. An additional analysis of x‐ray diffraction patterns and birefringence was also performed. Our main observations are the following....
The ability of periodate‐oxidized amylose to form aggregates in aqueous solution was studied by sedimentation, light scattering, and viscosity analyses. Ultracentrifuge schlieren patterns show that aggregation can be appreciable at pH 3.0 and 1.0. The hydroxyl ion‐catalyzed degradation of the...
The effect of salt type and concentration on the transformation of an oriented crystalline collagen tendon into a crosslinked network under conditions of equilibrium swelling was investigated. Our main observations are the following. The degree of swelling of crystalline tendons increases at low...
Calorimetric measurements of the heat of the addition of the second strand of poly U to poly (A + 2U) to form the three‐stranded poly (A + 2U) complex in 0.1M and 0.5M NaCl at 24 and 37°C. are reported. A value of ΔH = −3800 cal./mole of poly (A + 2U) formed was found to be fairly...
Three types of band‐forming centerpieces useful in band centrifugation in the analytical ultracentrifuge are described. The mode of filling and the advantages and disadvantages of each type are discussed.
The partition function of paper II for short polypetide chains is incorporated here into one for a simple model for side‐chain hydrogen bonds between helices. It is thus possible to calculate the fraction of amide hydrogen bonds, the fraction of side‐chain hydrogen bonds, and the number of...
Intrinsic viscosity–temperature studies for isoelectric gelatin in KCl and KSCN aqueous solutions and equilibrium degree of swelling, ν−1, measurements for amorphous crosslinked rat tail tendons in the same solutions were carried out. On increasing salt concentration Cs, both (η) and ν−1...
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