The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 81 kDa.
1/50 - 1/100.
RelevanceProtein Kinase C (PKC) isoforms are serine/threonine kinases involved in signal transduction pathways that govern a wide range of physiological processes including differentiation, proliferation, gene expression, brain function, membrane transport and the organization of cytoskeletal and extracellular matrix proteins. Increasing evidence from studies using in vitro and in vivo systems points to PKC as a key regulator of critical cell cycle transitions, including cell cycle entry and exit and the G1 and G2 checkpoints. PKC-mediated control of these transitions can be negative or positive, depending on the timing of PKC activation during the cell cycle and on the specific PKC isozymes involved. There have been at least 12 different PKC isoforms identified in humans to date, including alpha, beta I, beta II, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, and mu.
Cellular localizationCytoplasmic and membrane associated
ab59363, at a 1/50 dilution, staining Human PKC in breast carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue, minus immunising peptide (left image) and plus immunising peptide (right image).
References for Anti-PKC antibody (ab59363)
This product has been referenced in:
Leiria LO et al. Role of PKC and CaV1.2 in detrusor overactivity in a model of obesity associated with insulin resistance in mice. PLoS One7:e48507 (2012).
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