why BSA is need ed in some resriction enzymes reaction
Dr. Duncan Clark
duncan at genesys.demon.co.uk
Sat Oct 4 03:58:12 EST 1997
In article <34351DD9.17B7 at ibm.net>, Philip Cheng <lsu0640 at ibm.net>
>Does anyone know why BSA is needed in some restriction enzymes reaction?
Most RE's have specific activities in excess of 1,000,000 u/mg therefore
your 5u represents just 5ng of protein. BSA may help decrease the amount
of non-specific absorption of the RE to plastics or just stabilizes it
through increasing the general protein concn. in the reaction.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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