- Konstantinov, Konstantin N. and Sitdikov, Ravil A. and Lopez, Gabriel P. and Atanassov, Plamen and Rubin, Robert L., Rapid detection of anti-chromatin autoantibodies in human serum using a portable electrochemical biosensor,
BIOSENSORS & BIOELECTRONICS, vol. 24 no. 7
pp. 1949--1954 [doi] .
(last updated on 2010/02/08)
The current system employed electrochemical sensor technology for the measurement of autoantibodies in human sera. Anti-chromatin antibodies are an early and sensitive indicator of systemic lupus erythematosus (SLE) and are typically quantified using enzyme-linked immunosorbent assays (ELISA). Electrochemical detection compared favorably with ELISA using a sample of 30 SLE sera (r = 0.9), and nonspecific binding by normal serum immunoglobulin was undetectable. The electrochemical sensor assay required <20 min processing time and utilized a hand-held apparatus with a disposable electrode. These results demonstrate the applicability of this technology to the rapid measurement of a clinically relevant analyte with an apparatus of potential simplicity and low cost. (C) 2008 Elsevier B.V. All rights reserved.