Determination of M. tuberculosis growth in lungs from mice immunized with liposomes by different routes. BALB/c mice were immunized by: a) a single dose or two doses of liposomes (50 μg of DNA each) by intramuscular injection at a 15-day interval, b) a single dose of liposomes (25 μg) by intranasal instillation, c) four doses of naked DNA (100 μg each) by intramuscular route at a 15-day interval or d) one dose of BCG (Moreau strain) given by subcutaneous injection of about 105 live bacteria in 100 μl saline. Thirty days after the last dose, mice were challenged intra-tracheally with M. tuberculosis H37RV and 30 days post infection we performed CFU analysis. Data represent the mean log10 CFU counts ± SD of six mice per group of one of three independent experiments. *p < 0.01 were considered significant when compared to the saline group.
Rosada et al. BMC Immunology 2008 9:38 doi:10.1186/1471-2172-9-38