Enhanced Accumulation of Long-Circulating Liposomes Modified with Nucleosome-Specific Monoclonal Antibody 2c5 in Various Tumors in Mice
T.A. ElBayoumi and V.P. Torchilin
Northeastern University, US
long-circulating liposomes, nucleosomes, monoclonal antibody
The EPR-dependent passive tumor-targeting of long-circulating liposomes was further improved for the delivery of imaging agents by post-insertion coupling to the anti-cancer monoclonal antibody 2C5 (mAb 2C5) with nuclesome-restricted activity, that can recognize the surface of various tumor but not normal cells and specifically targets pharmaceutical carriers to tumor cells in vitro and in vivo. These mAb 2C5-modified PEG-liposomes demonstrated 3-8 fold increase in the in vitro specific cell binding with various cancer cell lines of diverse origins. In addition, using 111-In-labeled liposomes, the biodistribution data along with whole–body γ-scintigraphic imaging of mice implanted with different tumors over 24 hour period, reveal the markedly faster and superior in vivo tumor accumulation of the mAb 2C5-radioliposomes than the 2C5- free radioformulations in all tested tumor models, of both murine and human origins. This formulation shows the capability of specifically delivering cargos of various imaging and contrast agents, into a diversity of tumors, hence, enhancing the specificity and the signal- to-nose ratio of the tumor imaging technique.
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Nanotech 2006 Conference Program Abstract