Submitted to: Society of Nematology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: July 9, 1999
Publication Date: N/A
Technical Abstract: Fungal natural products are being isolated that reduce root-knot nematode (Meloidogyne incognita) egg hatch and larval mobility in vitro. Amberlite XAD gel was used to extract culture broths of Fusarium equiseti and a second fungal species. Nematode-antagonistic compounds in the Fusarium extract were separated from polar constituents by partitioning between water-methanol and ethyl acetate. Fractionation of the ethyl acetate extract on a series of silica gel chromatography columns produced fractions that reduced egg hatch by 66% when tested at 50 micrograms/ml. Components of the active Fusarium fractions are being purified with reversed-phase HPLC. The XAD extract of the second fungal species yielded a methanol-soluble fraction that reduced egg hatch by 55% when tested at 250 micrograms/ml. Further bioassay-directed separation of the methanol-soluble fraction with reversed-phase column chromatography is expected to yield a more potent fraction.