Chimerism Assessment by Polymorphic Microsatellite Markers
- To evaluate and monitor engraftment after hematopoietic stem cell transplant using total cells (both peripheral blood and bone marrow) and ,sorted T- and myeloid cells (bone marrow only). Detection of microsatelllite markers that are exclusively donor in origin indicates successful engraftment. Serial values showing a decline i engraftment of donor cells may predict a graft-failure in appropriate clinical settings.
- To identify the source of successful engraftment when more than one donors are used from the hematopoietic stem cell transplant.
Initial screening of pre-transplant and donor samples involves PCR for eight microsatellite markers to identify an informative marker that can be used for subsequent analysis.
Peripheral blood samples are required from both donor and recipient.
Five to seven working days
- 10 ml peripheral blood in purple top tube (EDTA Vacutainer), sent on wet ice
- 2-5 ml of bone marrow aspirate, sent on wet ice
81265, 81267, 81268
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