peptide mapping - (Sep/11/2005 )
can any1 give the protocol for peptide mapping
You mean biopanning? Affinity-screening of phage-displayed peptide libraries?
Paper is called Felici F et al 1995 Biotech Ann Rev 1, 149-183
its actually peptide mapping of an enzyme.by the way what is biopanning?
Do you mean epitope mapping with synthetic peptides like the PEPSCAN method?
no its not epitope mapping.enzyme is used as a sample and its peptide map is generated,fragmentation of the desired protein,separation of resulting peptides and their identification by running it on a chromatography.
I know this post is about a month old, but if you are still searching.....the Salk institute has a link with a basic protocol
thanks alot Rafflez.