I collected brain tissue sections from a fixed mouse brain several weeks ago and mounted them on slides, but never used them. I was hoping I could perform a Nissl stain, but usually I only let sections like this dry out overnight (or a few hours on a slide warmer). Are these sections ok to use? Is there something I can do to rescue the issue--like hydrate the sections then let them dry again?
Details, if they matter: Fixed by PFA perfusion, post-fixed in PFA, whole brain mounted in agar and sectioned with vibratome (75u coronal) and the mounted sections were stored at RT, protected in a slide box.
Edited by Eureka0226, 11 March 2014 - 11:23 AM.