Affiliation: University of Munich
- Intranuclear changes in cancer cellsDavid M Gilbert
Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA
Genome Biol 8:312. 2007
- Organization of early and late replicating DNA in human chromosome territoriesD Zink
Institut für Anthropologie und Humangenetik, LMU Munchen, Goethestrasse 31, Munchen, D 80336, Germany
Exp Cell Res 247:176-88. 1999..These findings are compatible with the hypothesis that DNA organized into replicon clusters remains stably aggregated in non-S-phase cells...
- Transcription-dependent spatial arrangements of CFTR and adjacent genes in human cell nucleiDaniele Zink
Ludwig Maximilians University Munich, Department of Biology II, 80336 Munich, Germany
J Cell Biol 166:815-25. 2004..Together, the results show that small chromosomal subregions can display highly flexible nuclear organizations that are regulated at the level of individual genes in a transcription-dependent manner...
- Nuclear structure in cancer cellsDaniele Zink
University of Munich LMU, Department of Biology II, Goethestr 31, 80336 Munich, Germany
Nat Rev Cancer 4:677-87. 2004..Advances in understanding nuclear structure have revealed insights into the process of malignant transformation and provide a basis for the development of new diagnostic tools and therapeutics...
- The temporal program of DNA replication: new insights into old questionsDaniele Zink
Department Biologie II, Ludwig Maximilians Universitat Munchen, Biozentrum, Planegg Martinsried, Germany
Chromosoma 115:273-87. 2006..These clear predictions, and the fact that the tools are at hand now to further test them, open an avenue towards solving the long standing problem on how replication timing is determined in metazoan cells...
- Visualizing chromatin and chromosomes in living cellsDaniele Zink
Department Biologie 2, Universität München LMU, Goethestrasse 31, 80336, Munich, Germany
Methods 29:42-50. 2003..Appropriate labeling procedures as well as cell chamber systems and important controls for live cell microscopy are described...
- Live-cell microscopy of single nuclear chromosomes and genome compartments: evaluation of labeling procedure and imaging conditionsC Fauth
LMU Munchen, Institut für Anthropologie und Humangenetik, Munchen, Germany
Cytometry 45:214-24. 2001..J Cell Biol 146:1211-1226, 1999). We characterized the potential artificial impact of this approach on chromosome structure and dynamics. We also evaluated potential sources of artifacts in corresponding live-cell imaging...
- Large-scale chromatin fibers of living cells display a discontinuous functional organizationN Sadoni
, , Munich, Germany
Chromosoma 110:39-51. 2001..In particular, Cy3-labeled foci appeared as discontinuous regions of large-scale fibers. These results demonstrate that large-scale chromatin fibers have discontinuous functional characteristics...
- Targeted deficiency of the transcriptional activator Hnf1alpha alters subnuclear positioning of its genomic targetsReini F Luco
Genomic Programming of Beta Cells Laboratory, Institut d Investigacions August Pi i Sunyer, Barcelona, Spain
PLoS Genet 4:e1000079. 2008..We have also revealed a defect in subnuclear gene positioning in a model of a human transcription factor disease...
- Conserved patterns of nuclear compartmentalization are not observed in the chordate OikopleuraFabio Spada
Sars Centre for Marine Molecular Biology, Bergen High Technology Centre, University of Bergen, Thormøhlensgt 55, Bergen, Norway
Biol Cell 99:273-87. 2007..Recent results from a limited number of eukaryotic model organisms suggest that major principles governing spatial organization of the genome in functionally distinct nuclear compartments are conserved through evolution...
- Architectural reorganization of the nuclei upon transfer into oocytes accompanies genome reprogrammingCatherine Martin
UMR 13 1198 Biologie du Développement, Institut National de Recherche Agronomique, Domaine de Vilvert, Jouy en Josas, France
Mol Reprod Dev 73:1102-11. 2006..We could also establish that the adequate distribution of chromosomal territories at the pronuclear stage seems important for the development until blastocyst...
- Transcription-dependent spatial arrangements of CFTR and conserved adjacent loci are not conserved in human and murine nucleiNicolas Sadoni
Department of Biology II, Ludwig Maximilians University Munich, Biocenter, Planegg Martinsried, Germany
Chromosoma 117:381-97. 2008..Furthermore, the results suggest that the way how nuclear positioning contributes to the regulation of conserved loci can be different in different vertebrate species...
- Genome restructuring in mouse embryos during reprogramming and early developmentCatherine Martin
UMR 13 1198 Biologie du Développment et Reproduction, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
Dev Biol 292:317-32. 2006....
- The replication timing of CFTR and adjacent genesAndreas Englmann
Ludwig Maximilians University Munich, Department of Biology II, Grosshaderner Str 2, 82152, Planegg Martinsried, Germany
Chromosome Res 13:183-94. 2005..Furthermore, the results imply a specific functional order of nuclear changes related to switches in replication timing...
- Stable chromosomal units determine the spatial and temporal organization of DNA replicationNicolas Sadoni
University of Munich LMU, Department Biology II, Grosshaderner Str 2, 82152 Planegg Martinsried, Germany
J Cell Sci 117:5353-65. 2004..These findings imply that the specific nuclear substructure of chromosomes and the order of their stable subunits determine the spatiotemporal organization of DNA replication...
- Nascent RNA synthesis in the context of chromatin architectureNicolas Sadoni
Universität München LMU, Department Biologie II, Goethestr 31, 80336 Munchen, Germany
Chromosome Res 12:439-51. 2004..However, the results are in agreement with the emerging view that even condensed chromatin domains display a highly dynamic organization and are not a physical barrier for transcription factors...
- Comparative analysis of the functional genome architecture of animal and plant cell nucleiChristoph Mayr
University of Munich LMU, Department Biology II, Goethestr 31, 80336 Munich, Germany
Chromosome Res 11:471-84. 2003..There are, however, differences between animals and plants with regard to histone acetylation patterns and the nuclear distribution of late-replicating chromatin...
- The nuclear organization of Polycomb/Trithorax group response elements in larval tissues of Drosophila melanogasterElena Fedorova
Department Biologie II, Ludwig Maximilians Universitat Munchen, Grosshaderner Str 2, 82152, Planegg Martinsried, Germany
Chromosome Res 16:649-73. 2008....
- Association of p53 and MSH2 with recombinative repair complexes during S phaseDaniele Zink
Institut für Anthropologie und Humangenetik LMU München, Goethestr 31, 80336 Munchen, Germany
Oncogene 21:4788-800. 2002..Our data suggest that p53 is linked similarly to homologous recombination as MSH2 and provide further evidence for the new concept of a dual role of p53 in the regulation of growth and repair...