1R) is a eukaryotic membrane protein that functions as an inter-organelle signaling modulator and chaperone. Here we report an improved expression of S1R in Escherichia coli as a fusion to maltose binding protein (MBP) and a high-yield purification. Variants with linking amino acid sequences consisting of 0-5 alanine residues between MBP… (More)
- Katarzyna A. Gromek, Fabian P. Suchy, +7 authors Brian G. Fox
- The Journal of biological chemistry
- 2014
Sigma-1 receptor (S1R) is a mammalian member of the ERG2 and sigma-1 receptor-like protein family (pfam04622). It has been implicated in drug addiction and many human neurological disorders, including Alzheimer and Parkinson diseases and amyotrophic lateral sclerosis. A broad range of synthetic small molecules, including cocaine, (+)-pentazocine,… (More)
- Emily T. Beebe, Shin-ichi Makino, +5 authors Brian G. Fox
- New biotechnology
- 2011
The use of the Protemist XE, an automated discontinuous-batch protein synthesis robot, in cell-free translation is reported. The soluble Galdieria sulphuraria protein DCN1 was obtained in greater than 2mg total synthesis yield per mL of reaction mixture from the Protemist XE, and the structure was subsequently solved by X-ray crystallography using material… (More)
- Ronnie O. Frederick, Lai F. Bergeman, +17 authors Brian G. Fox
- Journal of Structural and Functional Genomics
- 2007
A simple approach that allows cost-effective automated purification of recombinant proteins in levels sufficient for functional characterization or structural studies is described. Studies with four human stem cell proteins, an engineered version of green fluorescent protein, and other proteins are included. The method combines an expression vector (pVP62K)… (More)
- Brian G. Fox, Thomas E. Malone, +44 authors Zolt Zolnai
- Proteins
- 2005
- John L. Markley, David J. Aceti, +11 authors Zsolt Zolnai
- Journal of Structural and Functional Genomics
- 2008
The Center for Eukaryotic Structural Genomics (CESG) is a “specialized” or “technology development” center supported by the Protein Structure Initiative (PSI). CESG’s mission is to develop improved methods for the high-throughput solution of structures from eukaryotic proteins, with a very strong weighting toward human proteins of biomedical relevance.… (More)
- David J. Aceti, Craig A. Bingman, +18 authors Brian G. Fox
- Journal of Structural and Functional Genomics
- 2015… (More)
- Eduard Bitto, Craig A. Bingman, +13 authors George N. Phillips
- Acta crystallographica. Section F, Structural…
- 2005
The crystal structure of the gene product of At3g21360 from Arabidopsis thaliana was determined by the single-wavelength anomalous dispersion method and refined to an R factor of 19.3% (Rfree = 24.1%) at 2.4 A resolution. The crystal structure includes two monomers in the asymmetric unit that differ in the conformation of a flexible domain that spans… (More)
- Brian G. Fox, Arnold E. Ruoho, +7 authors John G. Primm
- 2014"The gubmit" knows where you live. The gubmit doesn't know where you are exactly at every given time. And I know you will now proceed to smugly say "uhhh do you guys have phones" - yes, but you can just leave yours at home or not own one at all. Unless you live in Australia where they made it illegal because of corona scare. Literally.

&gt;You're aware they already do that, especially if you live in America?

No, private businesses couldn't get your info without breaking the law before.

I didn't explain myself properly. Mgtow, incel and PUA/theredpill are part of the manosphere. The manosphere is'redpilled' in that they claim to see reality how it truly is.

The concept of AI partners (also sex bots) is not necessarily a redpilled concept but it's popular among the manosphere (which yes, claims to be redpilled). It's popular among MGTOW and incel but not among PUA, for instance.

I'm not trying to shame incel or MGTOW here, just saying it's popular among those and not necessarily redpilled. Personally, I think AI or sex bots would be a massive cope and clear reality avoidance. If they could chose, despite being redpilled, I think they'd chose a decent girlfriend over an AI/bot. A perfect bot could be pleasant and work, if it's really perfect they'd probably not feel alone, but they'd always know that's not real and it's programmed so they could not get validation from it. Like it or not, validation from the sex you are attracted to, matters a lot.
That said, a very redpilled guy could consider that his values don't align with women's values and therefore not need validation from a real woman (though I'm not sure that's possible since we are wired to need it)We are currently debating Gay Marriage, LGBT rights, and DOMA in our largest court. The most prevalent argument against Gay Marriage and in support of DOMA is that marriage is something ordained by God. First of all I would ask, which god, whose god?
But beyond that, marriage is a contract between the state and the individuals being married. In every state in this union a couple can be married by a Justice of the Peace or even a Notary Public with no mention of a god at all. Moreover, those same couples, were they to enter a church, would have their marriages recognized as binding. In short, the church and those who hold gods so dear recognize civil marriages that are devoid of a god.
What are they recognizing and honoring? They are giving unto Caesar that which is Caesar’s. They are recognizing the state’s ability to declare a marriage. As such, it becomes clear that marriage is not the sole resource for procreation and it becomes clear that marriage is not the sole heir of religion. Marriage is an arrangement, for legal purposes, between two people and the state.
For those who fear the slippery slope, I again point out that we can orchestrate the contract in any way we choose. We can say two, and only two people, and only people. All other way out concerns are hence answered.
It is time to realize that all protestations about god and marriage or procreation and marriage have nothing to do with a contract between the state and two loving people.<commit_before>/*
 * LDAP Chai API
 * Copyright (c) 2006-2010 Novell, Inc.
 *
 * This library is free software; you can redistribute it and/or
 * modify it under the terms of the GNU Lesser General Public
 * License as published by the Free Software Foundation; either
 * version 2.1 of the License, or (at your option) any later version.
 *
 * This library is distributed in the hope that it will be useful,
 * but WITHOUT ANY WARRANTY; without even the implied warranty of
 * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the GNU
 * Lesser General Public License for more details.
 *
 * You should have received a copy of the GNU Lesser General Public
 * License along with this library; if not, write to the Free Software
 * Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA  02111-1307  USA
 */

package com.novell.ldapchai.impl.ad.entry;

import com.novell.ldapchai.*;
import com.novell.ldapchai.exception.*;
import com.novell.ldapchai.impl.AbstractChaiUser;
import com.novell.ldapchai.provider.ChaiProvider;
import com.novell.ldapchai.provider.ChaiSetting;
import com.novell.ldapchai.util.DefaultChaiPasswordPolicy;
import com.novell.ldapchai.util.SearchHelper;
import com.novell.ldapchai.util.StringHelper;

import java.io.UnsupportedEncodingException;
import java.util.*;
import java.util.regex.Matcher;
import java.util.regex.Pattern;

class UserImpl extends AbstractChaiUser implements User, Top, ChaiUser {

    // @todo: replace with @UserAccountControl
    private static final int COMPUTED_ACCOUNT_CONTROL_ACCOUNT_ACTIVE = 0x0002;
    private static final int COMPUTED_ACCOUNT_CONTROL_UC_LOCKOUT = 0x0010;
    private static final int COMPUTED_ACCOUNT_CONTROL_UC_PASSWORD_EXPIRED = 0x800000;
    private static final int ADS_UF_DONT_EXPIRE_PASSWD = <PASSWORD>;
    private static final String LDAP_SERVER_POLICY_HINTS_OID = "1.2.840.113556.1.4.2066";

    UserImpl(final String userDN, final ChaiProvider chaiProvider)
    {
        super(userDN, chaiProvider);
    }

    