,Unnamed: 0,Method,Vial 1,Vial 2,Ty21a,Ty2,E.coli
0,Mircobiological,API 20 E,S. Typhi,S. Typhi,S. Typhi,S. Typhi,E. coli
1,,Colony appearance on Bromothymol blue agar,Blue,Blue,Blue,Yellow,Blue
2,,(+ 1% galactose),,,,,
3,Biochemical,Minimal media + cysteine + thiamine +,No growth,No growth,No growth,Growth,No growth
4,,tryptophan,,,,,
5,,Minimal media + cysteine + thiamine +,Growth,Growth,Growth,Growth,Growth
6,,tryptophan + valine + isolleucine,,,,,
7,,Heat stress at 55C for 20 min,Sensitive,Sensitive,Sensitive,Resistant,Sensitive
8,,Oxidative stress in 0.3% H2O2 for 20 min,Sensitive,Sensitive,Sensitive,Resistant,Sensitive
9,,Gllactose (1%) induced bacteriolysis,Sensitive,Sensitive,Sensitive,Resistant,ND
10,Immunological,"9,12 O-antigen agglutination",+,+,+,+,ND
11,,Vi antigen agglutination,-,-,-,+,ND
12,,PA Expression (colony and western blot),+,+,-,ND,ND
13,Genetic,16S rDNA sequence,Identical to,Identical to,Ty21a,Ty21a,ND
14,,,Ty21a,Ty21a,,,
15,,gaNE DNA sequence,Identical to,Identical to,Ty21a (T367C;,Ty21a (wild,ND
16,,,Ty21a,Ty21a,C442),type),
17,,Chromasomally intergrated PA expression-,+,+,-,-,ND
18,,secretion cassette (PCR),,,,,
