<s>
Random	B-Application
coil	I-Application
index	I-Application
(	O
RCI	O
)	O
predicts	O
protein	O
flexibility	O
by	O
calculating	O
an	O
inverse	O
weighted	O
average	O
of	O
backbone	O
secondary	O
chemical	O
shifts	O
and	O
predicting	O
values	O
of	O
model-free	O
order	O
parameters	O
as	O
well	O
as	O
per-residue	O
RMSD	O
of	O
NMR	O
and	O
molecular	O
dynamics	O
ensembles	O
from	O
this	O
parameter	O
.	O
</s>
<s>
The	O
actual	O
calculation	O
of	O
the	O
RCI	O
involves	O
several	O
additional	O
steps	O
including	O
the	O
smoothing	O
of	O
secondary	O
shifts	O
over	O
several	O
adjacent	O
residues	O
,	O
the	O
use	O
of	O
neighboring	O
residue	O
corrections	O
,	O
chemical	B-Application
shift	I-Application
re-referencing	I-Application
,	O
gap	O
filling	O
,	O
chemical	O
shift	O
scaling	O
and	O
numeric	O
adjustments	O
to	O
prevent	O
divide-by-zero	B-Algorithm
problems	O
.	O
</s>
