<s>
OLIGO	B-Application
Primer	I-Application
Analysis	I-Application
Software	I-Application
was	O
the	O
first	O
publicly	O
available	O
software	O
for	O
DNA	O
primer	O
design	O
.	O
</s>
<s>
The	O
first	O
papers	O
describing	O
this	O
software	O
were	O
published	O
in	O
1989	O
and	O
1990	O
,	O
and	O
consecutive	O
upgrades	O
in	O
the	O
1990s	O
and	O
2000s	O
,	O
all	O
have	O
been	O
cited	O
together	O
over	O
600	O
times	O
in	O
scientific	O
journals	O
and	O
over	O
500	O
times	O
in	O
patents	O
(	O
according	O
to	O
Scopus	B-Library
)	O
.	O
</s>
<s>
Oligo	B-Application
Primer	I-Application
Analysis	I-Application
Software	I-Application
has	O
been	O
used	O
in	O
various	O
scientific	O
studies	O
(	O
as	O
cited	O
by	O
examples	O
of	O
recent	O
publications	O
)	O
,	O
among	O
them	O
for	O
:	O
real	O
time	O
PCR	O
,	O
apoptosis	O
studies	O
,	O
antigen	O
typing	O
,	O
species	O
identification	O
,	O
studies	O
on	O
species	O
evolution	O
,	O
measuring	O
mRNA	O
expression	O
levels	O
,	O
oligonucleotide-based	O
array	O
hybridization	O
studies	O
,	O
degenerate	O
primer	O
studies	O
,	O
microsatellite	O
analysis	O
,	O
DNA	O
microarray	O
detection	O
,	O
inverse	O
PCR	O
,	O
genome	O
walking	O
,	O
nucleotide	O
polymorphisms	O
studies	O
,	O
detection	O
of	O
microorganisms	O
or	O
viruses	O
,	O
genotyping	O
,	O
cloning	O
,	O
vector	O
(	O
gene	O
)	O
construction	O
,	O
genome	O
sequencing	O
,	O
detection	O
of	O
mutants	O
or	O
intraspecific	O
variability	O
,	O
genetic	O
disease	O
studies	O
,	O
siRNA	O
and	O
gene	O
silencing	O
,	O
FISH	O
analysis	O
(	O
single	O
cell	O
expression	O
study	O
)	O
,	O
scorpion	O
probes	O
,	O
and	O
development	O
of	O
new	O
DNA	O
amplification	O
methods	O
.	O
</s>
