<s>
In	O
biochemistry	O
,	O
the	O
Lineweaver	B-Application
–	I-Application
Burk	I-Application
plot	I-Application
(	O
or	O
double	B-Application
reciprocal	I-Application
plot	I-Application
)	O
is	O
a	O
graphical	O
representation	O
of	O
the	O
Michaelis	O
–	O
Menten	O
equation	O
of	O
enzyme	O
kinetics	O
,	O
described	O
by	O
Hans	O
Lineweaver	O
and	O
Dean	O
Burk	O
in	O
1934	O
.	O
</s>
<s>
The	O
double	B-Application
reciprocal	I-Application
plot	I-Application
distorts	O
the	O
error	O
structure	O
of	O
the	O
data	O
,	O
and	O
is	O
therefore	O
not	O
the	O
most	O
accurate	O
tool	O
for	O
the	O
determination	O
of	O
enzyme	O
kinetic	O
parameters	O
.	O
</s>
<s>
While	O
the	O
Lineweaver	B-Application
–	I-Application
Burk	I-Application
plot	I-Application
has	O
historically	O
been	O
used	O
for	O
evaluation	O
of	O
the	O
parameters	O
,	O
together	O
with	O
the	O
alternative	O
linear	O
forms	O
of	O
the	O
Michaelis	O
–	O
Menten	O
equation	O
such	O
as	O
the	O
Hanes-Woolf	B-Application
plot	I-Application
or	O
Eadie	O
–	O
Hofstee	O
plot	O
,	O
all	O
linearized	O
forms	O
of	O
the	O
Michaelis	O
–	O
Menten	O
equation	O
should	O
be	O
avoided	O
to	O
calculate	O
the	O
kinetic	O
parameters	O
.	O
</s>
<s>
The	O
Lineweaver-Burk	B-Application
plot	I-Application
derives	O
from	O
a	O
transformation	O
of	O
the	O
Michaelis-Menten	O
equation	O
,	O
</s>
<s>
When	O
used	O
for	O
determining	O
the	O
type	O
of	O
enzyme	O
inhibition	O
,	O
the	O
Lineweaver	B-Application
–	I-Application
Burk	I-Application
plot	I-Application
can	O
distinguish	O
between	O
competitive	O
,	O
pure	O
non-competitive	O
and	O
uncompetitive	O
inhibitors	O
.	O
</s>
<s>
This	O
can	O
be	O
seen	O
on	O
the	O
Lineweaver	B-Application
–	I-Application
Burk	I-Application
plot	I-Application
as	O
an	O
increased	O
ordinate	O
intercept	O
with	O
no	O
effect	O
on	O
the	O
abscissa	O
intercept	O
,	O
as	O
pure	O
noncompetitive	O
inhibition	O
does	O
not	O
effect	O
substrate	O
affinity	O
.	O
</s>
<s>
This	O
can	O
be	O
seen	O
on	O
the	O
Lineweaver	B-Application
–	I-Application
Burk	I-Application
plot	I-Application
as	O
an	O
increased	O
intercept	O
on	O
the	O
ordinate	O
with	O
no	O
change	O
in	O
slope	O
.	O
</s>
<s>
Although	O
the	O
Lineweaver-Burk	B-Application
plot	I-Application
is	O
useful	O
,	O
it	O
shows	O
the	O
experimental	O
error	O
very	O
badly	O
.	O
</s>
