<s>
In	O
biochemistry	O
,	O
a	O
Hanes	B-Application
–	I-Application
Woolf	I-Application
plot	I-Application
,	O
Hanes	O
plot	O
,	O
or	O
plot	O
of	O
against	O
,	O
is	O
a	O
graphical	O
representation	O
of	O
enzyme	O
kinetics	O
in	O
which	O
the	O
ratio	O
of	O
the	O
initial	O
substrate	O
concentration	O
to	O
the	O
reaction	O
velocity	O
is	O
plotted	O
against	O
.	O
</s>
<s>
we	O
can	O
take	O
reciprocals	O
of	O
both	O
sides	O
of	O
the	O
equation	O
to	O
obtain	O
the	O
equation	O
underlying	O
the	O
Lineweaver	B-Application
–	I-Application
Burk	I-Application
plot	I-Application
:	O
</s>
<s>
Like	O
other	O
techniques	O
that	O
linearize	O
the	O
Michaelis	O
–	O
Menten	O
equation	O
,	O
the	O
Hanes	B-Application
–	I-Application
Woolf	I-Application
plot	I-Application
was	O
used	O
historically	O
for	O
rapid	O
determination	O
of	O
the	O
kinetic	O
parameters	O
,	O
and	O
'	O
,	O
but	O
it	O
has	O
been	O
largely	O
superseded	O
by	O
nonlinear	O
regression	O
methods	O
that	O
are	O
significantly	O
more	O
accurate	O
and	O
no	O
longer	O
computationally	O
inaccessible	O
.	O
</s>
