<s>
CLARITY	B-Algorithm
is	O
a	O
method	O
of	O
making	O
tissue	O
transparent	O
using	O
acrylamide-based	O
hydrogels	O
built	O
from	O
within	O
,	O
and	O
linked	O
to	O
,	O
the	O
tissue	O
,	O
and	O
as	O
defined	O
in	O
the	O
initial	O
paper	O
,	O
represents	O
"	O
transformation	O
of	O
intact	O
biological	O
tissue	O
into	O
a	O
hybrid	O
form	O
in	O
which	O
specific	O
components	O
are	O
replaced	O
with	O
exogenous	O
elements	O
that	O
provide	O
new	O
accessibility	O
or	O
functionality	O
"	O
.	O
</s>
<s>
When	O
accompanied	O
with	O
antibody	O
or	O
gene-based	O
labeling	O
,	O
CLARITY	B-Algorithm
enables	O
highly	O
detailed	O
pictures	O
of	O
the	O
protein	O
and	O
nucleic	O
acid	O
structure	O
of	O
organs	O
,	O
especially	O
the	O
brain	O
.	O
</s>
<s>
Several	O
published	O
papers	O
have	O
applied	O
the	O
CLARITY	B-Algorithm
method	O
to	O
a	O
wide	O
range	O
of	O
tissues	O
and	O
disease	O
states	O
such	O
as	O
immuno-oncology	O
for	O
human	O
breast	O
cancer	O
,	O
Alzheimer	O
's	O
disease	O
human	O
brains	O
,	O
mouse	O
spinal	O
cords	O
,	O
multiple	O
sclerosis	O
animal	O
models	O
,	O
and	O
plants	O
.	O
</s>
<s>
CLARITY	B-Algorithm
has	O
also	O
been	O
combined	O
with	O
other	O
technologies	O
to	O
develop	O
new	O
microscopy	O
methods	O
including	O
confocal	O
expansion	O
microscopy	O
,	O
SPIM	O
light	O
sheet	O
microscopy	O
,	O
and	O
CLARITY-optimized	O
light	O
sheet	O
microscopy	O
(	O
COLM	O
)	O
.	O
</s>
<s>
The	O
process	O
of	O
applying	O
CLARITY	B-Algorithm
imaging	O
begins	O
with	O
a	O
postmortem	O
tissue	O
sample	O
.	O
</s>
<s>
Lipophilic	O
dyes	O
as	O
DiI	O
are	O
removed	O
,	O
however	O
there	O
are	O
CLARITY-compatible	O
lipophilic	O
dyes	O
that	O
can	O
be	O
fixated	O
to	O
neighbouring	O
proteins	O
.	O
</s>
<s>
Standard	O
confocal	O
,	O
two-photon	O
,	O
or	O
light-sheet	O
imaging	O
methods	O
are	O
all	O
suitable	O
to	O
then	O
detect	O
the	O
fluorescence	O
emitted	O
down	O
to	O
the	O
scale	O
of	O
protein	O
localization	O
,	O
thus	O
resulting	O
in	O
the	O
final	O
highly	O
detailed	O
and	O
three-dimensional	O
images	O
that	O
CLARITY	B-Algorithm
produces	O
.	O
</s>
<s>
In	O
terms	O
of	O
brain	O
imaging	O
,	O
the	O
ability	O
for	O
CLARITY	B-Algorithm
imaging	O
to	O
reveal	O
specific	O
structures	O
in	O
such	O
unobstructed	O
detail	O
has	O
led	O
to	O
promising	O
avenues	O
of	O
future	O
applications	O
including	O
local	O
circuit	B-General_Concept
wiring	O
(	O
especially	O
as	O
it	O
relates	O
to	O
the	O
Connectome	O
Project	O
)	O
,	O
relationships	O
between	O
neural	O
cells	O
,	O
roles	O
of	O
subcellular	O
structures	O
,	O
better	O
understanding	O
of	O
protein	O
complexes	O
,	O
and	O
imaging	O
of	O
nucleic	O
acids	O
and	O
neurotransmitters	O
.	O
</s>
<s>
An	O
example	O
of	O
a	O
discovery	O
made	O
through	O
CLARITY	B-Algorithm
imaging	O
is	O
a	O
peculiar	O
'	O
ladder	O
 '	O
pattern	O
where	O
neurons	O
connected	O
back	O
to	O
themselves	O
and	O
their	O
neighbors	O
,	O
which	O
has	O
been	O
observed	O
in	O
animals	O
to	O
be	O
connected	O
to	O
autism-like	O
behaviors	O
.	O
</s>
<s>
The	O
CLARITY	B-Algorithm
technique	O
has	O
expanded	O
to	O
several	O
applications	O
beyond	O
the	O
brain	O
.	O
</s>
<s>
Numerous	O
modifications	O
have	O
been	O
published	O
to	O
build	O
upon	O
the	O
initial	O
publications	O
and	O
efforts	O
have	O
been	O
made	O
both	O
academically	O
and	O
within	O
the	O
biotech	O
industry	O
to	O
apply	O
broad-scope	O
applications	O
to	O
CLARITY	B-Algorithm
.	O
</s>
<s>
CLARITY	B-Algorithm
continues	O
to	O
gain	O
traction	O
as	O
an	O
emergent	O
technology	O
that	O
can	O
provide	O
powerful	O
insights	O
into	O
clinical	O
diagnostics	O
in	O
the	O
future	O
.	O
</s>
<s>
CLARITY	B-Algorithm
can	O
be	O
used	O
with	O
little	O
or	O
no	O
modifications	O
to	O
clear	O
most	O
other	O
organs	O
such	O
as	O
liver	O
,	O
pancreas	O
,	O
spleen	O
,	O
testis	O
,	O
and	O
ovaries	O
and	O
other	O
species	O
such	O
as	O
zebrafish	O
.	O
</s>
<s>
Although	O
the	O
CLARITY	B-Algorithm
procedure	O
has	O
attained	O
unprecedented	O
levels	O
of	O
protein	O
retention	O
after	O
lipid	O
extraction	O
,	O
the	O
technique	O
still	O
loses	O
an	O
estimated	O
8%	O
of	O
proteins	O
per	O
instance	O
of	O
detergent	O
electrophoresis	O
.	O
</s>
<s>
Time	O
has	O
often	O
been	O
cited	O
as	O
a	O
limiting	O
factor	O
and	O
disadvantage	O
to	O
using	O
the	O
CLARITY	B-Algorithm
technique	O
;	O
however	O
,	O
several	O
academic	O
and	O
biotech	O
companies	O
(	O
Logos	O
Bioscience	O
,	O
ClearLight	O
Biotechnologies	O
)	O
have	O
continued	O
to	O
develop	O
and	O
optimize	O
CLARITY	B-Algorithm
reagents	O
that	O
significantly	O
truncated	O
the	O
immunostaining	O
time	O
frame	O
reducing	O
the	O
process	O
from	O
six	O
weeks	O
to	O
as	O
quickly	O
as	O
a	O
week	O
depending	O
on	O
sample	O
type	O
.	O
</s>
