<s>
Beacon	B-Application
Designer	I-Application
designs	O
highly	O
specific	O
and	O
efficient	O
primers	O
and	O
probes	O
for	O
real-time	O
PCR	O
(	O
polymerase	O
chain	O
reaction	O
)	O
assays	O
.	O
</s>
<s>
Beacon	B-Application
Designer	I-Application
program	O
has	O
built-in	O
support	O
for	O
homology	O
avoidance	O
using	O
the	O
standard	O
web	O
BLAST	O
service	O
or	O
the	O
set-up	O
of	O
a	O
desktop	O
BLAST	O
feature	O
for	O
the	O
identification	O
of	O
regions	O
that	O
are	O
significantly	O
cross-homologous	O
with	O
the	O
target	O
gene	O
sequence	O
.	O
</s>
<s>
Beacon	B-Application
Designer	I-Application
has	O
been	O
used	O
in	O
workshops	O
and	O
conferences	O
related	O
to	O
real-time	O
PCR	O
study	O
as	O
a	O
reference	O
software	O
.	O
</s>
<s>
The	O
author	O
's	O
company	O
,	O
Premier	O
Biosoft	O
has	O
also	O
launched	O
a	O
simple	O
version	O
for	O
evaluation	O
of	O
oligos	O
for	O
real	O
time	O
PCR	O
primers	O
and	O
probes	O
(	O
support	O
limited	O
to	O
TaqMan	O
and	O
SYBR	O
Green	O
primers	O
)	O
by	O
the	O
name	O
Beacon	B-Application
designer	I-Application
free	O
version	O
.	O
</s>
