Predicted vs actual MW
pxpst2+ at pitt.edu
Sat Nov 6 09:43:13 EST 1999
In article <3821AEEE.9EFC3D58 at sheffield.ac.uk>, Lee Hunt
<L.hunt at sheffield.ac.uk> wrote:
> Does anybody have explanations as to why a protein on a Western can
> differ widely from its predicted size. Other than proteolysis or removal
> of targetting regions what else could cause this?
Always remember that PAGE does not seperate purely by mass/size.
Seperation is by the charge to mass (m/z). Alter this and and you will
have abherent migration.
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