Weird Northern again!!!
cxwang at wam.umd.edu
Sun Jul 23 22:24:51 EST 2000
I used the standard hybridization condition according to the< molecular
cloning> at 65C and washed at a high stringent condition.
On Sun, 23 Jul 2000, news.mad.ttd.net wrote:
> Chunxin Wang <cxwang at wam.umd.edu> escribi=F3 en el mensaje de noticias
> Pine.GSO.4.21.0007231634430.27417-100000 at rac1.wam.umd.edu...
> > This time two new probes PCR-amplified from arabidopsis PR-1 and VSP1
> > genes, repectively, were used and the membrane was newly transferred. I
> > did the hybridization at the same time in different bags. However, the
> > result was the same: all signals came from the rRNAs. The hybridization
> > pattern was exactly the same as the EB-stained gel pattern.
> > why it happened to me so often in so short time?I was so desperate now.
> > Black
> I don=B4t know the previous messages but, which hybridization conditions =
> you using?.
> Jose Luis
> Jose Luis Martinez Ph. D.
> Departamento de Biologia Celular y Desarrollo
> Centro de Investigaciones Biologicas
> C/ Velazquez 144
> Madrid 28006
More information about the Methods