Project: Identification of cytosolic signalling proteins involved in TNF-induced mitochondrial dysfunction.
- project duration
- 01-OCT-96 – 30-SEP-00
- This project aims to identify signalling proteins involved in TNF-induced mitochondrial dysfunction in transformed cells (induction of reactive oxygen species in L929 cells or decrease in mitochondrial transmembrane potential in PC-60 cells). This will be achieved by reconstructing the signal towards the mitochondria through addition of (fractionated cytosol of TNF-treated cells to free mitochondria, followed by confocal-laser-scanning-microscopy analysis.