MJ Research 100 microliter rxn?
mikef at mjr.com
Sun Sep 24 18:25:08 EST 1995
In article <DFB3I0.5su at tzv.fal.de>, carnwath at tzv.fal.de (J.Carnwath) wrote:
>We want to use 100 microliter PCR reaction volume for our RT-PCR protocol
>and are about to purchase an MJ Cycler PTC 200. The problem is deciding
>which block configuration to purchase. Has anyone successfully used 0.2
>ml tubes with 100 microliters in the DNA Engine?
Yes, 100 microliter volumes work well in the 0.2-ml tubes. The
"calculated sample temperature" algorithm of the PTC-200 has been
validated up to 100 mircroliters with these tubes, so you can use that
Michael Finney, PhD | /\/\\_\ /\_\ | 149 Grove Street
MJ Research, Inc. | /\_\_\\ \\_\ | Watertown MA 02172
mikef at mjr.com | /_/ | 617 923 8001 x112
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