Random priming labeling Need protocol
sb at mole.bio.cam.ac.uk
Wed Jun 11 09:29:22 EST 1997
PLEASE CAN SOMEONE GIVE ME A PROTOCOL TO PREPARE THE BUFFER USED FOR RANDOM
PRIMING LABELING OF DNA.
I am interested in a protocoll that give the highest 32P incorporation?
I have made some according to the Promega manual, but I can get only 20% of
incorporation (while I can get 70% with a commercial kit).
I am interested also to know what is the reason for using Hepes and Tris
Thank you very much
University of Cambridge, Department of Genetics
Downing Street, Cambridge, CB2 3EH, England (UK)
tel +44-1223-333970, fax +44-1223-333992
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