Protein silver staining.
xianhui at cuhk.edu.hk
Wed Mar 6 08:27:16 EST 1996
I have use this protocol to stain SDS-gel. "But why do you use so high
concentration of glutaraldehyde(10%)", my colleage in once asked me. So I
reduce the concentration to 1% or even 0.5% of glutaraldehyde and get the
same result. By using a shaker and raising temperature, the time for each
step may be reduced to half of the original and the stain may be finished
in 2 hour.
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