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Differentiation Media 2X methylcellulose FCS (preselected) Ascorbic acid (5 mgml) L-Glutamine (200 mM) Liquid 15 50 gml 2mM Methylcellulose 1 15 50 gml 2 mM Page 245 192 DIFFERENTIATION OF MOUSE ES CELLS 13 MTG IMDM Reagents and Materials Liquid 4.
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