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Molecular genetics is the field of biology which studies the structure and function of genes at a molecular level. Molecular genetics employs the methods of genetics and molecular biology. It is so-called to differentiate it from other sub fields of genetics such as ecological genetics and population genetics. An important area within molecular genetics is the use of molecular information to determine the patterns of descent, and therefore the correct scientific classification of organisms: this is called molecular systematics.
Forward genetics Edit
One of the first tools available to molecular geneticists is the forward genetic screen. The aim of this technique is to identify mutations that produce a certain phenotype. A mutagen is very often used to accelerate this process. Once mutants have been isolated, the mutated gene can be molecularly identified.
Reverse genetics Edit
Main article: Reverse genetics
While forward genetic screens are productive, a more straightforward approach would be to determine the phenotype that results from mutating a given gene. This is called reverse genetics. In some organisms, such as yeast and mice, it is possible to induce the deletion of a particular gene, creating a gene knockout. Alternatives include the random induction of DNA deletions and subsequent selection for deletions in a gene of interest, the application of RNA interference and the creation of transgenic organisms that do not express a gene of interest.
- The study of macromolecules important in biological inheritance
- Forward and reverse genetics screens
- Transgenics and overexpression
- Mapping, cloning, and sequencing
- Gene expression
- Spatial and temporal regulation
- Bacterial and phage molecular genetics
- Eukaryotic molecular genetics
|* Evolutionary psychology|
|Fields of genetics|
|Genetics in psychology|
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