RE [Histonet] A question...(about IHC buffer)
The primary purpose of placing slides in buffer (for a short time) prior to
IHC staining is to equilibrate the specimen -- that is, create a suitable
environment for the immunologic reagents to interact as they would in nature.
Although the first reagent that is applied may work just fine without the pre-soak
step, its considered 'good science' to equilibrate beforehand...
Joe Myers, M.S., CT(ASCP)
[mailto:email@example.com]On Behalf Of Rosa
Sent: Friday, February 02, 2007 10:34 AM
Subject: [Histonet] A question..
What is the science behind soaking the slides in buffer before IHC
procedures? I can't find much of a reference about this, what are the advantages to
this step instead of putting the slides back into water? Thanks Rosa Fields
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