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|Document Type: ||Article|
|Title: ||Labeless Immunosensor Assay for Fluoroquinolone Antibiotics Based Upon an AC
|Authors: ||Garifallou, Goulielmos-Zois|
Higson, Seamus P. J.
Millner, Paul A.
Pinacho, Daniel G.
Gibson, Timothy D.
|Issue Date: ||2007|
|Citation: ||G.-Z. Garifallou; G. Tsekenis; F. Davis; S. P. J. Higson; P. A. Millner; D. G. Pinacho; F. Sanchez-Baeza; M.-P. Marco; T. D. Gibson. Labeless Immunosensor Assay for Fluoroquinolone Antibiotics Based Upon an AC Impedance Protocol, Analytical Letters, Volume 40, Issue 7, January 2007, pages 1412 - 1422|
|Abstract: ||This paper describes the construction of a labeless immunosensor for the
antibiotic ciprofloxacin and its interrogation using an AC impedance protocol.
Commercial screen-printed carbon electrodes were used as the basis for the
sensor. Polyaniline was electrodeposited onto the sensors and then utilized to
immobilize a biotinylated antibody for ciprofloxacin using classical avidin-
biotin interactions. Electrodes containing the antibodies were exposed to
solutions of antigen and interrogated using an AC impedance protocol. The
faradaic component of the impedance of the electrodes was found to increase with
increasing concentration of antigen. Control samples containing a non-specific
IgG antibody were also studied and calibration curves obtained by subtraction of
the responses for specific and non-specific antibody-based sensors, thereby
eliminating the effects of non-specific adsorption of antigen.|
|Appears in Collections:||Staff publications - Cranfield Health|
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