Olufeagba, S.O. and Aluko, P.O. and Omotosho, J.S. and Oyewole, S.O. and Raji, A.
Induced diploid meiogynogenesis in the African clariid catfish Heterobranchus longifilis (Valenciennes, 1840) (Pisces: Clariidae).
In: 14th Annual Conference of the Fisheries Society of Nigeria (FISON)
, 19-23 January 1998
Diploid meiotic gynogenesis was induced in African catfish, Heterobranchus longifilis by injection of 0.5ml/kg ovaprim on the breeders, followed by application of UV light irradiation on the spermatozoa and temperature shocking of activated eggs. Diploidy was restored by shocking haploid activated eggs at 5 degree C for 40 minutes. The normal control spermatozoa did not receive any UV irradiation nor temperature shock, while the haploid control spermatozoa were irradiated, but did not receive cold shock. The percentage hatchability in the treated group was 25%, while in the control it was 53%. Less than 15 fingerlings had morphological aberrations. After two weeks of indoor rearing, the survival percentage of the treated group was 45% in the control experiment. Cytogenetic analysis of chromosomes revealed 25 chromosomes in the haploid embryo and 50 chromosomes each in diploid gynogenesis and normal diploid control
Conference or Workshop Item
||Induced diploid meiogynogenesis in the African clariid catfish Heterobranchus longifilis (Valenciennes, 1840) (Pisces: Clariidae)
||14th Annual Conference of the Fisheries Society of Nigeria (FISON)
||19-23 January 1998
||Fisheries Society of Nigeria
||Nigeria; aquaculture; aquaculture techniques; biotechnology; fish culture; induced breeding; Heterobranchus longifilis
Ms Maria Kalentsits
||11 Jul 2010 09:49
||29 Sep 2011 17:00
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