Figure 2: Migration of macrophages at 10 (a) and 30 mM (b) glucose, by in vitro scratch assay. (c): number of migrating cells (referred before). Cells were plated at 4 × 105/well and treated with 10 nM NT or 1 μM/mL LPS or both, during 24 h. The scratch assay was performed as described in “Material and Methods.” The images were acquired by transmission microscopy, and photographs were taken before cell treatment (0 h) and 24 h after treatments. Magnification used was 40x. Results are presented as mean ± SEM of three independent experiments. ; .