Disaggregation of micro-algae
Posted 14 November 2011 - 08:57 AM
I am currently helping one of our phd students with the development of a bio assay, we need to disaggregate some micro-algae into single cells but wish to keep the cells intact, plants are not an area of expertise for me, u have tried mechanical and ultrasonic disruption but these have had limited success.
Can anyone suggest a method for disaggregating the cells ?
Posted 14 November 2011 - 01:40 PM
If you know what is linking them (cellulose?) you could perhaps use an enzyme digest.
Edited by bob1, 14 November 2011 - 01:40 PM.