Affiliation: Fisheries and Aquaculture Science
- Detection of environmental androgens: a novel method based on enzyme-linked immunosorbent assay of spiggin, the stickleback (Gasterosteus aculeatus) glue proteinIoanna Katsiadaki
Centre for Environment, Fisheries and Aquaculture Science, The Nothe, Weymouth, Dorset, United Kingdom
Environ Toxicol Chem 21:1946-54. 2002..Possession of an androgen-regulated protein by the female stickleback makes it a unique bioassay organism for detecting androgenic contamination in the aquatic environment...
- The potential of the three-spined stickleback (Gasterosteus aculeatus L.) as a combined biomarker for oestrogens and androgens in European watersI Katsiadaki
CEFAS Weymouth Laboratory, Dorset, UK
Mar Environ Res 54:725-8. 2002....
- Use of the three-spined stickleback (Gasterosteus aculeatus) as a sensitive in vivo test for detection of environmental antiandrogensIoanna Katsiadaki
Centre for Environment, Fisheries and Aquaculture Science Cefas, Weymouth, Dorset, United Kingdom
Environ Health Perspect 114:115-21. 2006..Furthermore, the species is endemic and ubiquitous in Europe, and it possesses many ecological traits that make it better suited than other potential species for field research into endocrine disruption...
- Ethoxyresorufin-O-deethylase (EROD) and vitellogenin (VTG) in flounder (Platichthys flesus): system interaction, crosstalk and implications for monitoringM F Kirby
CEFAS, Burnham Laboratory, Remembrance Avenue, Burnham on Crouch, Essex CM0 8HA, United Kingdom
Aquat Toxicol 81:233-44. 2007..A greater understanding of system interaction and other factors, including genetics, that influence biomarker response to contaminants would be required to interpret biomarker monitoring data...
- Differential sensitivity of flounder (Platichthys flesus) in response to oestrogenic chemical exposure: an issue for design and interpretation of monitoring and research programmesM F Kirby
CEFAS, Burnham Laboratory, Remembrance Avenue, Burnham on Crouch, Essex, UK
Mar Environ Res 62:315-25. 2006..The findings of the study are discussed in terms of implications for further research into the differential response issue and how the initial plasma VTG figures contribute to a time-series from the Mersey, Tyne and Alde estuaries...
- Further refinement of the non-invasive procedure for measuring steroid production in the male three-spined stickleback Gasterosteus aculeatusM Sebire
Centre for Environment, Fisheries and Aquaculture Science, Barrack Road, The Nothe, Weymouth, DT4 8UB, UK
J Fish Biol 75:2082-94. 2009....
- Vitellogenin in the blood plasma of male cod (Gadus morhua): a sign of oestrogenic endocrine disruption in the open sea?Alexander P Scott
Centre for Environment, Fisheries and Aquaculture Science Cefas, Weymouth Laboratory, Barrack Road, Weymouth, Dorset, DT4 8UB, UK
Mar Environ Res 61:149-70. 2006..The possibility is suggested that large cod pick up oestrogenic endocrine disrupters through the food chain...
- Intercalibration exercise using a stickleback endocrine disrupter screening assayYvonne T Allen
Centre for Environment, Fisheries, and Aquaculture Science, Burnham Laboratory, Burnham on Crouch, Essex, UK
Environ Toxicol Chem 27:404-12. 2008..Further work is underway to assess the relevance and suitability of the screen for weakly acting estrogens, anti-androgens, and aromatase inhibitors...
- Biomarker responses in wild three-spined stickleback (Gasterosteus aculeatus L.) as a useful tool for freshwater biomonitoring: a multiparametric approachWilfried Sanchez
Institut National de l Environnement Industriel et des Risques, Unité d Evaluation des Risques Ecotoxicologiques, BP2, 60550, Verneuil en Halatte, France
Environ Int 34:490-8. 2008..This study argues for a multi-parametric approach of aquatic ecosystem contamination based on association between chemical, biochemical and ecological endpoints and provides a testimony of the usefulness of stickleback for this purpose...
- Three-spined stickleback: an emerging model in environmental endocrine disruptionIoanna Katsiadaki
CEFAS Weymouth Laboratory, Barrack Road, Weymouth, Dorset DT4 8UB, UK
Environ Sci 14:263-83. 2007..In European waters, the stickleback is the only fish that can bring laboratory and field studies together and allow the true impact of endocrine disruptors on fish populations to be evaluated...
- Detection and assessment of androgenic potency of endocrine-disrupting chemicals using three-spined stickleback, Gasterosteus aculeatusMasaki Nagae
Faculty of Environmental Studies, Nagasaki University, 1 14 Bunkyo machi, Nagasaki, Nagasaki 852 8521, Japan
Environ Sci 14:255-61. 2007..The quantification system for spiggin mRNA was established using a real-time RT-PCR technique. This system enables accurate quantification within a wide range of spiggin mRNA from 10(1) to 10(6) copies...
- Survey of estrogenic and androgenic disruption in Swedish coastal waters by the analysis of bile fluid from perch and biomarkers in the three-spined sticklebackMaria Pettersson
Department of Applied Environmental Science ITM, Stockholm University, SE 106 91 Stockholm, Sweden
Mar Pollut Bull 54:1868-80. 2007..Taken together, the results did not indicate estrogenic or androgenic disruption in the investigated waters...
- Global genomic methylation levels in the liver and gonads of the three-spine stickleback (Gasterosteus aculeatus) after exposure to hexabromocyclododecane and 17-beta oestradiolStanley O Aniagu
Molecular Pathobiology Group, School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
Environ Int 34:310-7. 2008..The contrasting effects of HBCD and E(2) on global DNA methylation in stickleback should contribute to the integrated risk assessment of these environmental chemicals...
- Effects of 17alpha-ethynylestradiol on EROD activity, spiggin and vitellogenin in three-spined stickleback (Gasterosteus aculeatus)Carin Andersson
Department of Environmental Toxicology, Uppsala University, Norbyvagen 18A, SE 75236 Uppsala, Sweden
Aquat Toxicol 83:33-42. 2007..These findings are important for interpretation of biomonitoring data...
- Non-invasive measurement of 11-ketotestosterone, cortisol and androstenedione in male three-spined stickleback (Gasterosteus aculeatus)Marion Sebire
The Centre for Environment, Fisheries and Aquaculture Science, Weymouth, Dorset DT4 8UB, UK
Gen Comp Endocrinol 152:30-8. 2007..The procedure is potentially a powerful tool for the study of the link between steroids and behaviour in this useful sentinel species...
- The juvenile three-spined stickleback (Gasterosteus aculeatus L.) as a model organism for endocrine disruption II--kidney hypertrophy, vitellogenin and spiggin inductionEdda Hahlbeck
Institute of Applied Environmental Research, Stockholm University, SE 106 91 Stockholm, Sweden
Aquat Toxicol 70:311-26. 2004..The tested endpoints are suitable for the study of endocrine disruption in juvenile sticklebacks, a fish species that is easy to handle in laboratory and relevant for temperate geographical regions...
- Relationship between sex steroid and vitellogenin concentrations in flounder (Platichthys flesus) sampled from an estuary contaminated with estrogenic endocrine-disrupting compoundsAlexander P Scott
Centre for Environment, Fisheries and Aquaculture Science Cefas, Weymouth, United Kingdom
Environ Health Perspect 114:27-31. 2006..However, matched by month of capture and stage of maturation, both 17beta-estradiol in females and 11-keto-testosterone in males were in most cases significantly lower in those years when VTG concentrations were higher...
- Surveys of plasma vitellogenin and intersex in male flounder (Platichthys flesus) as measures of endocrine disruption by estrogenic contamination in United Kingdom estuaries: temporal trends, 1996 to 2001Mark F Kirby
Centre for Environment, Fisheries and Aquaculture Science Burnham Laboratory, Remembrance Avenue, Burnham on Crouch, Essex CM0 8HA, United Kingdom
Environ Toxicol Chem 23:748-58. 2004..The significance of the findings and possible mitigating influences are discussed in terms of the impacts on wild fish and the role of effluent treatment in reducing these...
- Development of a stickleback kidney cell culture assay for the screening of androgenic and anti-androgenic endocrine disruptersCecile Jolly
Department of Biology, University of Bergen, Institute of Biology, HIB, Thormohlensgt 55, 5020 Bergen, Norway
Aquat Toxicol 79:158-66. 2006..This cell culture system provides an innovative tool for the rapid and sensitive screening of androgenic and anti-androgenic properties of environmental contaminants...
- Kinetics of vitellogenin protein and mRNA induction and depuration in fish following laboratory and environmental exposure to oestrogensJohn A Craft
School of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, Scotland, UK
Mar Environ Res 58:419-23. 2004..Low level increases of VTG mRNA (5 X) is observed in some fish without increased vitellogenin and this may represent polymorphic differences between individual fish...
- The impact of oestrogenic and androgenic contamination on marine organisms in the United Kingdom--summary of the EDMAR programme. Endocrine Disruption in the Marine EnvironmentPeter Matthiessen
Centre for Ecology and Hydrology, Ambleside, Cumbria, UK
Mar Environ Res 54:645-9. 2002..The majority of the oestrogenic activity is adsorbed to sediments, and routes of exposure may include benthic food chain transfer. Some natural androgenic substances are also being discharged to estuaries, but their activity appears low...
- Evidence for estrogenic endocrine disruption in an offshore flatfish, the dab (Limanda limanda L.)Alexander P Scott
Centre for Environment, Fisheries and Aquaculture Science Cefas, Barrack Road, Weymouth, Dorset DT4 8UB, UK
Mar Environ Res 64:128-48. 2007..However, our data, showing that larger fish are more likely to have elevated VTG concentrations, suggests a gradual accumulation by marine fish, probably through feeding, of persistent (probably relatively weak) estrogenic compounds...
- Construction of subtracted EST and normalised cDNA libraries from liver of chemical-exposed three-spined stickleback (Gasterosteus aculeatus) containing pollutant-responsive genes as a resource for transcriptome analysisMargaret M Brown
Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow, UK
Mar Environ Res 66:127-30. 2008..2-10-fold for transcripts expected to be induced in response to the pollutants...
- Exposure of sticklebacks (Gasterosteus aculeatus) to cadmium sulfide nanoparticles: biological effects and the importance of experimental designMatthew B Sanders
CEFAS Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset, UK
Mar Environ Res 66:161-3. 2008..The results are discussed emphasising the fundamental importance of experimental design and the need to understand the behaviour of nanoparticles in the aqueous phase...