Friederich-Persson 2013 Abstract MiP2013
|Friederich-Persson M, Hansell P, Wilcox CS, Palm F (2013) Potassium controls rat mitochondrial function: In vivo and in vitro considerations. Mitochondr Physiol Network 18.08.|
The intracellular potassium concentration, [K+], varies depending on tissue type and is about 60 mM in kidney proximal tubular cells whereas it is approximately 120 mM in cardiac myocytes. Intracellular [K+] may therefore affect mitochondria function.
By measuring the respiratory function and swelling of mitochondria isolated from kidney cortex and heart at [K+] ranging from 15 mM to 146 mM we showed that [K+] controls mitochondria function in the heart. Increasing [K+] resulted in increased resting state respiration and decreased respiratory control ratio (RCR) in kidney cortex mitochondria. The reduced RCR corresponded with mitochondria swelling, indicating failing mitochondria. The effects of K+ in kidney mitochondria was prevented by inhibitors of ATP-sensitive K+-channels and voltage-gated K+-channels (glibenclamide and by 4-aminopyridine, respectively). On the contrary, RCR in heart mitochondria increased with increasing [K+] and was unaffected by K+channel inhibitors.
These results demonstrate [K+] control of mitochondria function in rat kidney and heart.
• MiPNetLab: SE Uppsala Liss P
Labels: MiParea: Respiration, mt-Structure;fission;fusion Mammal;model: Rat Tissue;cell: Heart, Kidney Preparation: Isolated mitochondria Regulation: Flux control, Ion;substrate transport HRR: Oxygraph-2k Additional: MiP2013, S06
1 - Dept of Medical Cell Biology, Uppsala University, Sweden
2 - Dept of Medicine, Division of Hypertension and Nephrology, Georgetown University Medical Center, Washington DC, USA;
3 - Dept of Medical and Health Sciences, Linköping University, Sweden;
4 - Center for Medical Image Science and Visualization, Linköping University, Sweden.