Haas, Jürgen G.; Thiel, Corinna; Blömer, Kathi; Weiss, Elisabeth H.; Riethmüller, Gert; Ziegler-Heitbrock, H. W. Löms
Downregulation of Tumor Necrosis Factor Expression in the Human Mono-Mac-6 Cell Line.
In: Journal of Leucocyte Biology, Vol. 46: pp. 11-14
Mono-Mac-6 cells, but not U937 cells, can be Induced to rapidly express tumor necrosis
factor (TNF) mRNA and protein when triggered with Ilpopolysaccharlde (LPS) at 1 pg/mI.
Preincubatlon of the cells for 3 d with low amounts of LPS (10 ng/mI) results In nearly
complete suppression of TNF secretion. This downreguiatlon appears to occur at the
pretranslational level since specIfIc mRNA is virtually undetectable under these conditions.
By contrast, the same prelncubatlon with 10 ng/mI LPS results in enhanced phagocytosls
(28.6-67.2% for Staphylococcus aureus), demonstrating that not all monocyte
functions are suppressed. While these results show that only stringent exclusion of LPS
from culture media allows for Induction of TNF In the Mono-Mac-6 cell line, the pronounced
effect of LPS preincubatlon may also provide a suitable model with which to
study the mechanisms of LPS-lnduced desensitizatIon.