Extraintestinal pathogenic Escherichia coli in poultry meat products on the Finnish retail market
© Lyhs et al.; licensee BioMed Central Ltd. 2012
Received: 10 June 2012
Accepted: 13 November 2012
Published: 16 November 2012
Extraintestinal pathogenic Escherichia coli bacteria (ExPEC) exist as commensals in the human intestines and can infect extraintestinal sites and cause septicemia. The transfer of ExPEC from poultry to humans and the role of poultry meat as a source of ExPEC in human disease have been discussed previously. The aim of the present study was to provide insight into the properties of ExPEC in poultry meat products on the Finnish retail market with special attention to their prevalence, virulence and phylogenetic profiles. Furthermore, the isolates were screened for possible ESBL producers and their resistance to nalidixic acid and ciprofloxacin was tested.
The presence of ExPEC in 219 marinated and non-marinated raw poultry meat products from retail shops has been analyzed. One E. coli strain per product was analyzed further for phylogenetic groups and possession of ten virulence genes associated with ExPEC bacteria (kpsMT K1, ibeA, astA, iss, irp2, papC, iucD, tsh, vat and cva/cv) using PCR methods. The E. coli strains were also screened phenotypically for the production of extended-spectrum β-lactamase (ESBL) and the susceptibility of 48 potential ExPEC isolates for nalidixic acid and ciprofloxacin was tested.
E. coli was isolated from 207 (94.5%) of 219 poultry meat products. The most common phylogenetic groups were D (50.7%), A (37.7%), and B2 (7.7%). Based on virulence factor gene PCR, 23.2% of the strains were classified as ExPEC. Two ExPEC strains (1%) belonged to [O1] B2 svg+ (specific for virulent subgroup) group, which has been implicated in multiple forms of ExPEC disease. None of the ExPEC strains was resistant to ciprofloxacin or cephalosporins. One isolate (2.1%) showed resistance to nalidixic acid.
Potential ExPEC bacteria were found in 22% of marinated and non-marinated poultry meat products on the Finnish retail market and 0.9% were contaminated with E. coli [O1] B2 svg+ group. Marinades did not have an effect on the survival of ExPEC as strains from marinated and non-marinated meat products were equally often classified as ExPEC. Poultry meat products on the Finnish retail market may have zoonotic potential.
KeywordsExtraintestinal pathogenic Escherichia coli ExPEC Poultry meat Retail market Antibiotic resistance
Escherichia coli strains can be classified into three major groups: commensal strains, intestinal pathogenic strains, and extraintestinal pathogenic E. coli (ExPEC) strains. ExPEC strains derive predominantly from E. coli phylogenetic group B2, and to a lesser extent D, and were defined by Johnson et al. as E. coli isolates containing two or more of the following virulence markers: papA (P fimbriae structural subunit) and/or papC (P fimbriae assembly), sfa/foc (S and F1C fimbriae subunits), afa/dr (Dr-antigen-binding adhesins), kpsMT II (group 2 capsular polysaccharide units), and iutA (aerobactin receptor). ExPEC exist as commensals in the human intestines and can infect extraintestinal sites, such as the urinary tract, the meninges, the peritoneal cavity or lungs, and can also cause septicemia[3, 4]. The transfer of ExPEC from poultry to humans and the role of poultry meat as a source of ExPEC human disease have been discussed previously. Studies by Johnson et al.[2, 5–7] and Manges et al. indicated that retail meat products are frequently contaminated with E. coli, and that poultry and pork meat may be a potential source of ExPEC strains.
There is a wide variety of poultry products available on the Finnish retail market, including fresh and modified-atmosphere-packaged (MAP) products with or without spices. Most of these products, approximately 80%, are sold marinated with salt and water- and/or oil-based, spiced sauces. To the authors’ knowledge, there is no information about the prevalence of ExPEC in marinated and non-marinated poultry meat products on the Finnish retail market.
The use of antibiotic agents in food animal production has raised concern about the transmission of antibiotic-resistant bacteria to humans via the food supply. Acquired resistance to first-line antimicrobial agents has been found to increasingly complicate the management of ExPEC infections in humans. Resistance to fluoroquinolones and third- and fourth-generation cephalosporins is increasing in many countries[9–12]. Increasing numbers of extended-spectrum β-lactamase (ESBL)-producers are seen in Finnish uro-pathogenic E. coli (UPECs) (M. Vaara, personal communication). ESBL producing E. coli strains have emerged as a potential health hazard, as they have not only been identified from human clinical samples, but also from food producing animals[10, 13].
The aim of the present study was to provide insight into the properties of ExPEC in poultry meat products on the Finnish retail market in order to evaluate their zoonotic potential. Special attention was given to their prevalence and the virulence and phylogenetic profiles. Furthermore, the isolates were screened for possible ESBL producers and their antibiotic resistance to nalidixic acid and ciprofloxacin was tested.
Materials and methods
Product description and sampling
A total of 219 retail raw chilled poultry meat products from different batches (199 chicken samples, 16 turkey samples and four samples including both chicken and turkey meat) were randomly selected between July 2006 and September 2006 from different local retail shops in Western Finland (88 samples) and between August 2006 and April 2007 in the Helsinki area (131 samples). Of all samples, 178 products were marinated and 41 were non-marinated. All products were packed in Finland, but in 31 (14.2%) samples the meat originated abroad. The term “marinated” refers to products with an oil- and/or water-based marinade and a blend of spices. All products were packed under a modified atmosphere consisting of carbon dioxide and nitrogen in different proportions, and had a shelf life of up to 10 days. All samples were transported immediately to the laboratory and kept at 4 ± 3°C until being analyzed within 24 hours of purchase.
Culture method and identification
Each sample was aseptically removed from the package and placed in a Stomacher bag (Seward BA6041, Worthing, UK). Equal amounts of a weighed sample and Buffered Peptone Water (BPW) (LAB46, LabM, Lancashire, UK) were mixed with a minimal amount of 300 g of meat in 300 ml of BPW (LabM). The bag was shaken manually for 3 minutes and100 μl of BPW was spread onto Chromocult® Coliform Agar (CCA) (Merck, Darmstadt, Germany) plates. All plates were incubated for 24 hours at 37°C. One presumptive E. coli colony isolated from each product was subcultured on Tryptic Soy Agar (TSA) agar (Merck, Darmstadt, Germany), and incubated as described above. For further identification, API 20E (bio Mérieux, Marcy l`Etoile, France) was used.
A total of 207 E. coli isolates were characterized for their virulence factor genes using the polymerase chain reaction (PCR) method. Ten virulence genes were studied: kpsMT K1, ibeA, astA, iss, irp2, papC, iucD, tsh, vat, and cva/cvi. The single PCR reactions for kpsMT K1 and ibeA were performed as described by Johnson et al.. The presence of eight virulence factor genes - astA, iss, irp2, papC, iucD, tsh, vat, and cva/cvi was studied using the multiplex PCR method by Ewers et al.. All amplifications were performed in a 25 μl reaction volume, and a lysate of overnight culture in sterile water was used as a template.
Phylogenetic and O typing
E. coli isolates were assigned to phylogenetic groups using the PCR method documented earlier[16, 17]. Based on the combination of genes (svg, chuA, yjaA, uidA and TspE4.C2) they possess, the isolates were assigned to one of five groups (A, B1, B2, D, B21/ST29). E. coli O antigen groups O1, O2 and O18 were determined according to Clermont et al.. Amplifications were performed in a 25 μl reaction volume, and a lysate of overnight culture in sterile water was used as a template.
The following E. coli strains were used as positive PCR controls: RS 228 and A56 (K1, O18 ibeA); RS 228 (positive for all five genes), and ATCC 25922 (positive for all five genes except svg) for the phylogenetic groups; A110 (astA, irp2, papC); EC206 (iss, irp2, papC, iucD, tsh, cva); 252 (iss, irp2, papC, iucD, vat, cva, O2); IH11002 (O1).
Antimicrobial susceptibility testing
The susceptibility of 48 potential ExPEC isolates for nalidixic acid (30 μg) and ciprofloxacin (5 μg) was tested according to the standard Disk Susceptibility method. The E. coli strains were also screened phenotypically for the production of ESBL with the double disk synergy test. Disks (Oxoid) containing cefotaxime, ceftazidime, and equivalent disks with clavulanic acid were used according to the standard method.
Comparisons of proportions for virulence factors detected and the distribution of the strains to phylogenetic groups were analyzed by Chi-square test. Statistical significance was set at a P value of < 0.001.
Phylogenetic distribution of the 207 Escherichia coli isolates originating from marinated and non-marinated retail poultry meat at Finnish retail market
Non-marinated meat (n = 40)
Marinated meat (n = 167)
Total (n = 207)
uidA negative ns
Distribution of virulence factor genes (%) among 207 Escherichia coli –isolates belonging to major phylogenetic groups and isolated from poultry meat at Finnish retail market
Virulence factor genes
Prevalence of trait within phylogenetic groups, no. (%)
Total (n = 207)
A + B1 (+uidA neg.) (n = 84)
D (n = 105)
B2 (+B21/ST29) (n = 18)
Virulence gene profiles and phylogeny groups in ExPEC isolates ( n = 48) and their distribution in marinated and non-marinated poultry meat at Finnish retail market
Combinations of genes
Non-marinated meat (n = 40)
Marinated meat (n = 167)
cva, vat, iucD, papC, irp2, iss, kpsMt K1
cva, vat, tsh, iucD, irp2, iss, ibeA, kpsMT K1
cva, vat, tsh, iucD, papC, iss, astA
cva, iucD, irp2, iss, astA, kpsMt K1
cva, iucD, irp2, astA, kpsMt K1
vat, iucD, papC, irp2, iss, astA, kpsMT K1
iucD, papC, irp2, iss, astA, kpsMT K1
iucD, irp2, iss, astA, kpsMT K1
iucD, irp2, astA, kpsMT K1
iucD, iss, astA, kpsMT K1
iucC, papC, irp2, iss
iucC, irp2, kpsMT K1
B2 and D
The 48 isolates classified as ExPEC were susceptible to cephalosporins, ciprofloxacin and nalidixic acid, with the exception of one isolate (1/48; 2.1%) showing resistance to nalidixic acid. This ExPEC strain belonged to phylogenetic group D, and was positive for seven of the ten virulence factors studied.
In the present study, almost all Finnish retail poultry meat products were found to be contaminated with E. coli. Twenty-two per cent of the products contained E. coli strains that could be classified as ExPEC. The analysis of these poultry meat samples was focused to the virulence genes that are most typical of avian pathogenic ExPEC, and thus most likely to prevail in poultry products. Inclusion of all kps MT II group genes, instead of only that for K1 capsule, and some other ExPEC associated virulence factor genes, could have increased the observed prevalence of ExPEC. The findings from the Finnish poultry products are consistent with studies made in other countries on poultry meat at the retail level. Johnson et al. found 21% of 110 E. coli isolates from chicken products to satisfy the criteria for ExPEC. Johnson et al. reported a contamination rate of 46% for ExPEC in 189 retail poultry products. Xia et al. found among different retail meats the highest rate of ExPEC strains in ground turkey (23.5%) and chicken breasts (20.2%). Kobayashi et al. isolated 80 strains from 57 retail chicken carcasses, and grouped 21.25% of the strains into group B2 and 28.75% into group D. It has been recently shown that B2 strains positive for svg belong to a subset of ExPEC strains that contain avian pathogenic, uropathogenic as well as neonatal meningitis causing E. coli. These strains often belong to serogroups O1, O2 or O18, and contain a high number of virulence genes. In this study, such ExPEC bacteria were found in 1% of Finnish poultry meat products. The isolates belonged to serogroup O1, and contained seven of the ten virulence genes studied.
Fecal contamination of carcasses at slaughter is presumed to be the source of contamination of meat with potential ExPEC bacteria[24–26]. However, our previous studies (unpublished) detected E. coli fulfilling ExPEC criteria only in 2.3% of fecal samples from healthy broilers. All these isolates belonged to phylogroup D, and none to B2. Our studies have shown that colibacillosis caused by avian pathogenic E. coli is not an uncommon disease in Finnish broiler houses. The study shows that such ExPEC bacteria can efficiently contaminate poultry meat and enter the food chain. In Finland, poultry meat is usually cut into pieces or very thin slices. It can be speculated that cutting may provide favorable or even selective conditions for the survival of ExPEC bacteria in the product consisting of meat and meat juice. The conditions may mimic those in the host tissue, thus supporting the survival of bacteria possessing protective virulence mechanisms. Meat processing may favor the survival of other types of E. coli as well, such ESBL-producing strains, in the meat products.
Marinades and especially their spices may potentially serve as sources of food pathogens, and furthermore promote pathogen survival. This has been a matter of concern in Finland, where most of the poultry meat is sold in marinades. In this study, we did not observe any differences between E. coli strains from marinated and non-marinated products with regard to prevalence of potential ExPEC and other virulence factor genes and phylogenetic groups. The studies on survival of E. coli in marinated meat products have focused on verocytotoxigenic strains. It has been stated that acidification of a traditional marinade resulted in greater lethality of E. coli O157:H7 compared with otherwise similar marinades that were not further acidified[27, 28]. The pH of commercial marinades used in Finnish retail poultry meat products ranges from pH 4.16 ± 0.03 to pH 4.5. It has been speculated that the buffering capacity of meat might neutralize the acidic components in marinades, leading to a decreased antimicrobial effect. Although this study did not indicate that marinades are a risk factor for ExPEC contamination of the product, it is necessary to study the effect of marinades on survival of zoonotic E. coli, such as ESBL-producing strains.
In the present study, all potential ExPEC isolates (n = 48) were susceptible to ciprofloxacin and cephalosporins, and only one isolate exhibited resistance to nalidixic acid. These results illustrate the very low level of antimicrobials used in poultry production in Finland. Meat producing flocks, in particular, are usually reared without any antimicrobial treatment. The percentage of flocks treated has varied between 0.0 and 0.37% in broiler flocks and 3.79–4.97% in turkey flocks during 2007–2010 (http://www.ett.fi). No fluoroquinolones or cephalosporins are used for poultry. Cephalosporin resistant E. coli isolates have emerged in numerous locations around the world after the sampling time of this study. This study, covering the Finnish retail market in 2006–2007, serves as a starting point for further studies. It is essential to gain more information about the antibiotic resistance and the genetic properties of the E. coli isolates present in retail poultry meat in Finland.
Potential ExPEC bacteria were found in 22% of marinated and non-marinated poultry meat products on the Finnish retail market. Of the products, 0.9% were contaminated with E. coli [O1] B2 svg+ group, which has been implicated in multiple forms of ExPEC disease. There were no differences between marinated and non-marinated products with regard to the prevalence of potential ExPEC bacteria, other virulence factor genes and phylogenetic groups of the E. coli isolates. Poultry meat products on Finnish retail market may have zoonotic potential.
The authors wish to thank Marianne Katzav for her excellent assistance. The practical assistance from the staff of Finnish Food Safety Authority Evira, Seinäjoki Research Unit is gratefully acknowledged.
- Russo TA, Johnson JR: Proposal for a new inclusive designation for extraintestinal pathogenic isolates of Escherichia coli: ExPEC. J Infect Dis. 2000, 181: 1753-1754. 10.1086/315418.View ArticlePubMed
- Johnson JR, Murray AC, Gajewski A, Sullivan M, Snippes P, Kuskowski MA, Smith KE: Isolation and molecular characterization of nalidixic acid-resistant extraintestinal pathogenic Escherichia coli from retail chicken products. Antimicrob Agents Chemother. 2003, 47: 2161-2168. 10.1128/AAC.47.7.2161-2168.2003.PubMed CentralView ArticlePubMed
- Manges AR, Smith SP, Lau BJ, Nuval CJ, Eisenberg JNS, Dietrich PS, Riley LW: Retail meat consumption and the acquisition of antimicrobial resistant Escherichia coli causing urinary tract infections: a case–control study. Foodborne Pathog Dis. 2007, 4: 419-431. 10.1089/fpd.2007.0026.View ArticlePubMed
- Smith JL, Fratamico PM, Gunther NW: Extraintestinal pathogenic Escherichia coli. Foodborne Pathog Dis. 2007, 4: 134-163. 10.1089/fpd.2007.0087.View ArticlePubMed
- Johnson JR, Del Vari P, O'Bryan TT, Smith K, Tatini S: Contamination of retail foods, particularly turkey, from community markets (Minnesota, 1999–2000) with antimicrobial-resistant and extraintestinal pathogenic Escherichia coli. Foodborne Pathog Dis. 2005, 2: 38-49. 10.1089/fpd.2005.2.38.View ArticlePubMed
- Johnson JR, Kuskowski MA, Smith K, O'Bryan TT, Tatini S: Antimicrobial-resistant and extraintestinal pathogenic Escherichia coli in retail foods. J Infect Dis. 2005, 191: 1040-1049. 10.1086/428451.View ArticlePubMed
- Johnson TJ, Logue CM, Wannemuehler YM, Kariyawasam S, Doetkott C, Debroy C, White DG, Nolan LK: Examination of the source and extended virulence genotypes of Escherichia coli contaminating retail poultry meat. Foodborne Pathog Dis. 2009, 6: 657-667. 10.1089/fpd.2009.0266.PubMed CentralView ArticlePubMed
- Björkroth J: Microbiological ecology of marinated meat products. Meat Sci. 2005, 70: 477-480. 10.1016/j.meatsci.2004.07.018.View ArticlePubMed
- Taylor NM, Davies RH, Ridley A, Clouting C, Wales AD, Clifton-Hadley FA: A survey of fluoroquinolone resistance in Escherichia coli and thermophilic Campylobacter spp. on poultry and pig farms in Great Britain. J Appl Microbiol. 2008, 105: 1421-1431. 10.1111/j.1365-2672.2008.03877.x.View ArticlePubMed
- Costa D, Vinue L, Poeta P, Coelho AC, Matos M, Saenz Y, Somalo S, Zarazaga M, Rodrigues J, Torres C: Prevalence of extended-spectrum beta-lactamase-producing Escherichia coli isolates in faecal samples of broilers. Vet Microbiol. 2009, 138: 339-344. 10.1016/j.vetmic.2009.03.029.View ArticlePubMed
- Cortés P, Blanc V, Mora A, Dahbi G, Blanco JE, Blanco M, López C, Andreu A, Navarro F, Alonso MP, Bou G, Blanco J, Llagostera M: Isolation and characterization of potentially pathogenic antimicrobial-resistant Escherichia coli strains from chicken and pig farms in Spain. Appl Environ Microbiol. 2010, 76: 2799-2805. 10.1128/AEM.02421-09.PubMed CentralView ArticlePubMed
- Randall LP, Clouting C, Horton RA, Coldham NG, Wu G, Clifton-Hadley FA, Davies RH, Teale CJ: Prevalence of Escherichia coli carrying extended-spectrum β-lactamases (CTX-M and TEM-52) from broiler chickens and turkeys in Great Britain between 2006 and 2009. J Antimicrob Chemother. 2011, 66: 86-95. 10.1093/jac/dkq396.View ArticlePubMed
- Smet A, Martel A, Persoons D, Dewulf J, Heyndrickx M, Catry B, Herman L, Haesebrouck F, Butaye P: Diversity of extended-spectrum beta-lactamases and class C beta-lactamases among cloacal Escherichia coli isolates in Belgian broiler farms. Antimicrob Agents Chemother. 2008, 52: 1238-1243. 10.1128/AAC.01285-07.PubMed CentralView ArticlePubMed
- Johnson JR, Stell AL: Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise. J Infect Dis. 2000, 181: 261-272. 10.1086/315217.View ArticlePubMed
- Ewers C, Janssen T, Kiessling S, Philipp HC, Wieler LH: Rapid detection of virulence-associated genes in avian pathogenic Escherichia coli by multiplex polymerase chain reaction. Avian Dis. 2005, 49: 269-273. 10.1637/7293-102604R.View ArticlePubMed
- Clermont O, Bonacorsi S, Bingen E: Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol. 2000, 66: 4555-4558. 10.1128/AEM.66.10.4555-4558.2000.PubMed CentralView ArticlePubMed
- Bidet P, Metais A, Mahjoub-Messai F, Durand L, Dehem M, Aujard Y, Bingen E, Nassif X, Bonacorsi S: Detection and identification by PCR of a highly virulent phylogenetic subgroup among extraintestinal pathogenic Escherichia coli B2 strains. Appl Environ Microbiol. 2007, 73: 2373-2377. 10.1128/AEM.02341-06.PubMed CentralView ArticlePubMed
- Clermont O, Johnson JR, Menard M, Denamur E: Determination of Escherichia coli O types by allele-specific polymerase reaction: application to the O types involved in human septicemia. Diagn Microbiol Infect Dis. 2007, 57: 129-136. 10.1016/j.diagmicrobio.2006.08.007.View ArticlePubMed
- Clinical and Laboratory standards institute: Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; Approved standard – Third edition. CLSI document M31-A3. 28. 2008, Wayne, PA, USA: Clinical and Laboratory standards institute, Ref Type: Generic
- Clinical and Laboratory standards institute: Performance standards for antimicrobial susceptibility testing; sixteenth informational supplement. CLSI document M100-S16. 26. 2006, Wayne, PA, USA: Clinical and Laboratory standards institute, Ref Type: Generic
- Xia X, Meng J, Zhao S, Bodeis-Jones S, Gaines SA, Ayers SL, McDermott PF: Identification and antimicrobial resistance of extraintestinal pathogenic Escherichia coli from retail meats. J Food Prot. 2011, 74: 38-44. 10.4315/0362-028X.JFP-10-251.View ArticlePubMed
- Kobayashi RKT, Aquino I, Ferreira ALS, Vidotto MC: EcoR phylogenetic analysis and virulence genotyping of avian pathogenic Escherichia coli strains and Escherichia coli isolates from commercial chicken carcasses in Southern Brazil. Foodborne Pathog Dis. 2011, 8: 631-634. 10.1089/fpd.2010.0726.View ArticlePubMed
- Johnson TJ, Wannemuehler Y, Johnson SJ, Stell AL, Doetkott C, Johnson JR, Kim KS, Spanjaard L, Nolan LK: Comparison of extraintestinal pathogenic Escherichia coli strains from human and avian sources reveals a mixed subset representing potential zoonotic pathogens. Appl Environ Microbiol. 2008, 74: 7043-7050. 10.1128/AEM.01395-08.PubMed CentralView ArticlePubMed
- Altekruse SF, Elvinger F, DebRoy C, Pierson FW, Eifert JD, Sriranganathan N: Pathogenic and Fecal Escherichia coli Strains from Turkeys in a Commercial Operation. Avian Dis. 2002, 46: 562-569. 10.1637/0005-2086(2002)046[0562:PAFECS]2.0.CO;2.View ArticlePubMed
- de Brito BG, Carlos L, Gaziri J, Vidotto MC: Virulence factors and clonal relationships among Escherichia coli strains isolated from broiler chickens with cellulitis. Infect Immun. 2003, 71: 4175-4177. 10.1128/IAI.71.7.4175-4177.2003.PubMed CentralView ArticlePubMed
- Rodriguez-Siek KE, Giddings CW, Doetkott C, Johnson TJ, Nolan LK: Characterizing the APEC pathotype. Vet Res. 2005, 36: 241-256. 10.1051/vetres:2004057. 241View ArticlePubMed
- Derrickson-Tharrington EL: Evaluation of common acidulants for enhancement of destruction of Escherichia coli O157:H7 during drying of Gala apple slices and whole muscle beef jerky. 2001, Fort Collins: Colorado State University, MS thesis
- Calicioglu M, Sofos JN, Samelis J, Kendall PA, Smith GC: Inactivation of acid-adapted and non-adapted Escherichia coli O157:H7 during drying and storage of beef jerky treated with different marinades. J Food Prot. 2002, 65: 1394-1405.PubMed
- Isohanni P, Alter T, Saris P, Lyhs U: Wines as possible meat marinade ingredients possess antimicrobial potential against Campylobacter. Poult Sci. 2010, 89: 2704-2710. 10.3382/ps.2009-00521.View ArticlePubMed
- Perko-Mäkelä P, Koljonen M, Miettinen M, Hänninen M-L: Survival of Campylobacter jejuni in marinated and non-marinated chicken products. J Food Saf. 2000, 20: 209-216. 10.1111/j.1745-4565.2000.tb00299.x.View Article
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