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The answer is by combining linkage analysis (as described in Chapter 5) with the use of DNA markers described earlier in this chapter to localize the human disease gene to a spe- cific region of chromosomal DNA. Schink B (1997) Energetics of syntrophic cooperation in methanogenic degradation.
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NPCs are constructed of roughly 100 differentproteinsandhaveamassofabout125MDa. The 145 mutation appears to be the most common. Application of molecular biological techniques has resulted in the availability of large amounts of protein, and recently determined protein 6 ENCYCLOPEDIA OF LIFE SCIENCES 2001 Nature Publishing Group www.
Cell 93 2535. (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity.
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