|4759932||Method of reducing heat stress in animals||1988-07-26||Laurent et al.||426/2|
|4610883||Method of improving liveability of poultry||1986-09-09||Laurent et al.||426/72|
|4610882||Zeolite in poultry feed utilization||1986-09-09||Laurent et al.||426/72|
|4556564||Feeding of zeolite A to poultry||1985-12-03||Laurent et al.||426/74|
|4537771||Antacids containing zeolitic molecular sieves||1985-08-27||Greb et al.||424/154|
|4529593||Use of gallium salts to treat disorders of calcium homeostasis||1985-07-16||Warrell, Jr. et al.||424/127|
|4515780||Animal nutrition||1985-05-07||Laurent et al.||424/154|
|4437329||Method of manufacturing twisted tubes||1984-03-20||Goldstein et al.||119/1|
|3776188||METHOD FOR INHIBITING THE FORMATION OF MALODORS FROM POULTRY FARMS||1973-12-04||Komakine||119/1|
|3370953||Method of producing a deodorizing feed||1968-02-27||Nakano||99/2|
|3120834||Method of introducing adjusvants into avian egg interiors||1964-02-11||Goldhaft et al.||119/1|
|EP0201804||1992-07-01||Composition to treating bone disorders.|
|EP0224856||1987-06-10||Bone disorder treatment.|
|JP59203450||November, 1984||FEED ADDITIVE|
This application is a continuation-in-part of the following four applications: Ser. No. 145,232, filed Jan. 19, 1988 now abandoned; Ser. No. 82,256, filed Aug. 6, 1987 now U.S. Pat. No. 4,820,526; Ser. No. 42,987, filed Apr. 27, 1987 now abandoned; and Ser. No. 934,461, filed Nov. 24, 1986 now abandoned. Application Ser. No. 145,232 is a continuation-in-part of application Ser. No. 42,987, which in turn is a continuation of application Ser. No. 846,188, filed Mar. 3, 1986 (now abandoned), which in turn is a continuation of application Ser. No. 741,572, filed June 5, 1985 (now abandoned), which in turn is a division of application Ser. No. 475,370 filed Mar. 14, 1983, now U.S. Pat. No. 4,556,564. Application Ser. No. 82,256, filed Aug. 6, 1987, is a continuation-in-part of each of the following four applications: Ser. No. 934,457, now U.S. Pat. No. 4,759,932; Ser. No. 934,460 now abandoned, Ser. No. 934,458 now abandoned, and Ser. No. 934,461 now abandoned, all filed on Nov. 24, 1986, each of which is a continuation-in-part of said application Ser. No. 846,188, filed Mar. 3, 1986, (now abandoned).
The present invention is in the general field of poultry farming and relates particularly to the feeding of laying fowl or layers. In a particular aspect, it relates to a method of feeding female birds with a feed formulation which enhances, for example, interior egg quality, as well as egg production, and/or egg shell quality. This invention also relates to the eggs with improved egg shells obtained from birds fed a diet of this invention. Poultry diets of this invention are typically diets which include a zeolite such as zeolite A in an amount such that degradation of the interior quality of eggs is retarded. This invention also relates to improved poultry farming. Thus, it relates to methods which provide improved egg production. These methods comprise obtaining an increase in yield of marketable eggs from laying hens. The increase is provided by feeding hens a diet of this invention.
The literature associated with poultry nutrition, and the effect of both nutrition and egg processing on egg quality is so vast as to defy brief summation. A few of the developments in this field are highlighted below.
In previously issued patents, we disclosed that zeolite A enhances egg shell quality, U.S. Pat. No. 4,556,564; improves feed utilization efficiency, U.S. Pat. No. 4,610,882; and improves liveability, U.S. Pat. No. 4,610,883. The disclosures in those patents are incorporated by reference herein as if fully set forth. Other related art is mentioned in the patents.
An article by C. Y. Chung et al from Nongsa Sihom Young Pogo 1978, 20 (Livestock) pp. 77-83 discusses the effects of cation exchange capacity and particle size of zeolites on the growth, feed efficiency and feed materials utilizability of broilers or broiling size chickens. Supplementing the feed of the broilers with naturally occurring zeolites, such as clinoptilolite, some increase in body weight gain was determined. Chung et al also reported that earlier results at the Livestock Experiment Station (1974, 1975, 1976--Suweon, Korea) showed that no significant difference was observed when 1.5, 3, and 4.5 percent zeolite was added to chicken layer diets.
U.S. Pat. No. 3,836,676 issued to Chukei Komakine in 1974 discloses the use of zeolites and ferrous sulfate crystals in an odorless chicken feed comprising such crystals and chicken droppings. The results were said to be no less than those in the case where chickens were raised with ordinary feed.
Experiments have been in progress in Japan since 1965 on the use of natural zeolite minerals as dietary supplements for poultry, swine and cattle. Significant increases in body weight per unit of feed consumed and in the general health of the animals was reported (Minato, Hideo, Koatsugasu 5:536, 1968). Reductions in malodor were also noted.
Using clinoptilolite and mordenite from northern Japan, Onagi, T. (Rept. Yamagata Stock Raising Inst. 7, 1966) found that Leghorn chickens required less food and water and gained as much weight in a two-week trial as birds receiving a control diet. No adverse effects on health or mortality were noted. The foregoing Japanese experiments were reported by F. A. Mumpton and P. H. Fishman in the Journal of Animal Science, Vol. 45, No. 5 (1977) pp. 1188-1203.
Canadian Pat. No. 939,186 issued to White et al in 1974 discloses the use of zeolites having exchangeable cations as a feed component in the feeding of urea or biuret non-protein (NPR) compounds to ruminants, such as cattle, sheep and goats. Natural and synthetic as well as crystalline and non-crystalline zeolites are disclosed. Zeolites tested included natural zeolites, chabazite and clinoptilolite and synthetic zeolites X, Y, F, J, M, Z, and A. Zeolite F was by far the most outstanding and zeolite A was substantially ineffective.
In a study at the University of Georgia, both broilers and layers were fed small amounts (about 2%) of clinoptilolite, a naturally occurring zeolite from Tilden, Tex. The egg shells from the hens receiving zeolite were slightly more flexible as measured by deformation, slightly less strong as measured by Instron breaking strength, and had a slightly lower specific gravity. The differences in egg shell quality were very small. This type of zeolite was ineffective in producing a stronger egg shell. An article written by Larry Vest and John Shutze entitled "The Influence of Feeding Zeolites to Poultry Under Field Conditions" summarizing the studies was presented at Zeo-Agriculture '82.
A study by H. S. Nakone of feeding White Leghorn layers clinoptilolite, reported in 1981 Poultry Science 60:944-949, disclosed no significant differences in egg shell strength between hens receiving the zeolite and hens not receiving the zeolite.
As is well known, the interior quality of eggs is diminished with time. The loss in quality attributes of the albumen and yoke is a function of temperature and movement of carbon dioxide through the shell. Low temperatures decrease the rate of loss in Haugh units--a standard measure of interior egg quality--and thus it is recommended that eggs be stored at temperatures close to the freezing point, a procedure which, as a practical matter, is not always feasible. To reduce rate of carbon dioxide (and moisture) loss various shell treatments have been utilized, such as spraying oil on the eggs.
A way has been discovered for reducing, if not eliminating, the need for refrigeration and spray oiling on eggs as a means of preserving interior egg quality.
To ameliorate the problem of interior egg degradation with time, the following procedures have been recommended heretofore:
1. Gather eggs three to four times per day.
2. Clean the eggs promptly after gathering and cool for 12-24 hours at 13° C. or preferably 10° C. before packing in cases or cartons.
3. Keep the eggs at between 60 to 85%, preferably 70 to 80%, relative humidity.
4. Resort to careful handling.
5. Use proper packing using precooled containers only.
6. Resort to frequent marketing of not less than twice a week.
7. Use speedy, refrigerated transportation and make frequent deliveries to sales outlets, preferably at least five times per week.
8. Use adequately refrigerated holding spaces at sales outlets.
9. Keep the eggs in home refrigerators at 7° C. to 13° C., and preferably use all of the eggs within one week.
The demand for poultry eggs, especially chicken eggs expanded considerably over the last decade. The poultry industry has grown from a home industry to a large scale manufacturing industry in which tens of thousands of eggs are produced daily at single farms or egg laying installations. Some eggs are produced for eating and some eggs are produced for hatching. One problem with such large scale egg producing is premature reduction in interior egg quality as a function of time. That is, unless eggs are handled and/or treated in accordance with the above 9-point program, their quality as regards internal qualities of the albumen and yolk may deteriorate faster than would be desired. Moreover, a way of simplifying egg production, distribution, marketing, etc. with concomittant reduction in refrigeration requirements would be a welcome contribution to the art. Another problem associated with large scale egg production is breakage. Even a slight crack in an egg makes it unsuitable for hatching and most other marketing purposes. It is estimated that some six percent of all eggs produced are lost for marketing because of cracking or breakage. Shell strength is very important to inhibit breakage. The stronger the egg shell, the less likely the egg will be cracked or broken. Machinery and techniques necessary for carefully handling the eggs to avoid breakage are expensive and time consuming.
Another substantial loss of egg production, estimated to be about a seven percent loss, is the production of shell-less eggs. Any reduction in shell-less eggs can be an important factor in large scale egg production.
We have discovered that internal egg quality can be maintained at a higher level by feeding laying poultry a diet containing zeolite A. In other words, this invention provides, in one of its embodiments, a process for reducing the rate of internal egg quality degradation which comprises feeding a laying poultry hen a diet which contains zeolite A such that the rate in decrease in Haugh unit value of the eggs from said hen is reduced as compared to the rate of such decrease under the same conditions with the same diet absent the zeolite A. In this process, the amount of zeolite A is usually within the range of about 0.25 to about 3.5 weight percent, preferably between about 0.75 to about 1.5 weight percent.
As stated above, our previously issued U.S. Pat. No. 4,556,564 discloses that zeolite A in poultry feed improves egg shell quality.
In Nutrient Requirements of Poultry, Eighth Revised Edition 1984, National Academy Press, Washington, D.C. (1984) it is stated in Table 4, (page 12) that leghorn-type chickens, both layers and breeders, have a dietary requirement of 0.15 weight % chlorine per day. Assuming an average daily intake per hen of 110 g of feed per day, this amounts to 165 mg of chlorine, per hen per day. This requirement is usually furnished as chloride ion, typically in salt, i.e. NaCl.
In the practice of this invention, the chloride level of the poultry diet may be reduced in order to improve egg yolk production and/or egg shell quality. At the same time, the utilization of such a diet achieves improved retention of internal egg quality as manifested by egg crops in which the rate in decrease in Haugh unit value of the eggs is reduced as compared to the rate of such decrease under the same conditions with the same diet absent the zeolite A.
Accordingly, we have discovered that egg production, egg quality, notably internal egg quality, and/or egg shell quality of poultry eggs is enhanced if, besides adding a zeolite (notably zeolite A) to the diet, the chloride level in poultry diets is decreased below the previously recommended requirement. The improvement can result in the production of more collectable eggs, which with broiler breeder hens means more eggs that are settable in incubators.
The enhancement of results provided by addition of zeolite A is unexpected. Furthermore, reduction of chloride levels below the previously required amount is unexpected.
This invention comprises a method for improving the internal quality of eggs in terms of preservation of Haugh units. This method involves, in another embodiment, a process for obtaining an egg crop having a reduced rate of internal egg quality degradation which comprises (i) feeding laying poultry a diet which contains from about 0.25 to about 3.0 weight percent zeolite A, and (ii) recovering an egg crop therefrom in which the rate in decrease in Haugh unit value of the eggs is reduced as compared to the rate of such decrease under the same conditions with the same diet absent the zeolite A. In addition, this invention comprises a method for improving eggs, egg production and/or egg shell quality. This method comprises feeding birds a diet which (a) contains reduced amounts of chlorine, e.g. less chloride than what was previously recognized as required, and which (b) also contains a zeolite such as zeolite A. Thus, in an important aspect, this invention in one of its forms can be considered a method of enhancing the quality of eggs, both in interior quality and in egg shell quality by use of a zeolite.
In still another preferred embodiment, this invention provides an egg produced by laying poultry fed a diet which contains zeolite A, the egg being characterized by exhibiting a rate of decrease in Haugh unit value that is lower than the rate of such decrease under the same conditions with the same diet absent the zeolite A. Preferably, these eggs are produced by a chicken hen, although the invention is deemed applicable to comparable improvements in duck eggs, quail eggs, turkey eggs, and the like.
Zeolites are crystalline, hydrated aluminosilicates of alkali and alkaline earth cations, having infinite, three-dimensional structures.
Zeolites consist basically of a three-dimensional framework of SiO4 and AlO4 tetrahedra. The tetrahedra are cross-linked by the sharing of oxygen atoms so that the ratio of oxygen atoms to the total of the aluminum and silicon atoms is equal to two or O/(Al+Si)=2. The electrovalence of each tetrahedra containing aluminum is balanced by the inclusion in the crystal of a cation, for example, a sodium ion. This balance may be expressed by the formula Al/Na=1. The spaces between the tetrahedra are occupied by water molecules prior to dehydration.
Zeolite A may be distinguished from other zeolites and silicates on the basis of their composition and X-ray powder diffraction patterns and certain physical characteristics. The X-ray patterns for these zeolites are described below. The composition and density are among the characteristics which have been found to be important in identifying these zeolites.
The basic formula for all crystalline sodium zeolites may be represented as follows:
Na2 O.Al2 O3.xSiO2.yH2 O
In general, a particular crystalline zeolite will have values for "x" and "y" that fall in a definite range. The value "x" for a particular zeolite will vary somewhat since the aluminum atoms and the silicon atoms occupy essentially equivalent positions in the lattice. Minor variations in the relative number of these atoms do not significantly alter the crystal structure or physical properties of the zeolite. For zeolite A, the "x" value normally falls within the range 1.85±0.5.
The value for "y" is not necessarily an invariant for all samples of zeolites. This is true because various exchangeable ions are of different size, and, since there is no major change in the crystal lattice dimensions upon ion exchange, the space available in the pores of the zeolite to accommodate water molecules varies.
The average value for "y" for zeolite A is 5.1. The formula for zeolite A may be written as follows:
1.0±0.2 Na2 O Al2 O3 1.85±0.5 SiO2.yH2 O.
In the formula, "y" may be any value up to 6.
An ideal zeolite A has the following formula:
Among the ways of identifying zeolites and distinguishing them from other zeolites and other crystalline substances, the X-ray powder diffraction pattern has been found to be a useful tool. In obtaining the X-ray powder diffraction patterns, standard techniques are employed. The radiation is the Ko doublet of copper and a Geiger counter spectrometer with a strip chart pen recorder is used. The peak heights, I, and the positions as a function of 2Θ where Θ is the Bragg angle, are read from a spectrometer chart. From these, the relative intensities, 100I/I0, where I0 is the intensity of the strongest line or peak and d the interplanar spacing in angstroms corresponding to the recorded lines are calculated.
X-ray powder diffraction data for a sodium zeolite A are given in Table I.
|X-RAY DIFFRACTION PATTERN FOR ZEOLITE A 100 I h2 + k2 + l2 d (A) I0|
1 12.29 100
2 8.71 70
3 7.11 35
4 6.15 2
5 5.51 25
6 5.03 2
8 4.36 6
9 4.107 35
10 3.895 2
11 3.714 50
13 3.417 16
14 3.293 45
16 3.078 2
17 2.987 55
18 2.904 10
20 2.754 12
21 2.688 4
22 2.626 20
24 2.515 6
25 2.464 4
26 2.414 >1
27 2.371 3
29 2.289 1
30 2.249 3
32 2.177 7
33 2.144 10
34 2.113 3
35 2.083 4
36 2.053 9
41 1.924 7
42 1.901 4
44 2.858 2
45 1.837 3
49 1.759 2
50 1.743 13
53 1.692 6
54 1.676 2
55 1.661 2
57 1.632 4
59 1.604 6
|MOST SIGNIFICANT d VALUES FOR ZEOLITE A d Value of Reflection in A|
12.2 ± 0.2
8.7 ± 0.2
7.10 ± 0.15
5.50 ± 0.10
4.10 ± 0.10
3.70 ± 0.07
3.40 ± 0.06
3.29 ± 0.05
2.98 ± 0.05
2.62 ± 0.05
The more significant values for zeolite A are given in Table B. t,141
Occasionally, additional lines not belonging to the pattern for the zeolite appear in a pattern along with the X-ray lines characteristic of that zeolite. This is an indication that one or more additional crystalline materials are mixed with the zeolite in the sample being tested. Small changes in line positions may also occur under these conditions. Such changes in no way hinder the identification of the X-ray patterns as belonging to the zeolite.
The particular X-ray technique and/or apparatus employed, the humidity, the temperature, the orientation of the powder crystals and other variables, all of which are well known and understood to those skilled in the art of X-ray crystallography or diffraction can cause some variations in the intensities and positions of the lines. These changes, even in those few instances where they become large, pose no problem to the skilled X-ray crystallographer in establishing identities. Thus, the X-ray data given herein to identify the lattice for a zeolite, are not to exclude those materials which, due to some variable mentioned or otherwise known to those skilled in the art, fail to show all of the lines, or show a few extra ones that are permissible in the cubic system of that zeolite, or show a slight shift in position of the lines, so as to give a slightly larger or smaller lattice parameter.
A simpler test described in "American Mineralogist," Vol. 28, page 545, 1943, permits a quick check of the silicon to aluminum ratio of the zeolite. According to the description of the test, zeolite minerals with a three-dimensional network that contains aluminum and silicon atoms in an atomic ratio of Al/Si=2/3=0.67, or greater, produce a gel when treated with hydrochloric acid. Zeolites having smaller aluminum to silicon ratios disintegrate in the presence of hydrochloric acid and precipitate silica. These tests were developed with natural zeolites and may vary slightly when applied to synthetic types.
U.S. Pat. No. 2,882,243 describes a process for making zeolite A comprising preparing a sodium-aluminum-silicate water mixture having an SiO2 :Al2 O3 mole ratio of from 0.5:1 to 1.5:1, and Na2 O/SiO2 mole ratio of from 0.8:1 to 3:1, and an H2 O/Na2 O mole ratio of from 35:1 to 200:1, maintaining the mixture at a temperature of from 20° C. to 175° C. until zeolite A is formed, and separating the zeolite A from the mother liquor.
As noted above, this invention involves, inter alia, a method for reducing degradation of internal egg quality. In one of its forms, this method involves a process for obtaining an egg crop having a reduced rate of internal egg quality degradation which comprises (i) preparing a poultry diet which contains from about 0.25 to about 3.0 weight percent zeolite A, (ii) feeding such diet to laying poultry, and (iii) recovering an egg crop therefrom in which the rate in decrease in Haugh unit value of the eggs is reduced as compared to the rate of such decrease under the same conditions with the same diet absent the zeolite A.
In another of its forms, the present invention relates to a method of improving the egg shell characteristics, e.g. egg shell strength of eggs from laying poultry. A convenient means of measuring egg shell strength is by measuring the specific gravity of the egg. This is simply done by immersing the egg in solutions of salt water of varying strengths. It is well known in the art that specific gravity correlates with egg shell strength. As specific gravity of the egg is raised, the strength of the egg shell is increased.
This latter method of this invention can also improve egg shell thickness, and/or decrease the number of eggs produced without shells.
The improvements of this invention are obtained by adding a zeolite to the diet of laying poultry. The zeolite can be added to regular or standard poultry feed or administered directly to the birds by some other means. The percent of zeolite (such as zeolite A) in the diet is typically from about 0.25 to about 3 or 4 weight percent, as discussed below. For this invention, chloride intake of the birds is reduced, even below the levels previously recognized as required. For leghorn-type poultry the chlorine level in the diets used in this invention are below 0.15 weight percent; more preferably, between about 0.04 and 0.10 weight percent.
The benefits of this invention can be obtained with ETHACAL® feed component, a commercially available form of sodium zeolite A.
As indicated above, Zeolite A is added to such feed formulation in small amounts by weight percent of up to about four weight percent. Greater amounts may be used, but may deprive the poultry of the desired amount of nutrients. Greater amounts are also likely to be cost ineffective. A preferred amount of zeolite A is from about one-half to about two percent by weight of the total feed formulation. A most preferred amount of zeolite A is from about 0.75 to about 1.50 weight percent of the total feed formulation.
For this invention the term "poultry" includes all domestic fowl, namely chickens, turkeys, ducks, geese, and the Corn is the principal diet for most laying poultry. A feed formulation comprising by weight percent the following is desirable:
soybean meal 10-30
zeolite A 0.25-4.0
A typical feed preparation for large scale laying hen operations comprises the following by weight percent:
|corn 62-68 Soy Bean Meal 18-24 Limestone 5-9 Alfalfa Meal 1 Phosphates 2 Sand 1-2 Vitamins, Amino Acids Salt and Other Minerals 0-1|
Calcium carbonate is usually in the form of natural limestone ground to a suitable particle size, but sometimes oyster shells which have also been suitably ground are used.
It can be appreciated that a wide variety of nutrients or foods may be included in the diets of layers, or poultry laying hens. In a controlled environment, the hens are only exposed to desired foods or food products. A typical laying ration composition contains the following:
crude protein - not less than
crude fat - not less than
crude fiber - not more than
calcium (as Ca) - not less than
calcium (as Ca) - not more than
phosphorus (P) - not less than
iodine (I) - not less than
salt (NaCl) - not less than
salt (NaCl) - not more than
(For this invention the salt content is reduced from the range given above.)
The foregoing composition may be obtained from or include the following ingredients:
Grain and processed grain by-products. Includes corn, corn hominy, corn germ meal, barley, millet, oats, rice, rice hulls, rye, sorghum, wheat and wheat shorts. These are among the energy ingredients, mostly carbohydrates with some proteins.
Plant protein products. Includes soybean oil meal, barley malt sprouts, coconut meal, corn distillers grain, corn gluten meal, cottonseed meal, pea seed, potato meal, peanut meal, rape seed meal, sunflower meal, wheat germ meal, brewers' yeast. All of these are protein sources.
Roughage or fiber. Includes dehydrated alfalfa, alfalfa hay, alfalfa leaf meal and pasture grasses. These are all fiber sources.
Animal and fish by-products. Includes blood meal, blood flour, dried buttermilk, dried whey, dried casein, fish meal, dried fish solubles, liver meal, meat meal, meat meal tankage, bone meal and dried skim milk. Anchovies, herring and menhaden are sources of fish meal.
Minerals and synthetic trace ingredients. Includes vitamins such as B-12, A, pantothenate, niacin, riboflavin, K, etc., DL methionine, choline chloride, folic acid, dicalcium phosphate, magnesium sulfate, potassium sulfate, calcium carbonate (limestone, oyster shells), salt, sodium selenite, manganous oxide, calcium iodate, copper oxide, zinc oxide and D activated animal sterol.
Molasses and animal fats are added to improve palatability and to increase or balance the energy levels.
Preservatives are also added such as, Ethoxyquin and sodium sulfite.
In general, a feed composition of this invention for laying hens should preferably contain, by weight percent, the following:
crude protein - at least about
crude fat - at least about
crude fiber - not more than about
calcium - about 2.7 to 4.1
phosphorus - at least about
iodine - at least 0.0001
sodium - about 0.1 to 0.4
chlorine - about 0.04 to 0.10
zeolite A - about 0.25 to 4.0
Eggs from relatively old commercial egg laying hens (68 weeks old) were fed diets containing 0 and 0.75% zeolite A for 9 weeks. In the last week of the study, fifty eggs from each group were placed in cold storage at 50° F. After 5 days at 50° F. the eggs were broken individually and the Haugh unit values measured. The results are shown in the following table.
|Treatment Average Haugh Units|
Basal Diet + 0% zeolite A
58.4 ± 0.7
Basal Diet + 0.75% zeolite A
59.5 ± 0.6
Statistical analysis of the data indicated that these mean values are significantly different at the 80% confidence
Another study was conducted with eggs for hatching from broiler breeder hens. In this study, 104 eggs from each group were stored at 55° F. After twenty-four hours half of the eggs from each group were broken individually and Haugh unit values were measured. The remaining eggs were stored for 8 days and then these were broken and measured. The data from this study are summarized in the following table:
|% Zeolite A Haugh Units in Feed After 1 Day After 8 Days|
0 80.0 ± 0.6
65.2 ± 0.7
1.5 82.9 ± 0.7
70.1 ± 0.8
Statistical analysis of the data after 1 day indicates that the mean values are significantly different at the 99.5% confidence level and after 8 days at the 99.99% confidence level.
The data also confirm the fact that interior quality as measured by albumin integrity degrades with time in storage.
A total of 880 commercial laying hens were divided into groups of 110 birds each and fed isocaloric diets containing various levels of chloride, Table 1.
|Diet Number and Percent Composition Dietary Ingredient 1 2 3 4 5 6 7 8|
Oyster Shell Flour
Salt (NaCl) 0.35
Zeolite A 0.00
The results of this study show that after four weeks on the diets, those birds fed diets with 0.75% zeolite A laid eggs having higher specific gravity than those laid by the birds without dietary zeolite. This difference was enhanced as the dietary chloride level was decreased, diets 6, 7 and 8, Table II.
Table III shows that only at extremely low chloride levels is egg weight affected, diets 7 and 8. This is also true for egg production effects, Table IV, diets 7 and 8.
Feed consumption after three weeks, Table V, is only significantly affected at the very lowest chloride level, diet 8. In this diet the only chloride available is that found in the corn and soybean meal components of the diet.
Table VI shows that the body weight of the birds had been significantly reduced when on the two lowest chloride diets.
In the following tables the significance of the superscripts is as follows. In the columns, data with different superscripts are significantly different, and data with the same are not, both to the 95% confidence level. For example, in Table II, the column for Two Weeks, the first entry, 1.0853±05, is significantly different from all other values in the table except the third entry; 1.0864±03. In the same column, the five values which have a "C" in the superscript are not significantly different.
The small numbers of birds used in the trials makes the statistics less definitive than desired. Although big numerical differences in some of the tables are not statistically different, they are indicative of trends.
|Specific Gravity Chloride Zeolite A Weeks Diet % % 0 2 4|
1 0.25 0 1.0881 ± 04a
1.0853 ± 05d
1.0871 ± 04c
2 0.19 0 1.0888 ± 04a
1.0868 ± 03c
1.0881 ± 04bc
3 0.25 0.75 1.0884 ± 04a
1.0864 ± 03cd
1.0888 ± 03b
4 0.19 0.75 1.0881 ± 06a
1.0871 ± 05bc
1.0882 ± 05bc
5 0.13 0.75 1.0887 ± 05a
1.0871 ± 05bc
1.0885 ± 04b
6 0.10 0.75 1.0885 ± 05a
1.0886 ± 03a
1.0907 ± 05a
7 0.07 0.75 1.0881 ± 04a
1.0881 ± 03ab
1.0904 ± 05a
8 0.04 0.75 1.0879 ± 06a
1.0872 ± 04bc
1.0907 ± 05a
|Egg Weight Chloride Zeolite A Weeks Diet % % 0 2 4|
1 0.25 0 57.6 ± 0.6a
59.8 ± 0.5ab
61.4 ± 0.5a
2 0.19 0 57.7 ± 0.5a
60.2 ± 0.5a
60.5 ± 0.8a
3 0.25 0.75 58.3 ± 0.3a
60.3 ± 0.3a
61.6 ± 0.4a
4 0.19 0.75 60.8 ± 2.8a
59.6 ± 0.5abc
61.0 ± 0.3a
5 0.13 0.75 58.1 ± 0.8a
60.3 ± 0.2a
61.4 ± 0.3a
6 0.10 0.75 57.8 ± 0.4a
58.8 ± 0.3bcd
60.7 ± 0.8a
7 0.07 0.75 58.0 ± 0.3a
58.5 ± 0.3cd
57.6 ± 0.5b
8 0.04 0.75 58.5 ± 0.5a
57.8 ± 0.6d
54.5 ± 1.7c
|Egg Production (%) Chloride Zeolite A Weeks Diet % % 1 2 3 4|
1 0.25 0 79.6 ± 3.4a
70.9 ± 2.9ab
84.3 ± 3.2ab
78.6 ± 2.5a
2 0.19 0 86.6 ± 01.3a
73.9 ± 2.1a
87.3 ± 2.0a
81.3 ± 2.2a
3 0.25 0.75 83.8 ± 2.4a
71.2 ± 2.0ab
79.3 ± 3.4ab
76.7 ± 2.9a
4 0.19 0.75 84.5 ± 1.6a
76.0 ± 1.5ab
86.6 ± 1.2ab
83.0 ± 1.2a
5 0.13 0.75 81.4 ± 2.5a
70.2 ± 2.6ab
77.9 ± 2.4b
80.8 ± 2.1a
6 0.10 0.75 75.3 ± 5.2a
69.1 ± 3.2ab
78.1 ± 3.2b
79.2 ± 2.5a
7 0.07 0.75 79.9 ± 2.3a
65.1 ± 3.1b
67.8 ± 3.8c
61.2 ± 1.4b
8 0.04 0.75 82.0 ± 1.9a
56.2 ± 2.9c
39.0 ± 3.0d
27.0 ± 2.1c
|Feed Consumption (g/bird/day) Chlor- ide Zeolite A Weeks Diet % % 1 2 3|
1 0.25 0 102 ± 3ab
103 ± 3ab
107 ± 3a
2 0.19 0 107 ± 2a
107 ± 1ab
104 ± 2a
3 0.25 0.75 102 ± 2ab
102 ± 1bc
104 ± 1a
4 0.19 0.75 107 ± 2a
109 ± 1a
104 ± 2a
5 0.13 0.75 100 ± 2b
107 ± 2ab
104 ± 2a
6 0.10 0.75 101 ± 2ab
104 ± 1ab
104 ± 1a
7 0.07 0.75 99 ± 2b
98 ± 2c
102 ± 2a
8 0.04 0.75 97 ± 2b
90 ± 3d
94 ± 3b
|Body Weight (g) Chloride Zeolite A Weeks Diet % % 1 2 3 4|
1 0.25 0 1597 ± 21a
1643 ± 23a
1654 ± 21a
1673 ± 20a
2 0.19 0 1593 ± 18c
1631 ± 19ab
1646 ± 23a
1646 ± 18a
3 0.25 0.75 1594 ± 25a
1605 ± 22ab
1611 ± 21ab
1636 ± 22a
4 0.19 0.75 1599 ± 23a
1640 ± 24ab
1655 ± 26a
1662 ± 23a
5 0.13 0.75 1558 ± 16a
1623 ± 21ab
1628 ± 11a
1649 ± 12a
6 0.10 0.75 1545 ± 21a
1576 ± 17bc
1594 ± 16ab
1625 ± 15a
7 0.07 0.75 1571 ± 12a
1542 ± 22cd
1556 ± 19bc
1564 ± 18ba
8 0.04 0.75 1568 ± 20a
1513 ± 22d
1494 ± 38c
1548 ± 20b
A total of 42 end of lay (60 week old) broiler breeders were housed in individual cages and divided into three groups of 14 birds each. The groups were fed the following diets:
|Diet Ingredient, % 1 2 3|
Corn 75.81 75.81 75.81
Soybean Meal 15.00 15.00 15.00
Oyster Shell Flour
6.24 6.24 6.24
0.33 0.33 0.33
D,L-Methionine 0.08 0.08 0.08
0.25 0.25 0.25
Salt (NaCl) 0.48 0.00 0.00
Sand 1.81 0.00 0.79
HCl 0.00 0.79 0.00
Zeolite A 0.00 1.50 1.50
Chloride Content, %
0.328 0.325 0.035
The results of this study are listed in the following tables:
|Broiler Breeder Egg Production (% Hen Days) Chloride Zeolite A Weeks Diet % % 1 2 3 4 5 6 7 8 9 10|
0.32 0 60.2a
0.325 1.5 54.1a
0.035 1.5 62.2a
|Specific Gravity Chloride Zeolite A Weeks Diet % % 1 3 5 7 9|
|Shell Thickness Chloride Zeolite A Weeks Diet % % 1 3 5 7 9|
1 0.328 0 .035a
2 0.325 1.5 .036a
3 0.035 1.5 .036a
|Shell Thickness Chloride Zeolite A Weeks Diet % % 1 3 5 7 9|
1 0.328 0 8.74a
2 0.325 1.5 8.86a
3 0.035 1.5 9.03a
|Shell-less and Membrane Egg Production (%) Chloride Zeolite A Weeks Diet % % 1 2 3 4 5 6 7 8 9 10|
|Broiler Breeder Collectable Egg Production (% Hen Days) Chloride Zeolite A Weeks Diet % % 1 2 3 4 5 6 7 8 9 10|
|Broiler Breeder Body Weight (g) Chloride Zeolite A Diet % % Week 1 Week 9 GAIN|
1 0.328 0 3444a
2 0.325 1.5 3264a
3 0.035 1.5 3197a
|Broiler Breeder Feed Consumption Chloride Zeolite A Weeks Diet % % 1 2 3 4 5 6 7 8 9 10|
Examples 1 and 2 demonstrate quite clearly that use of zeolite A in poultry feed improves the resistance of the eggs to internal quality degradation over time. Besides enhancing egg quality during storage and transportation, these results are of importance in that the longer hatching eggs are stored prior to incubation, the lower will be the percentage of hatch. Thus, the invention provides eggs with superior characteristics both as regards "freshness" for human consumption, and enhanced hatchability. Indeed, some published studies indicate that chicks hatched from eggs stored for over 8-10 days perform more poorly when fed to market weights. Thus, improvements in these characteristics should likewise be achieved by utilization of this invention.
In Example 4 hens fed the zeolite A, low chloride diet laid eggs with the highest specific gravity, shell thickness and percent shell, and they produced the lowest percentage of shell-less or membrane eggs, Tables IX, X, XI, and XII. Hens fed the zeolite A diets containing high chloride, equivalent to the chloride level of the control diet (Diet 1), also laid eggs with the same improvements, but of lesser magnitude than the low chloride fed hens. These improvements result in the production of more collectable eggs (Table XIII), which with broiler breeder hens means more eggs that are settable in incubators. Tables VIII and XV show that inclusion of zeolite A in the diets have no adverse effect on overall egg production or feed consumption. Table XIV shows that inclusion of zeolite in the diet results in increased weight gain suggesting that improved feed utilization is occurring.
The above results suggest the use of zeolite A levels in the diet of from about 0.25 to about 3.0 weight percent, or up to about 4.0 weight percent. The above results also suggest that for chickens, both layers and broiler breeders, the chlorine content (% chloride) in the diet can be less than about 0.15 weight percent. Preferably the chloride content is from about 0.04 to about 0.10 weight percent.
The results set forth in the Examples above demonstrate the advantages of this invention in improving interior egg quality, egg shell quality, shell thickness, percent shell, or in reducing the number of shell-less or membrane eggs. Any such benefit other than improved interior egg quality, for the purposes of this invention is referred to as an improved egg shell characteristic.
The above results demonstrate an improved process for enhancing either or both of (i) interior egg quality and (ii) an egg shell characteristic which comprises regularly feeding to laying poultry a diet which comprises a zeolite such as zeolite A. To achieve the benefits of (ii) the diet also contains a reduced chlorine content. As shown, the diets with reduced chlorine content can contain an amount of chlorine (as chloride) which is below that which was previously recognized as required. Such diets are referred to herein as chloride deficient diets. These diets can comprise the chloride content supplied by the corn and soybean meal or similar portions of the diet without any added chloride source (such as salt, i.e. NaCl), or with an amount of salt or other chloride below that which is commonly added to the diet, as illustrated above.
Generally speaking, one uses enough zeolite such as zeolite A to provide the benefit or benefits conferred by such additive. Preferred ranges are set forth above. Besides processes comprising feeding birds, this invention also provides chloride deficient diets or feed formulations which have zeolite and chloride contents as discussed above. Preferred aspects of this invention comprise use of zeolite A diets (including chloride deficient diets of zeolite A) for poultry, although diets of this invention can be used in other aspects of animal husbandry such as in the care and feeding of endangered, wild species. Of the poultry species, chickens, including layers and broiler breeders are highly preferred. Use of the diets of this invention provides improved methods of poultry farming which can result in more eggs that are usable, and which have desirable characteristics.
Having described this invention and its benefits in detail above, it will be apparent that those skilled in the art can make modifications and changes of the invention as above-described without departing from the spirit and scope of the claims which follow.