Alas, poor "magic"! I knew thee well.
mkennedy at chmeds.ac.nz
Sun Mar 13 05:03:28 EST 1994
In article <9403110150.AA17071 at pines2.ljcrf.edu>, helgew at LJCRF.EDU writes:
> Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz) wrote:
> [lotsa stuff deleted]
>>1. Grow 1.5ml O/N in 2xYT.
> [more deleted]
> does this work with LB as well?
I used to use LB, but the yields never seemed as good; 2xYT is richer, so that
makes sense. A lot of folk also seem to use terrific broth (TB), but the
yields from 2xYT cultures seem good enough for most purposes.
One point worth mentioning is that I still do 35-S manual sequencing; one of
the people in our Lab just tried getting some sequencing of these types of
preps done with 2 different ABI fluorescent machines, with no luck. It seems
additional clean up steps are necessary to get this to work.
NNNN NN Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz) ZZZZZZZ
NN NN NN Cytogenetic and Molecular Oncology Unit ZZZ
NN NN NN Christchurch School of Medicine ZZZ
NN NNNN Christchurch, New Zealand ZZZZZZZ
Phone (64-3)364-0880 Fax (64-3)364-0750
More information about the Methods