Organic Medicine for the Planet
Information about the Medical Marijuana Cannabis Testing Kit
It started back around 2005 the kit was developed to measure the primary substances in marijuana to determine the medical benefits. It was found through research that certain elements in the plant were more medically beneficial then others.
** This kit is a “Thin Layer Chromatography” testing kit designed specifically for testing Medical Marijuana sometimes called HPTLC. It is a patented and registered product, and was certified by the University of Leiden.
All though THC was the most recognized for its euphoria, relaxing type feeling, other substances such as CBD were more recognized for their pain relief and so on. The kit has been used for years in the Netherlands in coffee houses and dispensaries but never took off for individual users in Europe, especially since medical marijuana is not an issue. The manufacture went into low gear and the kit was never entered into the US market.
Due to the changing times, laws and attitudes over the years, we now have come to a time were medical marijuana is now becoming legal and the need for testing is going to become more needed if not mandated by the government.
This kit will provide you with visual color indicators for the THC, THCV, CBG, CBNV, CBD substances and more in Medical Marijuana, There is testing for estimated THC % with visual indicators, and testing for acids and poisons
This kit is a good step in providing a balanced product that provides a mild mind and body relief for medical marijuana patients and a way for growers to analyze there products during production to produce these products, and an alternative to laboratory testing.
The manufacture is constantly testing and working toward improving the kits, so we can test the many substances in Medical Marijuana, to uncover the complete potential of the plant for medical purposes.
Here is some information that will come with the kit:
You get step by step directions with pictures of each step being taken
You get all the little reaction chambers and liquids, developing jar, capillary tubes, and more, needed to perform the entire testing.
Now included with every kit, Capillary tube extraction bulb, It makes it easier to take samples from reaction chambers.
You get color charts from previous certified base line tests to match with your results and a THC % chart to match estimated % of THC and other chemicals. Represented as a chart of dots – each dot represents a % point. The larger the dot the more %, you compare the dots from your results to the ones on the chart; it is a basic chromatography way of testing substances
These are some examples of the base line charts included,
I have added 2 see through Chart, (1-25 and 26-50) You can put your sample behind the chart and see the size of your sample though the dot
YOU WILL NEED: cotton balls and a good scale, latex gloves, a well ventilated working area ( a face mask would be advised) and a scanner or digital camera to copy results. ( Note: Air and Light degrade the results it is a good idea to scan the plate after finishing testing) You can save the image in any format including using photo shop, etc.
NOTE: If you really want to get into a detailed way of defining your results ( other then a visual comparision of your results to the charts ) and you know a little bit about photo editing programs, you can down load for free: http://imagej.nih.gov/ij/ ( this is a photo editing program that can produce a numberic number evaluation, based on the area or Mean of substance dot)) or try:
http://www.justquantify.com/ . In both programs you scan your results and scan the THC referance chart compare the densitiy of the dots ( shows in a numberic form) on the referance chart and compare them to the densitiy numbers or area of your sample results. that willl give you a % value based on your chart.
You must read the entire manual before starting any testing. * Check out the video one the Main Page.
Have Questions : e-mail us at: firstname.lastname@example.org- Att. Testing Kits
Here is a sample of some plates that were done making 5 lanes, The one on the left is a cold plate test, the one on the right is a hot plate test. These are examples: The CBD & CBG are brighter then can be seen here.
When testing you can do "cold testing" which shows all the acids and the other relevant substances in the product up through the THC and CBC or you can do "hot testing " which moves more of the THC up to the top by dissolving some of the acids that would be left when a cold test is done ( It is all explained in the directions. ) The bottom line is, more THC % is tested by using the "hot testing method ".You can also divide the plate up so you can do hot and cold testing on one plate.
Labs do different types of testing- Gas chromatography (GC), High-performance liquid chromatography,(HPLC )and Thin Layer Chromatography ( TLC) (* which is what our kit does - see descripitions below)*), They first have to be DEA approved to not be illegal, next the products they test have to be by registered growers, they have a variance in their results, the same plant can be tested 3 times and get 3 different results, also if you took the same sample to three different labs you would get three different results. Basically there is no standard procedure from lab to lab for testing Medical Marijuana because of the lack of standard testing rules by the government
We believe that Labs see themselves as the only game in town and will try and require that suppliers of medical marijuana use their high priced un-proven methods by getting government support they will position themselves as the only legal way of testing MM. Without another type of test , Suppliers will be required to pay high prices for testing in Labs which may be hard to reach and still get varied results, when they can do in- house testing at a ** Fraction of the Cost** and hassle.
3 Most common types of Lab testing
Thin Layer Chromatography (TLC) – or sometimes called( HPTLC) **(Type of Kit we Offer )
Thin layer chromatography (TLC) is a widely-used chromatography technique used to separate chemical compounds. It involves a stationary phase consisting of a thin layer of adsorbent material, usually silica gel, aluminum oxide, or cellulose immobilized onto a flat, inert carrier sheet. A liquid phase consisting of the solution to be separated dissolved in an appropriate solvent is drawn through the plate via capillary action, separating the experimental solution, whereas TLC can be configured for quantitative work, at Galbraith it is primarily used in identification tests.
High Performance Liquid Chromatography (HPLC)
High-performance liquid chromatography is a form of column chromatography used frequently in analytical chemistry. HPLC is used to separate components of a mixture by using a variety of chemical interactions between the compounds within the substance being analyzed and the chromatography column.
Gas Chromatography (GC)
A Gas Chromatograph is a chemical analysis instrument used for separating volatile and semi-volatile compounds in a complex sample. The stationary phase in GC is contained in a narrow -bored column and the mobile phase is a gas. The volatile and semi-volatile components of a sample mixture are separated based on their varying partition behavior between the mobile phase and the stationary phase.
The Reality is GC testing is not good for testing of CBD, CBN,CBG, and some of the other substances in marijuana because the machines use heat to test these substances and they all evaporate at a lower temperature then THC. So you will get some results but they will not be that accurate and The coated tubes used in the machines cost several hundred dollars apiece and are used for hundreds or thousands of tests before replacement, leading to problems from contamination and degradation of the column.
HPLC testing is complex and does do cold testing but if you are looking for the highest THC% then you must test HOT & COLD which most machines are not prepared to do. As with GC, the columns must be re-used many times, leading to contamination and degradation problems. Also calibrating the machines should be done after every batch is tested and that is not done, all these systems need expensive equipment and are costly.
Technical Comparison of the 3 labTesting Methods Click Here
*Some labs create results using TLC Retention Factor ( Rf value) Click Here for More Information
Our Method of Testing
TLC is a simple, quick, and inexpensive procedure that gives a quick answer as to how many components are in a mixture. TLC is also used to support the identity of a compound in a mixture when the Rf of a compound is compared with the Rf from the literature, In our case the cannabinoids can be identified by their specific color and specific location (Rf value) and order of appearance on the TLC plate, if carried out with the solvent system from the Kit.
A TLC plate is a sheet of glass which is coated with a thin layer of a solid adsorbent, silica. A small amount of the mixture to be analysed is spotted near the bottom of this plate. The TLC plate is then placed in a shallow pool of a solvent in a developing chamber so that only the very bottom of the plate is in the liquid. This liquid, or eluent or carrier fluid, is the mobile phase, and it slowly rises up the TLC plate by capillary action.
As the solvent moves, it will pass the little spot of cannabinoid-oil micro-droplet of 2 ul that was applied on the bottom of the plate. An equilibrium is established for each component of the mixture between the molecules of that component which are absorbed on the solid and the molecules which are in solution. In principle, the components will differ in solubility and in strength of their adsorption to the adsorbent and the other components will be carried farther up to the plate than the others. When the solvent has reached the top of the plate, the plate is removed from the developing chamber, dried (ventilation!) . After drying, the separated components (cannabinoids) now separated in the silica plates, will be visualized by spraying the plate with a dye. The dye is highly specific for cannabinoids. Every principal cannabinoid will display his own color! THC: wine red, CBD: bright yellow, CBG: orange, CBN: violet etc..
NOTE: ALSO there is UV detection of the spots under a UV lamp: the silica on the thin layer plates used in the kit contains a fluorescence, this is a component that absorbs the uv wavelength of 254 nm and which fluoresces every where except where a cannabinoïd or other organic compound is on the plate. ( This is good for finding non-organic substances in you testing ).
NOTE: If you are looking for Mold in your substances you need to Just use a microscope.
Independent Research of Our Testing Procedure Click Here
Why should you use HPTLC for botanical identification?
Other techniques can address identification only in part. They either are limited to plant parts (microscopy), have difficulties with natural variability of botanical materials (IR techniques), or focus on quantitative comparison of separated markers (HPLC).
All other techniques lack the versatility and flexibility of HPTLC for the analysis of botanicals. HPTLC is the ONLY single technique that offers a generally applicable approach to compliance with the FDA requirement of 100% botanical identification
- HPTLC is the primary chemical test for identification of all pharmacopoeia monographs on botanicals (including USP)
- The acceptance and performance of HPTLC is demonstrated by the China HPTLC Atlas, AHP monographs, and the USP Dietary Supplement Compendium
- HPTLC fingerprints do not just give a correlation coefficient but visually show the similarities and differences between samples and references on the same plate
- In addition to identity an HPTLC fingerprint can also give quantitative information (intensity of bands) about a sample for batch to batch comparison
- HPTLC can also be used for assays (potency), purity (adulteration), and stability studies (shelf life)
- HPLC testing is more time consuming and have limited detectability of non UV absorbing compounds
- Unlike HPLC, HPTLC preserves the complete sample for analysis, so nothing is "hidden".
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