Tagged Primer Design
Primers: Design thousands
of primer pairs optimized for Gateway®,
BD In-Fusion™, TOPO® and
epitope systems or add functionally
useful tags to design primers for
any expression system.
Primers: Design primers
to successfully amplify ORFs with
nested PCR. The outer primer is
designed in the UTR in a region
of no significant homology. The
inner primer pair amplifies the
PCR product generated by the outer
for Fusion Products: Designs
primer for generating N and C terminal
fusion products with functionally
Speed: Processes three
hundred 10kb sequences in approximately
Reading Frame: Automatically
maintain the reading frame of the
ORF to be amplified when adding
Codon: Automatically checks
for inframe termination codon.
Throughput Primer Search
Algorithm: Calculates primer Tm using nearest neighbor thermodynamic algorithm.
Primer Rating: Uses statistical optimization techniques
to output only the best rated primer
Search Criteria: Primers are screened for their thermodynamic
properties as well as secondary
Conditions: Optimizes all primers in a single
search run for uniform PCR cycling
Sequencing Primer Design
Primers: Sequencing primers
are designed across amplicons with
a user defined interval between
forward primers. Reverse primers
stagger on the opposing strands.
Primers for In vitro Expression Studies
Generate Precise Transcripts: Design
primers to generate sense or antisense
transcripts for in vitro expression
Data and Database Management
Projects: Multiple projects can be created.
Data of multiple experiments can
be easily managed by creating separate
project for each experiment.
Application Database: Maintains a local database for sequence
information and search results.
Web Integration: Quickly retrieves batches of ORF
sequences directly into the program
from Entrez using accession numbers
or GI numbers.
Input Formats: Supports sequences in GenBank, and
FASTA formats. GenBank and FASTA
formatted files can contain multiple
sequences for batch loading.
View Output: Primer search results can be viewed
and manipulated in any spreadsheet
program like Microsoft Excel and
Load Output: Tab delimited format of the output
file can be easily loaded into any