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probe Normal PWS AS Maternal Paternal EcoRI and HpaII digestion E E E E E E probe Page 40 20 DETECTION OF UNIPARENTAL DISOMY AND IMPRINTING BY DNA ANALYSIS Normal Sodium Bisulfide treatment PAT specific PCR OL1 OL2 UG UG UG CG CG CG OL3 MAT specific PCR Normal PWS AS Maternal Paternal PCRs with OL1 - OL2 and OL3 - OL4 PWS MAT specific PCR AS PAT specific PCR OL1 OL2 UG UG Concord forex UG UG UG UG UG OL1 OL2 PAT specific PCR OL3 CG Concord forex CG CG OL3 MAT specific PCR OL4 CG CG OL4 PAT MAT UG CG OL4 CG CG Figure 5 Schematic representation of the methylation-specific PCR.
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