Wilms' tumors, a type of cancer typically found in the kidneys of young children, might have a new weapon against them. A new therapeutic approach that one day might be used to treat some of the more aggressive types of this disease could be possible now that scientists have isolated cancer stem cells that lead to the growth of the tumors.
Wilms' tumors are the most prevalent type of tumor found in the kidneys of children. While many patients respond well if the tumors are removed early by surgery and if patients are given chemotherapy, recurrences may occur and the cancer can spread to other tissues increasing the risks to the health of the patient.
Conventional chemotherapy is toxic to all cells in the body and if given to children may lead to the development of secondary cancers when they become adults. Scientists are looking for ways to ensure that drugs are targeted specifically to tumor cells and some cells in a tumor may be more important to eradicate than others.
"In earlier studies, cancer stem cells were isolated from adult cancers of the breast, pancreas and brain but so far much less is known about stem cells in pediatric cancers," remarked Professor Benjamin Dekel, head of the Pediatric Stem Cell Research Institute and a senior physician at the Sheba Medical Center and the Sackler School of Medicine at Tel Aviv University, Israel. "Cancer stem cells contain the complete genetic machinery necessary to start, sustain and propagate tumor growth and they are often referred to as cancer-initiating cells. As such, they not only represent a useful system to study cancer development but they also serve as a way to study new drug targets and potential treatments designed to stop the growth and spread of different types of cancer.
"We have demonstrated for the first time the presence of cancer stem cells in a type of tumor that is often found in the kidneys of young children."
The researchers were able to remove parts of the tumors of cancer patients and graft them into mice. This procedure led to the growth of human tumors in mice. Cancer stem cells were identified in these tumors and it was shown that only the cancer stem cells and not the other cancer cells led to the development of new tumors upon grafting into additional mice. This process could be repeated multiple times and the animals could be used to study the development of cancer and test the action of potential new cancer drugs against Wilms' tumors.
"We identified several biomarkers, including molecules that are on the cell surface, cell signaling molecules and microRNAs, that make it possible to distinguish between cancer stem cells or cancer-initiating cells and the rest of the cells in the tumor that are less likely to lead to cancer. In further experiments, we were able to show that an antibody drug that targets one such biomarker, the neural cell adhesion molecule, was able to either almost completely or in some cases completely eradicate the tumors that we induced in mice.
"This preliminary result suggests that the cancer stem cells that we have identified, isolated and propagated may serve as a useful tool to look for new drug targets as well as new drugs for the treatment of Wilms' tumors."
Further work is needed to identify more precisely how the antibody drug used in the study (lorvotuzumabmertansine) affects cancer stem cell populations and to test the long-term suitability of the antibody drug to treat Wilms' tumors in humans.
Published in EMBO Molecular Medicine.