The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 88 kDa).
Use a concentration of 10 µg/ml.
FunctionPutative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Required for normal maturation of myeloid progenitor cells.
Immunofluorescence analysis of HeLa cells using purified ab68117 at 10 µg/ml followed by fluorescein conjugated secondary antibody.
Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% Triton X-100.
Immunoprecipitation - Anti-L3MBTL3 antibody (ab68117)This image is courtesy of an anonymous Abreview.
ab68117 used in Immunoprecipitation. Whole cell lysate from cells overexpressing L3MBTL3 tagged with GFP was loaded at 250000µg.Protein G was used in the Immunoprecipitation step. ab68117 used at 20µl/mg lysate.The western blot antibody was a rat anti-GFP polyclonal conjugated to agarose, used at a 1/5000 dilution.