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5, 1 mM EDTA, 10 glycerol), and bacteria ruptured by sonication. 26053825391. Preparation of HDAC Substrate 3H-Acetyl-lysine labeled histone protein is prepared essentially, as described by Carmen et al. IR spectroscopy has been previously employed in the study of the structure of nucleic acids, producing not only information about the individual bases, sugars, and phosphate backbone, but also providing information about the helical conformation of polynucleotides (13).
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(1983). 156. Cell Interaction and Epithelial Differentiation 55 Page 26 The active regulation of KGF and GM-CSF in fibroblasts via AP-1 is triggered by IL-1 released by keratinocytes (Fig.
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