Hofmann, Annemarie and Garfinkel, Mark D. and Meyerowitz, Elliot M. (1991) cis-acting sequences required for expression of the divergently transcribed Drosophila melanogaster Sgs-7 and Sgs-8 glue protein genes. Molecular and Cellular Biology, 11 (6). pp. 2971-2979. ISSN 0270-7306. http://resolver.caltech.edu/CaltechAUTHORS:HOFmcb91
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The Sgs-7 and Sgs-8 glue genes at 68C are divergently transcribed and are separated by 475 bp. Fusion genes with Adh or lacZ coding sequences were constructed, and the expression of these genes, with different amounts of upstream sequences present, was tested by a transient expression procedure and by germ line transformation. A cis-acting element for both genes is located asymmetrically in the intergenic region between -211 and -43 bp relative to Sgs-7. It is required for correct expression of both genes. This element can confer the stage- and tissue-specific expression pattern of glue genes on a heterologous promoter. An 86-bp portion of the element, from -133 to -48 bp relative to Sgs-7, is shown to be capable of enhancing the expression of a truncated and therefore weakly expressed Sgs-3 fusion gene. Recently described common sequence motifs of glue gene regulatory elements (T. Todo, M. Roark, K. Vijay Raghavan, C. A. Mayeda, and E.M. Meyerowitz, Mol. Cell. Biol. 10:5991-6002, 1990) are located within this 86-bp region.
|Additional Information:||Copyright © 1991 by the American Society for Microbiology. Received 26 November 1990/Accepted 7 March 1991 We thank C.A. Mayeda for assisting with the microinjection of Drosophila embryos and M.F. Yanofsky for the sequence determination of BAL 31 deletion endpoints. We also thank B.A. Hamilton and P.H. Mathers for critical reading of different drafts of the manuscript. We are grateful also to the following individuals: J.J. Bonner, V. Pirrotta, and G.M. Rubin for providing cloned DNA molecules, in some cases prior to publication; and J.W. Posakony and B.T. Wakimoto for fly strains. A.H. was supported by grant Ho1047/2-1 from the Deutsche Forschungsgemeinschaft. At the outset of this work, M.D.G. was supported by National Research Award 5 T32 GM07616, awarded by the National Institute of General Medical Sciences of the National Institutes of Health to the California Institute of Technology. The work was funded by grant GM28075 (to E.M.M.) from the National Institute of General Medical Sciences of the National Institutes of Health.|
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