Which of the following is the correct temperature at which an autoclave must be run?
Which of the following is the correct depth and pH of a Mueller Hinton plate?
-6mm and pH 7.2-7.4
-3mm and pH 6.5-6.8
-4mm and 7.2-7.4
4mm and pH 7.2-7.4
Which combination of organisms are used as QC for susceptibility testing using the Kirby Bauer method?
-S. pyogenes, P. mirabilis, N. lactamica
-P. vulgaris, S.pyogenes
-E. coli, S.aureus, P. aeruginosa
E. coli, S.aureus, P. aeruginosa
Which anaerobic bacteria is isolated on Bacteroides Bile Esculin Agar (BBE)? What color do the colonies appear as?
-B. fragilis, black colonies
-C. difficile, green colonies
-B. pertussis, yellow colonies
B. fragilis, black colonies
Kanamycin-Vancomycin Laked Blood is used to isolated which anaerobic bacteria?
Cycloserine-Cefoxitin Fructose Agar (CCFA) is used to isolate which anaerobic bacteria?
What factors is CDC Anaerobic Blood Agar enriched with?
-hemin, cysteine, and vitamin K
-X, V, and hemin
-hemoglobin, X, and V
Hemin, cysteine, and vitamin K
Bordet-gengou agar isolates which of the following?
Both Cystine-Tellurite Blood and Loeffler's medium can be used to isolate which of the following species of Clostridium?
C. diptheriae (black colonies)
What is the correct blood to broth dilution when perfoming a blood culture?
When is the best time to draw a blood culture?
- during a fever spike
- just prior to a fever spike
- just after a fever spike
just prior to a fever spike
Which gram postive cocci appears on a plate as mucoid "water drop" colonies, is sensitive to optochin and is bile soluble?
Which of the following would be the correct identifying biochemical test reaction which would differentiate Citrobacter sp. from similar organisms?
What would be the correct identifying biochemical test reaction(s) which would differentiate Enterobacter sp. from similar organisms?
VP positive, DNAase negative, Ornithine positive
Which of the following would be the correct identifying biochemical test reaction(s) which would differentiate Escherichia sp. from similar organisms?
Which of the following would be the correct identifying biochemical test reaction(s) which would differentiate Morganella sp., Proteus sp., and Providencia sp. from similar organisms?
Which of the following would be the correct identifying biochemical test reaction(s) which would differentiate Salmonella sp. from similar organisms?
You are performing a biochemical test on an isolated colony of bacteria. You perform an oxidase test, what color would a positive reaction be?
You perform an ONPG test and the reaction color is yellow. Is your result positve or negative?
If you perform a phenylalanine deaminase test and your reaction yields a green color, what is your interpretation of the reaction?
You have an organism that has an A/A, gas producing TSI reaction and is H2S positive, Phe deaminase negative, LDC (Lysine decarboxylation) negative. Which bacteria is most likely present?
You have an organism that has an A/A, gas producing TSI reaction and is H2S positive, Phe deaminase positive, and Indole positive. Which bacteria is most likely present?
You have an organism that has an A/A, gas producing TSI reaction and is H2S negative, Indole negative, LDC (Lysine decarboxylase) positive, and ODC (Oxalate decarboxylase) negative. What is the most likely organism?
You have an organism that has an A/A, gas producing TSI reaction and is H2S negative, Indole positive, and Citrate negative. What is the most likely organism?
You have a K/A, H2S producing TSI reaction with biochemical reactions of Phe deaminase negative, Indole negative, LDC (Lysine decarboxylase) positive, and ODC (Oxalate decarboxylase) negative. What is the most likely identification of the organism?
Which gram negative non fermenting bacteria are associated with cystic fibrosis patients?
Burkholderia cepacia and Pseudmonas aeruginosa
What test is used to determine sensititvity to ampiciliin when dealing with Haemophilus influenzae?
You receive a sputim sample from a patient who has recently returned from a vacation at a lake. The organism grows on BCYE (Buffered charcoal yeast agar). Which bacteria is present?
What is the cause and vector responsible for Lyme disease?
Borrelia burgdorferi/Ixodes (deer/mouse) tick
What is the proper procedure to culture and isolate Borrelia burgdorferi?
Culture in Kelly medium at 33C - examining in darkfield microscopy weekly for one month
Which obligate intracellular parasite is identified by a Giemsa stain and growth in yolk sac of chick embryo or tissue culture (McCoy cell)?
How many Mycobacteria must be present on a smear to be considered significant?
In order for a sputum specimen to be considered acceptable when processing for Mycobacteria, what type of collection must it be?
First morning, 3 consecutive mornings
If a specimen suspected of Mycobacteria contamination was received in a non sterile container, would this be criteria for rejection?
Which species of Mycobacteria are photochromogen pigment producers?
Mycobacterium marinum and mycobacterium kansasii
Which species of Mycobacterium are scotochoromogens?
Mycobacterium scrofulaceum and mycobacterium gordonae
Which species of Mycobacterium are non photochromogens?
Mycobacterium avium and mycobacterium ulcerans
If gram positive cocci are identified from a sputum sample, what are the most likely type(s) of bacteria?
Staphylococcus aureus and streptococcus pneumoniae
How does transport media differ from that of bacteria media?
Contains nutrients (fetal calf serum or albumin) and antibiotics
Describe the PCR method.
Method in which nucleic acid sequences are amplified in vitro. (Each cycle produces double the amount of desired nucleic products)