Images for Precursor B-Cell Lymphoblastic Lymphoma/Leukemia
Images are from representative mice (contributed by Rick van Etten/Scott Kogan)
Induction of murine recipients with BCR-ABL retrovirus causes 3 principal hematologic malignancies:
- MPD-like Myeloid Leukemia
- Precursor B Cell Lymphoblastic Lymphoma/Leukemia
- Histiocytic Sarcoma.
Precursor B Cell Lymphoblastic Lymphoma/Leukemia
This disease is induced by many activated forms of Abl, including v-abl and activated c-abl (Jackson and Baltimore, 1989) in addition to BCR-ABL. It is most efficiently induced as a pure disease by intraperitoneal inoculation of neonatal mice (original Abelson virus protocol (Risser et al., 1978)) or by in vitro bone marrow transduction and transplantation using marrow from non-5-fluorouracil-treated donors (Roumiantsev et al., 2001). In the latter protocol, mice do not develop the prominent leptomeningeal involvement originally described with Abelson virus but instead exhibit variable levels of frank leukemia (PB WBC usually 10-30,00/mm3), with modest involvement of peripheral lymph nodes and moderate splenomegaly (0.2-0.5 g), and usually significant involvement of the marrow (>30% blasts). Blasts are positive for B220, CD19, CD24, CD43, and BP-1/6C3, indicating an early pro/pre-B phenotype. This disease is usually oligoclonal by provirus integration and immunoglobulin heavy chain gene rearrangement status; light chain loci are usually germline. Disease is readily transplantable to syngeneic or immunodeficient secondary recipients, and the leukemic cells usually grow in vitro. Diseased mice often demonstrate hind-limb paralysis due to invasion of paraspinal regions. The cause of death in Balb/c mice is typically a catastrophic hemorrhagic pleural effusion that may arise from erosion of an innominate vein lymph node and not severe anemia.
Precursor T-Cell Lymphoblastic Lymphoma/Leukemia in the Spleen, #29772A from the study set of T. N. Frederickson
|29772A Lymphoblastic Lymphoma, Spleen 40x|
|29772A Lymphoblastic Lymphoma, Spleen 100x|
|29772A Lymphoblastic Lymphoma, Spleen 400x Example 1|
|29772A Lymphoblastic Lymphoma, Spleen, 400x Example 2|