Coumarin excitation range?
pearton at saul7.u.washington.edu
Mon Jul 17 18:15:01 EST 2000
I'd like to do a microplate assay using a peptide aminomethyl-coumarin
substrate (-AMC). I can get filters to pick up the emmission wavelength
(460nm) but the closest excitation for our (proposed) system I can find is
365nm (AMC's optimum excitation is 380nm). Does anyone have an idea of
the excitation-absorbtion profile of AMC and whether 365nm would be good
enough to give a strong enough signal? I.e. how efficient would using a
365nm source be?
ps: answers preferred via email.
pearton at u.washington.edu
"Its habit of getting up late you'll agree
That it carries too far, when I say
That it frequently breakfasts at five-o'clock tea,
And dines on the following day.
The Hunting of the Snark, Lewis Carroll
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