Development of Chemical Approaches for the Quantitative Analysis of Protein Thiol Oxidative Modifications
This project is the product of collaboration with colleagues Drs. Leslie Poole (Department of Biochemistry) and S. Bruce King (Department of Chemistry) at Wake Forest. Over the past 10 years, our groups have developed chemical probes for investigating and quantifying oxidative modifications of protein cysteine thiols focusing in particular on sulfenic -SOH species (shown here).
The probes cover a broad range of reactivity profiles to fit various applications and are compatible with analysis by mass spectrometry, Western blotting and imaging [e.g., application of the BP1 reagent in imaging of tumor samples is shown under Head and Neck Cancer Project].