Figure 3: Messenger RNA expression of Treg-associated molecules in CD4+ T cells isolated from spleen and CLN of sublingually treated mice with allergic rhinitis. Messenger RNA expression of Foxp3, IL-10, and TGF-β in CD4+ T cells of spleen (a) and CLN (b) was determined by quantitative real-time PCR analysis. The expression of each molecule was normalized to the expression of GAPD. Each data was expressed as a ratio relative to mean expression level in PBS-treated control mice. Representative results from two independent experiments containing five to seven mice in each group are shown. Significance was evaluated by an unpaired t-test. .