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Neoplasma Vol.50, p.8-12, 2003
|Title: Molecular diagnosis of Epstein-Barr virus in paraffin-embedded tissues of tumors with abundant lymphoid infiltration|
|Author: M., KORABECNA ; M., LUDVIKOVA ; A., SKALOVA ;|
|Abstract: To evaluate the significance of Epstein-Barr virus (EBV) infection in
tumorogenesis, we examined ten archival samples of acinic cell carcinomas of
salivary glands with lymphoid-rich stroma, four archival samples of
lymphoepithelioma-like carcinomas (LELC) of the urinary bladder, ten samples of
oncocytic papillary carcinoma of the thyroid (Warthin-like tumors) and one
sample of lymphoepithelioma-like carcinoma of the cervix, together 25
paraffin-embedded tumor tissues. Polymerase chain reaction (PCR) and in situ
hybridization (ISH) assays were used. The EBV genome was detected by PCR using
primers targeting the IR region. ISH was performed using EBER oligonucleotide
probes. Each examination was repeated two times. Positive PCR result was
obtained in 12% of samples only. However, this result was not confirmed by the
subsequent second PCR examination. ISH revealed negative signals in all samples.
Our results demonstrate the importance of the diagnostic strategy based on
combination at least two independent methods. PCR due to its sensitivity may
produce false positive results depending on the degree of infiltration the tumor
sample by EBV carrying lymphocytes.
|Keywords: Epstein-Barr virus, EBV associated tumors, PCR, ISH|
|Year: 2003, Volume: 50, Issue:||Page From: 8, Page To: 12|