Efficient RNA interference in zebrafish embryos using siRNA synthesized with SP6 RNA polymerase
Article first published online: 7 JUL 2005
Development, Growth & Differentiation
Volume 47, Issue 5, pages 323–331, June 2005
How to Cite
Liu, W.-Y., Wang, Y., Sun, Y.-H., Wang, Y., Wang, Y.-P., Chen, S.-P. and Zhu, Z.-Y. (2005), Efficient RNA interference in zebrafish embryos using siRNA synthesized with SP6 RNA polymerase. Development, Growth & Differentiation, 47: 323–331. doi: 10.1111/j.1440-169X.2005.00807.x
- Issue published online: 7 JUL 2005
- Article first published online: 7 JUL 2005
- Received 12 January 2005; revised 9 May 2005; accepted 24 May 2005.
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Double-stranded RNA (dsRNA) has been shown to be a useful tool for silencing genes in zebrafish (Danio rerio), while the blocking specificity of dsRNA is still of major concern for application. It was reported that siRNA (small interfering RNA) prepared by endoribonuclease digestion (esiRNA) could efficiently silence endogenous gene expression in mammalian embryos. To test whether esiRNA could work in zebrafish, we utilized Escherichia coli RNaseIII to digest dsRNA of zebrafish no tail (ntl), a mesoderm determinant in zebrafish and found that esi-ntl could lead to developmental defects, however, the effective dose was so close to the toxic dose that esi-ntl often led to non-specific developmental defects. Consequently, we utilized SP6 RNA polymerase to produce si-ntl, siRNA designed against ntl, by in vitro transcription. By injecting in vitro synthesized si-ntl into zebrafish zygotes, we obtained specific phenocopies of reported mutants of ntl. We achieved up to a 59%no tail phenotype when the injection concentration was as high as 4 µg/µL. Quantitative reverse transcription–polymerase chain reaction (RT–PCR) and whole-mount in situ hybridization analysis showed that si-ntl could largely and specifically reduce mRNA levels of the ntl gene. As a result, our data indicate that esiRNA is unable to cause specific developmental defects in zebrafish, while siRNA should be an alternative for downregulation of specific gene expression in zebrafish in cases where RNAi techniques are applied to zebrafish reverse genetics.