RECOMBINANT AND EDIBLE VACCINE : RECOMBINANT AND EDIBLE VACCINE S. Jeeva
Head, Department of Microbiology
Udaya College of Arts& Science,Vellamodi Recombinant : Recombinant Isolated the DNA segment and it is inserted into plants, animals to get new product.
Edible vaccine play a vital role in immunization.
It’s not a harmful vaccine.
Antigen or Antibody expressed can be administered orally through the editable parts of plants. PLANTS ACT AS VACCINE : PLANTS ACT AS VACCINE Cheap, easily store it harmless, easily available don’t cause side effects.
PRODUCTION OF VACCINE FROM TRANSGENIC PLANTS
DNA segment inserted to transgenic plants nuclear or chloroplast genome produce subunit vaccine.
Oral or parental administration traditional vaccine against tumour disease EXPERIMENTS: : EXPERIMENTS: Southern &Northern blot analysis: Young plant leaf Add phenol or Chloroform Separate DNA extract or genomic DNA Hybridization process A1a B1b, Crp1 & 2 IMMUNOGENCITITY HEPATITIS B : IMMUNOGENCITITY HEPATITIS B B Surface antigen expressed in potatoes
Potato accumulate HBS Ag~8.5µg/g.
Experiment shows the buffering of stomach pH can be used as global immunization Recent achievement: : Recent achievement: Plants tissues accumulate antigenic protein produce transgenic plants.
Involve production of protein specific antibody Conclusion: : Conclusion: All experiments and tests can be done with mice.
If it gives resistant capacity means can be given to patients.