Freezing cultured cells
univ0938 at herald.ox.ac.uk
Wed Dec 4 13:24:52 EST 2002
On Thu, 5 Dec 2002, Oncar wrote:
> Someone teach me to freeze cultured cells by 10%DMSO in FBS instead of
> 10% DMSO in culture medium and says cells will be in excellent
> condition, i would like to know is there anyone do so? and what's yours
> method indeed ?
we do exactly that - 10% DMSO in FCS. it works fine.
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