Antibody gene cloning
Posted 09 December 2010 - 10:54 AM
could anyone tell me how to amplify the gene sequence of an antibody having the mRNA (or cDNA)? What I mean is if there is any consensus sequence in the V-gene and C-gene despite the huge variability of these genes.
Thanks a lot in advance.
Posted 09 December 2010 - 11:59 AM
Posted 09 December 2010 - 12:17 PM
Thanks once more! Could you pass me any reference/paper? I am working with mice antibodies too.
You can use degenerated primers or a combination of primers that amplify most encountered sequences. However, these are species dependent - different for mice and humans. I know a good set of primers for mice, humans I have not done before, but I believe it is much the same.