Bimodal histogram depicting adaptive thresholds location, background intensity range and heavily stained objects range A gray level histogram that corresponds to the green channel of a typical field of view after applying a median filter (3X3). The mode of the histogram are located at m1 and m2. B and H are the adaptive thresholds. l1 is a line defined between the modes of the histogram and l2 is a line defined between the origin of the histogram and the mode at m1.
Walliander et al. Diagnostic Pathology 2013 8(Suppl 1):S37 doi:10.1186/1746-1596-8-S1-S37