Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: July 11, 2010
Publication Date: October 11, 2010
Citation: Falkenberg, S., Schmidt, T., Elsasser, T.H., Sartin, J., Buntyn, J., Carroll, J.A. 2010. Influence of two different doses of infectious bovine rhinotracheitis virus (IBRV) on immune and physiological parameters in steers [abstract]. 2010 American Society of Animal Science Meeting, July 11-15, 2010, Denver, CO. Journal of Animal Science. 88(E-Supplement 2):T21. Technical Abstract: To evaluate the effects different doses of IBRV and the impact they have on immunological and physiological parameters of cattle, 18 Holstein steers (450.11 ± 75.70 kg) were randomly assigned to either a control group or 1 of 2 IBRV challenged groups. Prior to the challenge, steers were fitted with an indwelling rectal probe, BW were recorded, and a blood sample was obtained. On d 0, steers received either an intra-nasal dose of IBRV [3 ml/nostril (Group 1) or 4ml/nostril (Group 2); Cooper strain, 1 X 10 exp 6-7 PFU] or saline (3 ml/nostril; Control). IBRV exposed steers were placed in a paddock that was isolated from the Control cattle as well as all other cattle located on the research farm. Blood samples were collected via jugular venipuncture every 24 h on d 1 and 2, and every 12 h on d 3, 4, 5, 6, and 7 post-challenge. All IBRV steers had elevated rectal temperatures (p < 0.05) as compared to Control steers by d 2, returning to baseline on approximately d 5. Serum was analyzed for interleukin-6 (IL-6), interferon-gamma (IFN), tumor necrosis factor-alpha (TNF), growth hormone (GH) and insulin-like growth factor-1 (IGF-1). A difference (p <0.05) was observed in IFN, there was an increase in IFN for the IBRV challenged steers as compared to the Control cattle. No differences (p > 0.05) were observed for the other parameters of interest; TNF, IL-6, GH and IGF-1 between Control and IBRV challenged steers. We observed an increase in TNF and IL-6, however there were no differences (p > 0.05) in these concentrations as compared to the Control steers. There was a difference between the Control and IBRV steers for IFN, but no differences were observed between the 2 IBRV groups for parameters of interest. The results indicate that both doses elicited an immune response, however there was no differences in the 2 doses. It is plausible that the minimal differences observed in the parameters of interest could be a result of the sampling intervals.