James E Keller
Affiliation: Food and Drug Administration
- Uptake of botulinum neurotoxin into cultured neuronsJames E Keller
Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
Biochemistry 43:526-32. 2004..This study demonstrates that uptake of both BoNT-A and -E is enhanced with synaptic activity and that translocation of the toxin catalytic moiety into the cytosol occurs at different rates for these two serotypes...
- Comparison of extracellular and intracellular potency of botulinum neurotoxinsFang Cai
Laboratory of Bacterial Toxins, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
Infect Immun 74:5617-24. 2006..We conclude that EDTA directly alters both BoNTs in a manner that is independent of Zn2+...
- Recovery from botulinum neurotoxin poisoning in vivoJ E Keller
Laboratory of Bacterial Toxins, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, 29 Lincoln Drive, HFM 434, Room 122, Bethesda, MD 20892, USA
Neuroscience 139:629-37. 2006..Combinations of botulinum neurotoxin A and B and botulinum neurotoxin A and E were tested and shown to exacerbate paralysis compared with individually administered serotypes...
- Characterization of new formalin-detoxified botulinum neurotoxin toxoidsJames E Keller
Division of Bacterial, Parasitic and Allergenic Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
Clin Vaccine Immunol 15:1374-9. 2008..The superior quality of these new toxoids makes them useful tools for continued use in ELISA development and for antitoxin production...
- Development of a delivery vehicle for intracellular transport of botulinum neurotoxin antagonistsMichael C Goodnough
Department of Food Microbiology, Food Research Institute, University of Wisconsin, 1925 Willow Dr, Madison, WI 53706, USA
FEBS Lett 513:163-8. 2002..The HC of BoNT/A thus offers promise as a selective carrier to deliver BoNT antagonists to the nerve terminal cytoplasm for inhibiting the proteolytic activity of internalized BoNT/A LC...
- A capillary electrophoresis technique for evaluating botulinum neurotoxin B light chain activityMichael Adler
Neurotoxicology Branch, Pharmacology Division, U S Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, Maryland 21010, USA
J Protein Chem 22:441-8. 2003..The fragments and parent peptide were clearly resolved by CE, allowing accurate quantification of the BoNT/B LC-mediated reaction rates. The results indicate that CE is suitable for assessing the enzymatic activity of BoNT/B LC...