Cloning large fragments
kramer at mcbsgi.bio.sunysb.edu
Thu Sep 2 10:35:07 EST 1993
I need to make a multistep construction that approaches
30 kb. I was wondering what hints, help, and warnings
y'all might have concerning such an undertaking. Some
of the questions I have are as follows:
Are pUC based vectors somewhat limited in their
ability to carry large segments...Is pBR better
or is there something else.
Are certain bacterial strains more or less disposed
for the task?
Are there any tricks and rules-of-thumb concerning
ligations involving large inserts (20kb?)
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