25th May 13:10
polycystic ovary syndrome
in polycystic ovary syndrome, excessive oxidative stress and depletion of
antioxidants may contribute to ovarian mesenchymal hyperplasia.
Hum Reprod. 2004 Jun 3 [Epub ahead of print] Related Articles, Links
Proliferation of ovarian theca-interstitial cells is modulated by antioxidants
and oxidative stress.
Duleba AJ, Foyouzi N, Karac M, Pehlivan T, Kwintkiewicz J, Behrman HR.
Department of Obstetrics and Gynecology, Yale University School of Medicine,
333 Cedar Street, New Haven, CT 06510, USA.
BACKGROUND: Maintenance of ovarian homeostasis requires precise regulation of
proliferation of thecal- interstitial (T-I) cells. Recent evidence indicates
that oxidative stress and antioxidants modulate proliferation of various
tissues under both physiological and pathological conditions. This study
evaluated the effects of oxidative stress and antioxidants on T-I
proliferation. METHODS: Rat T-I cells were cultured in serum-free medium and
proliferation was assessed by determination of DNA synthesis using the
thymidine incorporation assay, by
3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and by
direct counting of steroidogenically active cells and steroidogenically
inactive cells. RESULTS: Antioxidants and reactive oxygen scavengers induced a
dose-dependent decrease of T-I proliferation. Vitamin E succinate was
inhibitory at 10-100 micro mol/l, ebselen was inhibitory at 0.3-30 micro mol/l,
and superoxide dismutase was inhibitory at 300-1000 IU/ml. In contrast,
oxidative stress resulted in a biphasic effect. Modest oxidative stress induced
by 1 mmol/l hypoxanthine and xanthine oxidase (3-30 micro U/ml) stimulated
proliferation of T-I cells, while greater oxidative stress induced by xanthine
oxidase (1 mU/ml) profoundly inhibited proliferation. Direct cell counting
demonstrated comparable effects on steroidogenically active and inactive cells.
CONCLUSIONS: Reactive oxygen species may play a role in the regulation of
growth of ovarian mesenchyme. Under pathological conditions, such as those
encountered in polycystic ovary syndrome, excessive oxidative stress and
depletion of antioxidants may contribute to ovarian mesenchymal hyperplasia.
PMID: 15178657 [PubMed - as supplied by publisher]
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