i am about to do ELISA for my Phd projects. In the assay i have to coat the plates with E.coli. i have read that some researchers fixed their bacterial cell with 1% formaldehyde prior to coating them onto the plate and dry them out overnight in 37C. can somebody explain to me why do the need to fix the cell first? wouldn't it will effect the result as compare to non fix-cell? and what are the effects of drying them out?
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fixing bacterial cells in ELISA
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