kDa limit coli expression
soenke.behrends from utoronto.ca
(by soenke.behrends from utoronto.ca)
Mon Sep 10 06:30:43 EST 2007
does anyone know of a size limit for expression of proteins in E.
coli? I have not found anything definite neither in the literature nor
on the internet. Have codon optimized artificial genes pushed the
limits? What are the largest proteins that have been successfully
expressed as soluble proteins. Is it reasonable to assume that protein
expression in E. coli is difficult above 50 kDa and impossible above
Thanks for your kind help!
More information about the Methods