Chromatin immunoprecipitation (ChIP) experiments often yield very small amounts of DNA. Unfortunately, most next-generation sequencing (NGS) library preparation methods use ligation to add sequencing adapters and require a larger amount of DNA. The DNA SMART ChIP-Seq Kit eliminates this problem; instead of using ligation, Illumina® sequencing adapters are added directly to low-input ChIP DNA samples via a novel, SMART-based template switching technology. Eliminating ligation from library preparation means only a single, combined size selection and clean-up step is needed, leading to higher yield and a simpler workflow.
- Flexible DNA inputs: 100 pg–10 ng of either ssDNA or dsDNA, or total DNA from 10,000–1,000,000 cell ChIP experiments
- Illumina-ready sequencing libraries in around 4 hours: Illumina indexes and adaptors incorporated into the workflow
- Excellent sequencing metrics: High non-redundant rate, high reproducibility between different sample inputs, and high overlap with data generated by the ENCODE project
Learn about all of our products for NGS: www.clontech.com/NGS