Figure 3: Fold repression caused by (a) siRLuc-I and (b) siRLuc-II on the reporter vectors pRluc and pFLuc-r. For transfection, the H1299 cells were grown in 6 cm Petri dishes at 70% confluence. One microgram (1 μg) pSEAP2-Control, 1 μg pRLuc or pFLuc-r, and siNC or siRLuc (20 nM final concentration) were mixed and transfected into the cells. Measurement of fold repression is mentioned in Section 2. The data presented are the mean ± SD of triplicates. values are also indicated.