Best way to size select cDNA ?
Thomas H. Rude
rude0001 at acpub.duke.edu
Wed Aug 10 13:47:56 EST 1994
>In article <DSMITH-100894102504 at bio-mac9.utu.fi>,
>Dave Smith <DSMITH at UTU.FI> wrote:
>>I'm in the process of making a couple of cDNA libraries. Does anybody have
>>a good method for size selecting cDNA ? I've so far been using a
>>Stratagene Unidirectional cDNA synthesis kit (works well) and then using a
>>Pharmacia S-400 spun column to isolate fragments greater than ~400 bp. I
>>would prefer to isolate cDNA larger than 1.5 kb. I've tried cutting out
>>fragments from an agarose gel and extracting with Quiaex but had a poor
>>recovery of cDNA. Any ideas? what method do you use ?
Try using BRL's (Gibco-BRL) cDNA Size Fractionation Columns.
They have worked well for us.
Duke Medical Center
E-mail Rude0001 at mc.duke.edu
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