dbell at fresno.ars.usda.gov
Tue May 11 13:23:03 EST 2004
There are a lot of things that might be happening.
If you are using a capillary electrophoresis, There are always a lot of
peaks that run through the capillary very early - these are excess
primers. My gut instinct says that there is something happening with
Are you sure this is 75 "BASE PAIR"? On my machine, the raw data from a
run comes out on a time scale - so the x axis does not get converted
into "base pairs" until you assign a start point for the analysis.
And the software will sometimes switch the SCALE of your graph (both
the x and y axes). When viewing 2 different samples, make sure you are
comparing them at the same scale.
Hope this brings some insight. If I knew more about your set-up I may
be able to help some more.
From: owner-methods at hgmp.mrc.ac.uk [mailto:owner-methods at hgmp.mrc.ac.uk]
On Behalf Of emad
Sent: Friday, May 07, 2004 1:34 AM
To: methods at hgmp.mrc.ac.uk
While I am running AFLP with fungal DNA I used water as control in one
run in the selective amplification step then I proceed to the step of
loading in the automated sequencer.
What made me astonished is that the H2O controls produce a pattern of
bands till 75 bp.
Does any one know an explanation for what happened here?
best regards .
nagref - greece
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