- F. Xu and H. M. Joon and K. Trabbic-carlson and A. Chilkoti and W. Knoll, Surface plasmon optical study of the interfacial phase transition of elastinlike polypeptide grafted on gold,
Biointerphases, vol. 3 no. 3
pp. 66 -- 74 .
(last updated on 2009/09/02)
The conformational changes in elastinlike polypeptides (ELPs) grafted to a solid/solution interface via different architectures were studied using surface plasmon resonance spectroscopy and surface plasmon field-enhanced fluorescence spectroscopy (SPFS). SPFS provides a simple and convenient optical method to study the influence of the grafting method and the graft density on the conformational changes in ELPs at the solid-solution interface as a function of environmental variables. A typical response of the ELP, consistent with its stimuli responsiveness, was a gradual collapse upon increasing the ionic strength; this effect was inversely correlated with the surface graft density of the ELP. (C) 2008 American Vacuum Society. [DOI: 10.1116/1.2965133]