qRT-PCR analysis of clustered miRNAs in scrapie-infected N2a cells. Ten ng of total RNA from ScN2a and N2a-mock cells were applied to miRNA-specific cDNA synthesis. Subsequent qRT-PCR was performed using equivalent amounts of 1.3 ng RNA. Relative miRNA expression was analyzed by ΔΔCT method  using the non-regulated miRNA mmu-miR-106b* as a housekeeping RNA and the non-infected cells as a calibrator. Statistical significance was determined by student-t-test (non-parametric with Welch’s correction, *** p < 0.001). The mean regulation factor ± SD of duplicates from three independent experiments are shown. Abbreviation n.d.; not detected.
Montag et al. BMC Genomics 2012 13:486 doi:10.1186/1471-2164-13-486