Transgenic Mice Expressing Porcine Prion Protein Resistant to Classical Scrapie but Susceptible to Sheep Bovine Spongiform Encephalopathy and Atypical Scrapie
Figure 2. Brain atypical proteinase K–resistant prion protein (PrPres) of porcine PrP transgenic mice infected with cattle bovine spongiform encephalopathy (BSE) (lane 2) or sheep BSE agents (lane 4). Electrophoretic profiles and antibody labeling of PrPres detected with monoclonal antibodies Sha31 (A) or 12B2 (B). Profiles produced by cattle (lane 1) and sheep BSE (lane 3) before passage in the porcine mouse model are shown for comparison. MW, molecular mass in kilodaltons. C) Triangular plot of the glycosyl fractions of PrPres after proteinase K digestion and Western blotting using the Sha31 antibody. Data shown are the means of 5 or more measurements obtained from density scans in 2 or more Western blots. To interpret the plot, read the values for the diglycosyl, monoglycosyl, and aglycosyl fractions along the bottom, right and left axes of the triangle, respectively. For each point, the sum of the 3 values is 100.