Quantification of the expression of ALP, BSP, OCN, OSX and Runx2 by real-time PCR. Real-time PCR was used to measure the mRNA levels of ALP, BSP, OCN, OSX and Runx2 mRNA in Saos-2 cells incubated with OM. The expression was normalized to the expression of GAPDH. The results indicate that OM can stimulate transcription of these osteoblast-related genes in Saos-2 cells than in than cells without stimulation. The also indicates that AZ can significantly decrease the expressions of these osteoblast-related genes except OSX in Saos-2 cells compared to that in OM alone. This experiment was repeated three times and similar results were obtained. * = P < 0.05, ** = P < 0.01, *** = P < 0.001. OM, osteogenic medium.
Chang et al. Arthritis Research & Therapy 2012 14:R176 doi:10.1186/ar3929