Schwann cells need to be passaged to maintain their size. Cells were cultured in serum-containing medium, with or without passaging on day 4. In both cases, 100,000 cells were plated per well, and the medium was changed every day. Mode cell volume (a) and cell number (b) were measured every day in a Coulter Counter. The experiment was performed twice with similar results.
Conlon and Raff Journal of Biology 2003 2:7 doi:10.1186/1475-4924-2-7