dmicklem at cmgm.nospam.edu
Thu Jun 24 15:57:07 EST 1999
In article <3772755D.2BC2DBBE at purdue.edu>, Phillip San Miguel
<pmiguel at purdue.edu> wrote:
Given that oxacillin and nafcillin both appear to still be useful against
multi-drug-resistant staphylococcus infections (including
vancomycin-resistant strains), do you think its really a good idea to
encourage their widespread use in laboratories? Presumably there could be a
risk of accidentally selecting in the lab for resistant staph strains.
Use of carbenicillin, or plating a larger number of plates at lower density
are usually sufficient to prevent the growth of satellite colonies... I
didn't have a problem getting lawns using the gamma-delta transposon system
as described by Strathman, PNAS 88, 1247,1991, although he does (IIRC)
mention that it can be a problem if the cells are plated at too high
(both from an Altavista search with oxacillin)).
I'm not a medic, so I'm quite prepared to be completely wrong about the
medical usefulness of nafcillin/oxacillin.
D.R. Micklem, Time flies like an arrow...
Beckman Institute, Fruit flies like a banana.
Stanford, Ca 94305 USA Email:dmicklem at cmgm.stanford.edu
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