Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor. plasmid DNA. The sequences reported are absolutely identical.
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=ATCC 11454 / DSM 20729 / NCDO 496 MEDLINE=89034093;PubMed=3141403; Buchman G.W., Banerjee S., Hansen J.N. "Structure, expression, and evolution of a gene encoding the precursor of nisin, a small protein antibiotic.", J. Biol. Chem. 263:16260-16266(1988).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=ATCC 11454 / DSM 20729 / NCDO 496 MEDLINE=91282469;PubMed=1905517; Steen M.T., Chung Y.J., Hansen J.N. "Characterization of the nisin gene as part of a polycistronic operon in the chromosome of Lactococcus lactis ATCC 11454.", Appl. Environ. Microbiol. 57:1181-1188(1991).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=6F3 MEDLINE=89155467;PubMed=2493449; Kaletta C., Entian K.-D. "Nisin, a peptide antibiotic: cloning and sequencing of the nisA gene and posttranslational processing of its peptide product.", J. Bacteriol. 171:1597-1601(1989).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=NIZO R5 MEDLINE=93373937;PubMed=7689965; Kuipers O.P., Beerthuyzen M.M., Siezen R.J., de Vos W.M. "Characterization of the nisin gene cluster nisABTCIPR of Lactococcus lactis. Requirement of expression of the nisA and nisI genes for development of immunity.", Eur. J. Biochem. 216:281-291(1993).