Abstract: An edible fungal polysaccharide termed as ABP was obtained by extraction with hot water, and followed successive chromatographic purification using DEAE-Sepharose Fast Flow column and Sephacryl S-300 High-Resolution column. A symmetrical peak was obtained on high-performance size-exclusion chromatography with an average molecular weight of 5.17 × 104 Da, which was named ABP, and its main components were D-glucose and D-mannose. Based on the study of methylation analysis, along with FT-IR, GC, GC-MS, 1D 1H and 13C NMR and 2D NMR (H-HCOSY, TOCSY, HMQC, and NOESY), its chemical structure was featured with a repeating unit (1→6) linking β-D-Glcp as the main backbone with (1→4)-linked α-D-Manp units. The structure of the mainly repeating units of ABP was established as: [PLEASE CHECK FORMULA IN THE PDF]
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He, J.; Zhang, A.; Ru, Q.; Dong, D.; Sun, P. Structural Characterization of a Water-Soluble Polysaccharide from the Fruiting Bodies of Agaricus bisporus. Int. J. Mol. Sci. 2014, 15, 787-797.
He J, Zhang A, Ru Q, Dong D, Sun P. Structural Characterization of a Water-Soluble Polysaccharide from the Fruiting Bodies of Agaricus bisporus. International Journal of Molecular Sciences. 2014; 15(1):787-797.
He, Jinzhe; Zhang, Anqiang; Ru, Qiaomei; Dong, Dandan; Sun, Peilong. 2014. "Structural Characterization of a Water-Soluble Polysaccharide from the Fruiting Bodies of Agaricus bisporus." Int. J. Mol. Sci. 15, no. 1: 787-797.