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Effect of surface charge on the cellular uptake of fluorescent magnetic nanoparticles
Link to Journal Abstract
We report on the nanoparticle uptake into MCF10A neoT and PC-3 cells using flow cytometry, confocal microscopy, SQUID magnetometry, and transmission electron microscopy. The aim was to evaluate the influence of the nanoparticles’ surface charge on the uptake efficiency. The surface of the superparamagnetic, silica-coated, maghemite nanoparticles was modified using amino functionalization for the positive surface charge (CNPs), and carboxyl functionalization for the negative surface charge (ANPs). The CNPs and ANPs exhibited no significant cytotoxicity in concentrations up to 500 ?g/cm3 in 24 h. The CNPs, bound to a plasma membrane, were intensely phagocytosed, while the ANPs entered cells through fluid-phase endocytosis in a lower internalization degree. The ANPs and CNPs were shown to be co-localized with a specific lysosomal marker, thus confirming their presence in lysosomes. We showed that tailoring the surface charge of the nanoparticles has a great impact on their internalization.
This study reports on the nanoparticle uptake into MCF10A neoT and PC-3 cells using flow cytometry, confocal microscopy, SQUID magnetometry, and transmission electron microscopy. The aim was to evaluate the influence of the nanoparticles' surface charge on the uptake efficiency.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Risk Exposure Group
Journal of Nanoparticle Research, 14(10): 1151 (October 2012)
Journal of Nanoparticle Research
Kralj S, Rojnik M, Romih R, Jagodic M, Kos J, Makovec D
1151 (14 pp)
Last updated on November 13, 2012
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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