The paired experimental pipeline. (a) The pipeline for generating differential genetic interactions is the same as for static genetic interactions except for a split onto treated and untreated plates in the last step. (b) Normalized colony size profiles for the same experimental replicate across the two conditions (blue) have the greatest Pearson correlation, as compared to the profiles of two experimental replicates of the same condition (green) or the profiles of different queries (red). EMAP, Epistasis MAPping.
Bean and Ideker Genome Biology 2012 13:R123 doi:10.1186/gb-2012-13-12-r123