Amino Acid Analysis is a technique that is used to quantify the amount of protein or peptide present in a sample based on its amino acid content. Samples are first digested into their constituent amino acids using 6M HCl at 110°C for 48hrs and these amino acids are then labeled with an amine-reactive quinoline derivative which allows them to be detected by UV spectroscopy. The different amino acids are then separated using High-Pressure Liquid Chromatography (HPLC) and the abundance of each amino acid is quantified by a UV detection system. Amino Acid Analysis thus allows for the absolute quantification of the amount of protein or peptide present in a given sample without labeling the protein or peptide itself.
Concentration/Quantity: 10-30μL of a 1mg/mL per sample replicate
(Or an equivalent amount of peptide or protein)
Number of Replicates: 3 replicates of a given sample is recommended
Sample Buffer: The sample buffer must not contain any amines (TRIS, Urea, Azides, etc.) or reducing agents (DTT, TCEP, BME). Please keep the buffer as simple as possible (such as H2O or PBS) and avoid other protein or peptide contaminants.