Facilities and equipment
The Transgenic/Knock-out Mouse Facility occupies approximately 1,000 square feet of dedicated, controlled access space. The core includes the following rooms:
- A 225-square-foot microinjection lab, containing a Nikon Diaphot microscope with DIC optics, a pair of Narishige micromanipulators, a Kinetic Systems air-table, a high resolution black and white video camera and monitor, a Nikon SMZ-800 stereo microscope with teaching head (for mouse surgery), a Nikon SMZ-645 stereo microscope (for embryo manipulation), a Sutter needle puller, a Narishige microforge, a Narishige beveller, a Forma CO2 incubator and a fume hood.
- A 225-square-foot tissue culture lab, containing a Coulter Z1 particle counter, a BioRad gene pulser II electroporator, a Nikon TS100 microscope, a Nuaire tissue culture hood, a Forma CO2 incubator, a tabletop centrifuge and a refrigerator/freezer.
- A 120-square-foot procedure room containing a fume hood (for ear notching and tail clipping).
- A 145-square foot animal holding room (dedicated to core mice only).
- A 150-square-foot clean cage holding room.
- A 75-square-foot office for the research associate, containing a fully networked Dell computer.
The molecular biology work associated with mouse production (DNA fragment purification, tail DNA isolation, Southern blot and PCR analysis) is carried out in Dr. Windle’s research lab. Her lab consists of approximately 900 square feet of laboratory space and is fully equipped for molecular biological studies.
In addition to the above, the core also maintains a walk-up IVIS Spectrum live animal imaging system, please contact the core manager to arrange for training and access.