How to 32P label a PCR probe?
cwen at aux.btny.purdue.edu
Fri Jan 12 17:04:21 EST 1996
bina at unixg.ubc.ca (Jim) wrote:
>Hi, Does anyone have a simple receipe for labelling a DNA probe with 32P-ATP in
>a PCR reaction?
This is my reaction:
Plasmid DNA, which contains an insert 2-5ng 1ul
10X PRC buffer 1ul
10XdNTP(dATP, dTTP and dGTP) 50uM 1ul
Primer 1, 50-100pg/ul 1ul
Primer 2 1ul
Then go to PCR.
The probe made is very hot, for more information, please check
under" in Molecular Biology" where all the hybridization were performed using PCR generated probe.
More information about the Methods