Figure 2: Photomicrographs of aortic sections stained with Verhoeff-Van Gieson. Groups are as follows: (a) control rats; rats fed, (b) fresh (FPO), (c) one-time-heated (1HPO), (d) five-time-heated (5HPO), or (e) ten-time-heated palm oil (10HPO). Thickened tunica media is observed in 5HPO and 10HPO groups [(d) and (e)], with an increased interlamellar space when compared to the control and FPO groups [(a) and (b)]. Disorganisation and fragmentation of the elastic lamellae were also observed in 5HPO and 10HPO (arrow, (d) and (e)). L: lumen; TI: tunica intima; TM: tunica media; TA: tunica adventitia. Same magnification is applied to all pictures (). Calibration bar = 50 μm.