This Core is conducting carbohydrate structure analysis of tissues, cells and
glycan-binding protein (GBP) ligands. To this end, glycans and glycoconjugates are being
isolated, fractionated and characterized by a variety of state-of-the-art analytical methods
including mass spectrometric analysis using MALDI and FAB ionization methods, and ancillary
methods such as ES-MS/MS to define the glycoforms present in cells and organs. This Core is
working to achieve high-throughput mouse and human glycomics. Information from this
Core is being deposited into the Central Database and
carbohydrate structures are being deposited into the Carbohydrate Database.
Determine carbohydrate structures elaborated by selected tissues that are thought
to have ligands for GBPs from wild-type and glycosyltransferase knockout mice provided
by the Mouse Transgenics Core (F).
Determine carbohydrate structures of selected human tissues.