Figure 3: NE and Ach on the differentiation of precursor Leydig cells into 3β-HSD-positive Leydig cells. The percentage of 3β-HSD-positive cells to total Leydig cells was determined and presented as means ± SEM. A single asterisks (*) indicates statistical significance () relative to the untreated control. CON: control group cultured with the same dilution of NE and Ach. NE: NE group cultured in the presence of 10 μM NE. Ach: Ach group cultured in the presence of 10 μM Ach. Bar = 50 μm.