Help finding a suitable scanner
u7k0201 at sunmail.lrz-muenchen.de
Mon Sep 18 10:01:33 EST 1995
I suggest you to try the following:
You will need a scanner (desktop preferred) that is able to scan transparencies or slides. A
CCD camera connected to a computer will probably work even better.
Put the stained gel on the scanner, place an appropriate ligh source above (or underneath, if
you use the CCD camera) and scan/take the image. Maybe you will have to use a colored
transparency for retaining the excitation light.
Hop that will work.
PS: Tell me, if it works!
gdavies at lshtm.ac.uk (Graham Davies) wrote:
>I am posting this for a friend who has the following problem.
>: Texas red is a fluorescent dye whose excitation wavelength is 589 nm.
>: This is bound to albumin and then put into serum. The resulting cocktail is
>: then eletrophoresed to separate the plasma proteins from the indigenous
>: albumin as well as the texas red labeled albumin. By using fluorescence I
>: would like to measure the quantity of labeled albumin present.
>: Is there a scanner that can read the whole gel and present the data to
>: a PC on which I am running NIH Image or Image Grabber.
>: If I can use a fluorescent scanner this will rapidly help to determine
>: the quantity of texas red present. One consideration to think about is that
>: albumin by itself will fluoresce but this is less than 589nm - the upper
>: fluorescent reach of the serum proteins is about 450 nm.
>: Thank you for helping
>Please reply to either gdavies at lshtm.ac.uk or i.maconochie at ic.ac.uk
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