Isolation of colonies (MCF7, MDA436 and T47D) - (Nov/11/2005 )
I've taken a flask of each of these cells, diluted them down and seeded them sparesly in a large petri dish. i now want to select colonies that have grown from a single cell, remove these and culture as normal. I've marked the dish with areas that I think have these colonies (although it's really hard to do when looking down a microscope), but how do I remove the cells now? Especially considering that this will be done in a hood without a microscope?
take the cells in the area you have marked with a pipette and a sterile tip of 200 µl...
make some trials in different dishes until you see the cells