Mycophenolic acid (MPA) does not impact on IL-21-induced signal transducer and activator of transcription 3 (STAT3) phosphorylation in antigen-naïve and CD27+ memory B cells. After pre-incubating peripheral blood mononuclear cells (PBMC) for 24 hours with or without MPA, cells were exposed to IL-21 for 15 minutes. STAT3-phosphorylation was determined by phospho-flow cytometry. Gating procedure of CD19+ B cells and CD27+IgD- memory B cells and CD27-IgD+ antigen-naïve B cells (A) and pSTAT3 of CD27+ memory (B) and CD27-IgD+ antigen-naïve B cells (C) are shown.
Eickenberg et al. Arthritis Research & Therapy 2012 14:R110 doi:10.1186/ar3835