1 more Benedicts recipe
Here's one more Benedicts soln recipe, similar to the others:
173 gms sodium citrate
100 gms sodium carbonate
17.3 grams copper sulfate
dissolve citrate & carbonate in 700 mls ddH20 (use heat if necessary). filter
solution. Dissolve copper in 100 mls dH20. Combine and add dH20 to a final
volume of 1 liter.
The "Benedict's Test" for reducing sugars is based on the ability of certain
sugars (simple or monosaccharide) to convert a blue cupric hydroxide
(Benedict's soln) to a brick red cuprous oxide precipitate. The color change
indicates the presence of a simple sugar (glucose or fructose) whereas a
negative color change indicates a complex sugar type like sucrose.
In our expts, equal amount of Benedict's soln is added to the solution to be
tested. The tubes are then put in a hot water bath for three minutes and
observed for a color change.
After our tests, the cuprix oxide solution is kept in a beaker until the
liquid evaporates. Then the dried precipitate is disposed of through chemical
Hope this enlightens some of you!
Victor Valley College
Get free e-mail and a permanent address at http://www.amexmail.com/?A=1
<< Previous Message | Next Message >>