User:Anthony Salvagno/Notebook/Research/2009/10/30/Primer Melting Conditions
I want to figure out the Tm for my primers.
I have 4 oligos to compute the melting Temp (Tm) of. Into mFold I am inputting these parameters:
- DNA sequence - there are two boxes. The left input is my oligo and the right input is the inverse compliment.
- 50mM Na+
- 2.5mM MG++
- 10uM oligo concentration
- Temp 37C