Editor: Craig Shoemaker
Nested PCR for the detection of Candidatus arthromitus in fish
Article first published online: 13 APR 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 308, Issue 1, pages 35–39, July 2010
How to Cite
Manzano, M., Giusto, C., Iacumin, L., Patthey, C., Cecchini, F., Fontanillas, R. and Comi, G. (2010), Nested PCR for the detection of Candidatus arthromitus in fish. FEMS Microbiology Letters, 308: 35–39. doi: 10.1111/j.1574-6968.2010.01984.x
- Issue published online: 1 JUN 2010
- Article first published online: 13 APR 2010
- Received 26 January 2010; revised 7 April 2010; accepted 7 April 2010.Final version published online 30 April 2010.
Vol. 310, Issue 1, 96, Article first published online: 27 JUL 2010
- nested PCR;
- Candidatus arthromitus;
- fish pathogen
Rainbow trout gastroenteritis has been related to the accumulation of segmented filamentous bacteria in the digestive tract of fish, which presents lethargy, reduced appetite and accumulation of mucoid faeces. Some authors associate the comparison of illness with the presence of viable filaments, which produce and release strings of endospores in the lumen of the gut. The segmented filamentous bacteria that could not be cultured in vitro have been related to Clostridium group I, and they have been named Candidatus arthromitus. Despite the various strategies that have been used to detect unculturable microorganisms, molecular methods have facilitated studies on culture-independent microorganisms. Direct DNA extraction from samples and subsequent study of 16S rRNA genes represent a tool for studying unculturable microbial flora. As direct detection of specific microorganisms is possible through the utilization of primers or probes annealing specific DNA sequences, the aim of this work was to design specific primers for the direct detection of C. arthromitus in fish using a nested PCR.