I'm going to do several FACS experiments using between 2 and 4 antibodies. My question may appear maive but I'm wondering if I should add each antibody to each sample separately or if I can make an antibody mix and then add the mix to each sample. Is there a risk that the antibodies bind to each other in the mix or not?
I also read a topic about not using trypsin to detach cell in culture when using Ab against membrane receptors? My targets are not receptors but are protein membrane so I guess I shouldn't use trypsin neither?
Thanks in advance for any advice
Edited by sabi67, 11 November 2009 - 09:23 AM.