- Backbone size (bp) 5800
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Chloramphenicol
Growth Strain(s)ccdB Survival
Growth instructionsccdB Survival
- Cloning method Restriction Enzyme
- 5′ cloning site StuI (unknown if destroyed)
- 3′ cloning site StuI (unknown if destroyed)
- 5′ sequencing primer CMV-F, SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
pCS2+ was digested with Stu I and ligated with the Reading Frame B Gateway cassette
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCSDest was a gift from Nathan Lawson (Addgene plasmid # 22423)
For your References section:Gateway compatible vectors for analysis of gene function in the zebrafish. Villefranc JA, Amigo J, Lawson ND. Dev Dyn. 2007 Nov . 236(11):3077-87. 10.1002/dvdy.21354 PubMed 17948311
Map generated by Addgene from full sequence supplied by depositor.