Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate). Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with NUTF2 polyclonal antibody (Cat # PAB23725).
Immunohistochemical staining of human bronchus with NUTF2 polyclonal antibody (Cat # PAB23725) shows strong cytoplasmic and membranous positivity in respiratory epithelial cells at 1:50-1:200 dilution.
Immunofluorescent staining of human cell line U-2 OS with NUTF2 polyclonal antibody (Cat # PAB23725) at 1-4 ug/mL dilution shows positivity in cytoskeleton (microtubules).
The protein encoded by this gene is a cytosolic factor that facilitates protein transport into the nucleus. It interacts with the nuclear pore complex glycoprotein p62. This encoded protein acts at a relative late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. It is thought to be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import. [provided by RefSeq