- Backbone size w/o insert (bp) 10813
Vector typeWorm Expression
Selectable markersPuromycin ; unc-119
Growth in Bacteria
SpeciesC. elegans (nematode)
- Cloning method Restriction Enzyme
- 5′ cloning site AttR4 (destroyed during cloning)
- 3′ cloning site AttR3 (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byPuromycin resistance gene from pBabePuro (Addgene). rpl-28 promoter and let-858 3'UTR sequences from pPD129.57 vector (Addgene).
Terms and Licenses
The full sequence provided is theoretical sequence based on a three way gateway reaction. The order of the elements has been confirmed by PCR, but not fully sequence verified. Expression pattern in worms is as expected.
Use this plasmid as a positive control for testing puromycin selection after single copy insertion by MosSCI at the ttTi5605 locus.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBCN27-Pmyo-2::GFP::unc-54_3'UTR was a gift from Ben Lehner (Addgene plasmid # 26347)
For your References section:Rapid selection of transgenic C. elegans using antibiotic resistance. Semple JI, Garcia-Verdugo R, Lehner B. Nat Methods. 2010 Aug 22. ():. 10.1038/nmeth.1495 PubMed 20729840
Map generated by Addgene from full sequence supplied by depositor.