Re: [Histonet] IHC background problems on chicken embryos
Your problem is related to the high levels of avidin in your
tissues. Remember avidin is sourced from "chicken eggs"!!!
In my veterinary IHC world I have pretty well given up on
using avidin-biotin based IHC on avian samples, due to the
excessive non-specific binding to various tissues,
especially liver. My recommendation is to switch your
detection system to a non-ABC based system such as Dako's
Envision or even Peroxidase-anti-peroxidase. In general the
amplification is a bit less using Envision or PAP, but the
slides are much better signal to noise wise.
Prairie Diagnostic Services
Histonet mailing list
<< Previous Message | Next Message >>