Apparatus has been developed and assembled to obtain holograms of a living cell culture by methods of digital and analog holography. The setup demonstrates the advantages of both methods and provides convenient adjustment and monitoring of its parameters for recording a series of digital holograms. Software processing of the results makes it possible to obtain information concerning the total optical thickness of the test samples and to visualize and measure changes in living neuronal cultures. Variations of the structure and manifestations of vital activity of a primary culture of brain cells have been investigated and compared.
© 2013 Optical Society of America
Original Manuscript: March 18, 2013
Published: July 5, 2013
A. I. Rybnikov, V. V. Dudenkova, M. S. Murav’eva, and Yu. N. Zakharov, "Using digital off-axis holograms to investigate changes of state of living neuronal cultures," J. Opt. Technol. 80, 457-462 (2013)