Broca (image) Neural Regeneration Research Caption To confirm if the increased nestin+ neurons in the medial septum-diagonal band of Broca came from the differentiation of immature cells, the rats were injected with BrdU after olfactory nerve transection. Using double fuorescent immunocytochemistry for nestin and BrdU, nestin+ neurons in the olfactory nerve transection group showed clear nestin immunoreactivity in cell bodies. Clear BrdU immunoreactivity was visible in nuclei labeled by red fuorescence in the medial septum-diagonal band of Broca (Figure 4B). However, nestin+/BrdU+ neurons were not observed in cells of sagittal or coronal slices. Credit Neural Regeneration Research Usage Restrictions None Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.