PINT regulates the expression of genes involved in cell proliferation and survival, including genes of the p53 pathway. (A) (Left) Significant KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways enriched in the 947 genes regulated by Pint. Center: Venn diagram representing the number of genes affected by Pint (947), and p53 knockdown in p53-restored doxorubicin-treated p53LSL/LSL mouse embryonic fibroblasts (MEFs) (B > 3). P-value represents the probability associated with the overlap between both gene sets (86 genes). (Right) Significant biologic functions of genes co-regulated by Pint and p53. The red line represents P = 0.05. (B) Predicted p53 regulatory network based on the fold change of genes affected by Pint depletion (ingenuity pathway analysis). (C) Relative Pint RNA levels after Pint or p53 depletion. Values are normalized to Gapdh and are the mean ± SD of four replicates. (D) Genes commonly affected by Pint and p53 depletion (B > 3). Colors represent transcripts above (blue) or below (red) the global median, scaled to twofold activation or repression, respectively.
Marín-Béjar et al. Genome Biology 2013 14:R104 doi:10.1186/gb-2013-14-9-r104