High background in Southern hybridization
mikko.taipale at helsinki.fi
Sun Jul 2 12:56:00 EST 2000
"David L Haviland" <David.L.Haviland at uth.tmc.edu> wrote:
> I've never played with dextran sulfate either. When asked, many years
> ago my post-doc mentor told me in no certain terms that dextran sulfate
> was a recipe for high background and not to use it.
Can you say what he meaned by high background? Is it unspecific
hybridization or just that the probe binds non-covalently all over to the
membrane? My problem is the former, unspecific hybridization signal.
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