Linearised DNA - linearised DNA (Mar/16/2005 )
I am doing some stable transfections using linearised plasmid. Each time I linearise the plasmid then purify it prior to transfection. Does anyone know if I can freeze it once linearised ?
I have frozen linear plasmids before, then successfully ligated them several weeks later after storing at -20. So i guess it works.
i do as jon and never get problems.