Figure 1: Rottlerin is cytotoxic for cells. (a) Rottlerin treatment for 24 h induced cytotoxicity, evaluated by the SRB assay, in a dose-dependent manner, with a IC50 of approximately 20 μM. (b) Cytotoxicity after 24–72 h exposure to 20 μM Rottlerin. Controls (24–72 h) were exposed to DMSO alone. (c) Cell survival expressed as % of seeded cells (control). Results are the means ± SD of at least three independent experiments in quadruplicate. ((d) and (e)) Dose- and time-dependent cytotoxicity evaluated by direct cell counting (Trypan Blue). % cytotoxicity: 100× (cell control−experimental)/cell control. compared with the control.