Caveolin-1 expression and cavins stability regulate caveolae dynamics in adipocyte lipid store fluctuation.
- Nolwenn Briand1,
- Cécilia Prado1,
- Guillaume Mabilleau2,
- Françoise Lasnier1,
- Xavier Le Lièpvre1,
- Jeffrey D. Covington3,
- Eric Ravussin3,
- Soazig Le Lay4,* and
- Isabelle Dugail1,*
- 1INSERM, U1166, Eq 6, Paris, F-75006 France
- 2Service Commun d'Imageries et d'Analyses Microscopiques, IBS, L'UNAM Université d'Angers, F-49933 Angers
- 3Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA
- 4INSERM SOPAM, U1063, Université d’Angers, IBS-IRIS Rue des Capucins, F-49100 Angers
- Corresponding Author: Isabelle Dugail, E-mail: ,
* Authorship note: Soazig Le Lay and Isabelle Dugail equally contributed as co–senior authors.
Adipocytes specialized in the storage of energy as fat are among the most caveolae-enriched cell-types. Loss of caveolae produces lipodystrophic diabetes in humans, which cannot be reversed by endothelial rescue of caveolin expression in mice, indicating major importance of adipocyte caveolae. However, how caveolae participate in fat cell functions is poorly understood. We investigated dynamic conditions of lipid store fluctuations, and demonstrate reciprocal regulation of caveolae density and fat cell lipid droplet storage. We identified caveolin-1 expression as a crucial step in adipose cell lines and in mice, to raise the density of caveolae, to increase adipocyte ability to accommodate larger lipid droplets and promote cell expansion by increased glucose utilisation. In human subjects enrolled in a trial of 8-weeks overfeeding to promote fattening, adipocyte expansion response correlated with initial caveolin-1 expression. Conversely, lipid mobilization in cultured adipocytes to induce lipid droplet shrinkage led to biphasic response of cavin-1 with ultimate loss of expression of cavin-1, -3 and EHD2 by protein degradation, coincident with caveolae disassembly. We have identified the key steps in cavin/caveolin interplay regulating adipocyte caveolae dynamics. Our data establish that caveolae participate in a unique cell response connected to lipid store fluctuation, suggesting lipid-induced mechano-tension in adipocytes.
- Received December 31, 2013.
- Accepted June 20, 2014.
- © 2014 by the American Diabetes Association.
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