Verma, Inder M. and Meuth, Nora L. and Fan, Hung and Baltimore, David (1974) Hamster leukemia virus: lack of endogenous DNA synthesis and unique structure of its DNA polymerase. Journal of Virology, 13 (5). pp. 1075-1082. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:VERjvir74
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Infectious hamster leukemia virus (HaLV) contains a DNA polymerase different from those of murine and avian viruses. No endogenous reaction directed by the 60 to 70S RNA of HaLV could be demonstrated in detergenttreated HaLV virions, nor could the purified DNA polymerase copy added viral RNA. The virion RNA could, however, act as template for added avian myeloblastosis virus DNA polymerase and the HaLV DNA polymerase could efficiently utilize homopolymers as templates. The HaLV enzyme was like other reverse transcriptases in that certain ribohomopolymers were much better templates than the homologous deoxyribohomopolymers. No ribonuclease H activity could be shown in the HaLV enzyme, but neither could activity be found in the murine leukemia virus DNA polymerase. The hamster enzyme was unique in that poly(A) ·oligo(dT) was a poor template, and globin mRNA primed with oligo(dT) was totally inactive as a template. Its uniqueness was also indicated by its subunit composition; electrophoresis of the HaLV DNA polymerase in sodium dodecyl sulfate-containing polyacrylamide gels revealed equimolar amounts of two polypeptides of molecular weight 68,000 and 53,000. The sedimentation rate of the enzyme in glycerol gradients was consistent with a structure containing one each of the two polypeptides. The enzyme thus appears to be structurally distinct from other known virion DNA polymerases. Its inability to carry out an endogenous reaction in vitro might result from an inability to utilize certain primers.
|Additional Information:||Copyright © 1974 American Society for Microbiology. Received for publication 18 January 1974. We thank Donina Smoler and Michael Paskind for help in some experiments; K. Somers and S. Kit of Baylor University for sending to us D-9 virus and performing infectivity assays: Stanley Drost for a generous supply of [3H]poly(A), and G. F. Temple for providing us globin mRNA. This work was supported by a contract fronm the Virus Cancer Program of the National Cancer Institute, Public Health Service grant no. IPOl CA-14051 from the National Cancer Institute, and grant no. VC-4E from the American Cancer Society.|
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|Deposited By:||Lindsay Cleary|
|Deposited On:||12 Sep 2006|
|Last Modified:||26 Dec 2012 09:02|
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