Optical biopsy spectroscopy was applied to diagnosis human brain cancer in vitro. The spectra of native fluorescence,
Stokes shift and excitation spectra were obtained from malignant meningioma, benign, normal meningeal tissues and
acoustic neuroma benign tissues. The wide excitation wavelength ranges were used to establish the criterion for
distinguishing brain diseases. The alteration of fluorescence spectra between normal and abnormal brain tissues were
identified by the characteristic fluorophores under the excitation with UV to visible wavelength range. It was found that
the ratios of the peak intensities and peak position in both spectra of fluorescence and Stokes shift may be used to
diagnose human brain meninges diseases. The preliminary analysis of fluorescence spectral data from cancer and normal
meningeal tissues by basic biochemical component analysis model (BBCA) and Bayes classification model based on
statistical methods revealed the changes of components, and classified the difference between cancer and normal human
brain meningeal tissues in a predictions accuracy rate is 0.93 in comparison with histopathology and
immunohistochemistry reports (gold standard).© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Yan Zhou ; Cheng-hui Liu ; Yong He ; Yang Pu ; Qingbo Li, et al.
"Brain cancer probed by native fluorescence and stokes shift spectroscopy", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 85531V (December 11, 2012); doi:10.1117/12.999463; http://dx.doi.org/10.1117/12.999463