Can anyone explain which are important for knockdown experiments to order from the following list?
3. custom or validated siRNA
4. Pre-designed siRNA set (3-4 siRNAs) with guaranteed silencing.
5. One custom or validated shRNA.
6. Pre-designed shRNA vector set (4 shRNAs) with guaranteed silencing.
7. Positive control for siRNAs
8. Negative control for siRNAs.
9. FAM-labeled negative control for siRNAs.
10. Controls for shRNAs.
11. Negative controls for mimics or inhibitors
12. FAM-labeled negative controls for mimics or inhibitors.