The Kinase-Glo® Luminescent Kianse Assay is a homogeneous method for
measuring kinase activity by quantifying a decrease in ATP levels following
kinase activity. The assay can work with virtually any kinase and substrate
combination, and it is performed in a single well of a standard multiwell plate
by adding an equal volume of Kinase-Glo® Reagent and recording
luminescence. The assay produces excellent Z´-factor values, easily
detects known kinase inhibitors, and provides accurate IC50 values.
Cell Notes 5, 5–8.
Richard Somberg, Beck Pferdehirt, and Kevin Kupcho