Help with CD data analysis.
Mon Sep 11 15:24:47 EST 1995
In article <43159f$rod at news.ox.ac.uk>, njh at mail.nerc-oxford.ac.uk wrote:
> I have just made my first tentative steps into the world of circular
dichroism and I am now
> looking for any information on interpreting those squiggly lines!! Does
anyone know of any
> article or review or basic introduction to the interpretation of CD
data? What are the
> significant wavelengths for both proteins and RNA? I am investigating an
> and looking for changes on binding in both the protein and the RNA.
> Any help appreciated. Thanks.
> njh at mail.nerc-oxford.ac.uk
Pages 190 -191 of Creighton, second ed. give a simple outline of CD.
The following references give descriptions of three different means by
which the secondary structure of proteins can be estimated from a CD
spectra (i.e. FAR UV CD spectra which are, in practice, from around 178
to 260nm; NEAR UV CD spectra however, are from around 260 to +350nm, and
are sensitive to chromophores and disulpide bonds in chiral environments
in the tertiary structure of proteins: averaging in the random coil state
of a protein, will result in no Near UV CD signal, hence these spectra are
sensitive to the tertiary structure of proteins)
Provencher et al; (1981) Biochemistry V.20 P.33-38
Johnson et al; (1987) Anal.Biochem. V.167 P.76-85
Fasman et al; (1992)PROTEINS: Struct. Funct. Gen. V.13 p.57-69
However, as a word of warning about over-interpreting CD spectra, I'd
strongly advise reading :
Vuilleumier et al; (1993) Biochemistry V.32 P.10303 -10313;
Finally, there should be lots of people in the group of Chris Dobson, in
Oxford who could tell you about CD.
Hope this helps
Laurence Tisi lct11 at uk.ac.cam.bio.mole
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