CLONING OF NITROGENASE STRUCTURAL GENES FROM THE OBLIGATE METHANOTROPH METHYLOCOCCUS-CAPSULATUS (BATH)
UNSPECIFIED. (1991) CLONING OF NITROGENASE STRUCTURAL GENES FROM THE OBLIGATE METHANOTROPH METHYLOCOCCUS-CAPSULATUS (BATH). FEMS MICROBIOLOGY LETTERS, 78 (2-3). pp. 121-126. ISSN 0378-1097Full text not available from this repository.
Southern hybridization techniques were used to examine the DNA homologies between the three nitrogenase structural genes nif H, nif D and nif K of Klebsiella pneumoniae and DNA from the obligate methane oxidizing bacterium Methylococcus capsulatus (Bath). The high degree of homology between methanotroph DNA sequences and the Klebsiella nif H and nif D genes was used to isolate and clone the corresponding Methylococcus nif H and nif D genes. Subsequent restriction analysis revealed that all three nif structural genes were contiguous in the Methylococcus genome in the order nif H, nif D and nif K, as found for the majority of other diazotrophs.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology|
|Journal or Publication Title:||FEMS MICROBIOLOGY LETTERS|
|Publisher:||ELSEVIER SCIENCE BV|
|Date:||1 March 1991|
|Number of Pages:||6|
|Page Range:||pp. 121-126|
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