Bovine Pulmonary Artery Smooth Muscle Cells, BPASMC (left). BPASMC immunolabeled for smooth muscle-specific alpha-actin (ABC method, right).
BPASMC from Cell Applications, Inc. have been used in studies relevant to pulmonary hypertension characterized by hyperproliferation of pulmonary artery smooth muscle cells, the process shown to be mediated by hypoxia-induced expression of Bcl-xl (Suzuki, 2007). Additionally, they were used to elucidate the PKC-α dependent mechanisms of proMMP-2 activation and other signaling events in smooth muscle cells under pro-inflammatory conditions related to cell growth (Chakraborti, 2009; Roy, 2011, 2013; Dey, 2012; Chowdhury, 2013; Shaikh, 2013).
Characterization: positive for smooth muscle cell specific alpha-actin expression.
Tunica intima and media of pulmonary artery.
Animal tissues were USDA inspected. Each lot is tested negative for mycoplasma, bacteria, and fungi.
2nd passage, >500,000 cells in Bovine Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO.
Ampoule of cryopreserved BPASMC (B352-05), 500 ml Bovine Smooth Muscle Cell Growth Medium (B311-500), and a Subculture Reagent Kit (090K).
Shipped in Bovine Smooth Muscle Cell Growth Medium at 3rd passage in either flasks or multiwell dishes.
Can be cultured at least 16 doublings
Chakraborti, S., A. Chowdhury, P. Kar, P. Das, S. Shaikh, S. Roy, and T. Chakraborti. 2009. Role of protein kinase C in NADPH oxidase derived O2(*-)-mediated regulation of Kv–LVOCC axis under U46619 induced increase in [Ca2+] in pulmonary smooth muscle cells. Archives of biochemistry and biophysics. 487:123-130.
Chowdhury, A., S. Roy, T. Chakraborti, K. Dey, and S. Chakraborti. 2013. Activation of proMMP-2 by U46619 occurs via involvement of p38MAPK-NFκB-MT1MMP signaling pathway in pulmonary artery smooth muscle cells. Molecular and cellular biochemistry:1-16.
Dey, K., S. Roy, B. Ghosh, and S. Chakraborti. 2012. Role of protein kinase C in phospholemman mediated regulation of α2 β1 isozyme of Na+K+ ATPase in caveolae of pulmonary artery smooth muscle cells. Biochimie. 94:991-1000.
Roy, S., T. Chakraborti, A. Chowdhury, and S. Chakraborti. 2013. Role of PKC-α in NF-κB–MT1-MMP–mediated activation of proMMP-2 by TNF-α in pulmonary artery smooth muscle cells. Journal of biochemistry. 153:289-302.
Roy, S., K. Samanta, T. Chakraborti, A. Chowdhury, and S. Chakraborti. 2011. Role of TGF-β1 and TNF-α in IL-1β mediated activation of proMMP-9 in pulmonary artery smooth muscle cells: Involvement of an aprotinin sensitive protease. Archives of biochemistry and biophysics. 513:61-69.
Shaikh, S., J. Sarkar, A. Pramanik, K. Karmakar, and S. Chakraborti. 2013. Effect of m-calpain in PKCα-mediated proliferation of pulmonary artery smooth muscle cells by low dose of ouabain. IJBB. 50:419-427.
Suzuki, Y.J., H. Nagase, C.M. Wong, S.V. Kumar, V. Jain, A.-M. Park, and R.M. Day. 2007. Regulation of Bcl-xL expression in lung vascular smooth muscle. American journal of respiratory cell and molecular biology. 36:678.