Evereh G Robert
erobert at mailhost.tcs.tulane.edu
Tue Jan 23 22:52:12 EST 1996
It is not clear to me what exactly hapens when a E.coli cell takes up a
piece of DNA during transformation. In particular, does a cell usually
take up more than one piece of DNA and do these compete for
maintainence? Or, alternatively, do the cells only take up one piece of
DNA. I am asking this question because I have been doing some difficult
clonings in E.coli which will be "shuttled" into Streptomyces lividans TK64.
I have designed my experiment such that only the correct clones will
replicate in both E. coli and Streptomyces. My uncertainty lies in
whether I need a large number of cells to transform if the desired
constructs constitute a small fraction of the total plasmid population.
I would be very thankful for any input.
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