Sporadic Oropouche Infection, Acre, Brazil
Figure. Phylogenetic tree of Oropouche virus strains; boldface shows the sample from the patient in this study. Phylogenetic tree was constructed from partial nucleocapsid gene sequence (522 nt, 27–200 aa) by neighbor-joining method implemented in MEGA 3.0 software (9). Kimura 2-parameter nucleotide substitution model was used, and the reliability of the branching patterns was tested by 1,000 bootstrap pseudo replicates. Bootstrap values (%) are shown in main nodes. Aino, Akabane, and Tinaroo viruses were used as the out group. The scale bar represents 5% nucleotide sequence divergence. GenBank accession numbers are provided and are grouped by strain designation. GI, genotype I; GII, genotype II; GIII, genotype III.