(full paper is archived in the Miller Library)
Title: Detection of sodium channel messenger RNA in the stellate ganglion, optic lobe, and mantle muscle of the squid Loligo opalescens
Student Author(s): Ozawa, Clare Risa
Faculty Advisor(s): Gilly, William
Location: Final Papers Biology 175H
Date: June 1994
Abstract: Different types of Na channels may be distinguished not only between species but also within individual organisms. Investigation in to the differences between Na channel isoforms may be useful in elucidating mechanisms by which Na channels carry out their specific functional roles within cells. In this study, two RNA probes transcribed from segments of cDNA encoding a putative squid giant axon Na channel GFLN1 were employed in insitu hybridizations and RNase protection assays to detect Na channel messenger RNAs in the stellate ganglion, optic lobe and mantle muscle of the squid Loligo opalescens. The results of this study indicate the presence of additional Na channel mRNAs with partial swquence homology to GFLN1 mRNA in squid stellate gangiona nd optic lobe, and provide evidence for the expression of Na channel mRNA in mantle muscle cells of L. opalescens.