Validation of Stable Transfectants. The motor neuron-neuroblastoma hybrid cell line, NSC-34, was transfected with empty pcDNA vector or pcDNA-hPGRN and selected for drug resistance over a three-week period. (A) Species-specific PGRN primers were used to confirm the stable integration of hPGRN. (B) Western Blot analysis confirmed presence of hPGRN in stable transfectants and not in the vector only control cells.
Ryan et al. BMC Neuroscience 2009 10:130 doi:10.1186/1471-2202-10-130