If you add growth factors to your tissue culture media (bFGF, IGF, HGF...) how long is that bottle of complete media typically still "good"? Does it vary wildly depending on the specific growth factor? I've been using recombinant human bFGF most recently. I noticed they didn't mention anything specifically in the product information or on the company web sites.
Note, I didn't mean to ask how often do you change it (feed them). I meant to ask how long is that bottle of media good with normal usage (sitting at 4deg C most of the time and then warmed up to 37 every time you use it). Do the growth factors denature, degrade or somehow otherwise go bad?
Edited by assembler01, 17 February 2012 - 12:41 PM.