EXPRSS: an Illumina based high-throughput expression-profiling method to reveal transcriptional dynamics.
BMC Genomics. 2014 May 6;15(1):341
Authors: Rallapalli G, Kemen EM, MacLean D, Robert-Seilaniantz A, Segonzac C, Etherington G, Sohn KH, Jones JD
EXpression Profiling through Randomly Sheared cDNA tag Sequencing (EXPRSS) employs adaptive focused acoustics to randomly shear cDNA and generate sequence tags at a relatively defined position (~150-200 bp) from the 3′ end of each mRNA. EXPRSS is a strand specific and restriction enzyme independent tag sequencing method that does not require cDNA length-based data transformations, reveals alternative polyadenylation, polyadenylated antisense transcripts and is highly reproducible. It is high-throughput, cost-effective using barcoded multiplexing, avoids the biases of existing SAGE and derivative methods and can reveal polyadenylation position from paired-end sequencing. Implementation of the EXPRSS method was verified through comparative analysis of expression data generated from EXPRSS, NlaIII-DGE and Affymetrix microarray and through qPCR quantification of selected genes. Unlike array-based methods, it can be applied to genomes for which high-quality reference sequences are unavailable.
PMID: 24884414 [PubMed - as supplied by publisher]