PS129α-syn accumulates in fibrillar deposits in hA30Pα-syn mice. Representative electron micrographs illustrating a cell body (A) and a process sectioned transversally (B) or longitudinally (C) and immunostained with PS129α-syn antibodies and secondary antibodies coupled to horseradish peroxidase from a representative symptomatic hA30Pα-syn mouse expressing parkin. (A’-B’) are higher magnifications of the framed regions in (A) and (B). (D) Nanogold staining of a PS129α-syn deposit within a neuronal process. Arrowheads indicate fibrils with (red) or without (black) associated PS129α-syn staining; N (nucleus), M (mitochondria). Similar results were obtained in hA30Pα-syn mice with two or no functional parkin alleles. Scale bars: 4 μm in (A) and (B), and 0.5 μm in (A’), (B’), (C) and (D).
Fournier et al. BMC Neuroscience 2013 14:135 doi:10.1186/1471-2202-14-135