Development of a method for evaluating the induction of specific immunity against tumor cells by monocytes transfection with tumor RNA.
The cancer immunotherapeutic approach has been increasingly exploited. Among the factors that make it attractive, but also limited, is the use of patient antigenic material. Thus, we propose to establish conditions for extraction and amplification of mRNA tumor, as renewable source of antigens. It is also intended to assess the vaccine effectiveness, using monocytes transfection with total tumor RNA for mimicry the tumor cells. Different concentrations (0.1 to 10 mg) of SK-BR-3 total RNA and different times (12, 24 and 48 h) were used in transfection. To assess the lympho-stimulator potential of transfected monocytes was used the test of lymphocyte proliferation, and cytokines secretion during co-culture. The result was that transfected monocytes became more activated and were able to induce lymphoproliferation. These results indicated that the development of a method for evaluating cellular responses induced against tumor cells in cancer patients who were vaccinated is possible.
Advisor:Jose Alexandre Marzagao Barbuto; Luis Eduardo Coelho Andrade; Antonio Condino Neto; Jose Alexandre Marzagao Barbuto
School:Universidade de São Paulo
Source Type:Master's Thesis
Keywords:Cancer Immunoterapy Lymphocyte proliferation Monocytes RNA Transfecção Transfection
Date of Publication:11/27/2008