nt2 / hnt2 cell question
lcalizo at mail.med.upenn.edu
Mon Dec 9 18:33:17 EST 1996
you don't HAVE to use opti-mem. the lab that developed the cell line
didn't use optimem at first either, but they found that it worked
better than the medium they were previously using. look up the nt2n
papers by virginial lee (v.m.y. lee) and see what they use.
slavemaster (un691cs at genius.embnet.dkfz-heidelberg.de)
wrote: : We are using NT-2 cells which can differntiate into
: neuron-like hNT-2 cells with retionic acid.
: I noted that the medium: opti-mem is 3-fold
: more expensive than the medium we currently use
: for (primary) cellcultures.
: Does one actually HAVE to use opt-mem ?
: Anybody out there using NT-2 cells as well ?
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