Adding IPTG and X-gal to LB plates-puzzle.
lm11 at gpu.srv.ualberta.ca
Fri Jul 11 19:31:33 EST 1997
Alex Dobrovic <adobrovic at medicine.adelaide.edu.au> wrote:
: Why is it that more IPTG and X-gal are added when one spreads them on
: previously made LB antibiotic plates than when IPTG and X-gal are included
: in the LB agar before pouring? Is it an old mistake that everyone
: replicates or is there a reason?
: Tel 61-8-8222 6884 Fax 61-8-8222 6046
Spreading Xgal and IPTG on a previously made plate is mainly to save
money, because Xgal is too expensive. Probaly you are using too much Xgal.
Try to use less.
More information about the Methods