vacoular or plasma membrane protein?
Dr Engelbert Buxbaum
(by engelbert_buxbaum from hotmail.com)
Sun Apr 3 18:01:36 EST 2011
In article <mailman.476.1300830859.20886.methods from net.bio.net>,
emad.albarouki from landw.uni-halle.de says...
> Hello everybody,
> I have a question concerning a plasma membrane protein, which have been
> expected using different proteomic software to be localized on the plasma
> After tagging with gfp it looks to be localized in more than 80% on vesicle
> structures which we assumed to be vacuoles.
> Is it possible to be a bifunctional protein such as transporter on plasma
> membrane and vacuole? or that could be an artifact of gfp fusion process?
> Is there any programs or software can discriminate between both
> compartment's proteins?
Software can make predictions, but those have to be checked by
experiment. Homogenisation of the cells and fractionisation on a sucrose
gradient in an ultracentrifuge would be a classical way of doing it, or
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