|Rodriguez, Flor - UW-MADISON|
Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: July 15, 2003
Publication Date: January 15, 2004
Citation: Rodriguez, F., Spooner, D.M. 2004. Nitrate reductase based phylogeny of wild potatoes (solanum section petota) [abstract]. Plant and Animal Genome Conference. p.80. Technical Abstract: Nitrate reductase (NIA) catalyzes NAD(P)H reduction of nitrate to nitrite, which is the first step of nitrate assimilation. The gene expressing this protein has been isolated from fungi, algae, and higher plants. NIA has three intron regions and has been shown to be single-copy in some plants, where it is useful for low-level phylogenetic analyses. NIA has been used in phylogenetic studies for Antirrhinum, Hordeum, Memecylon, Scaevola, and Tilia. We designed primers from a published (GenBank) NIA sequence of tomato (5309 bases) and 2 cDNA sequences in potato (3049 and 3066 bases). These primers flanked the three NIA introns (intron 1 74 bases, intron 2 847 bases, and intron 3 436 bases). Preliminary data from intron 3 from 35 species of diploid (2n = 2x = 24) wild potatoes (Solanum section Petota), representing the previously determined cladistic diversity of the section, and Solanum sections Etuberosum and Lycopersicon (tomatoes) as outgroups, show many points of concordance to other gene phylogenies of the group. We are exploring the phylogenetic utility of introns 1 and 2 to see the value of NOA for phylogenetic reconstruction in potato.