Thermo Scientific Pierce Hydrochloric Acid Solution is a constant-boiling 6N HCl solution, supplied in 1mL ampules, that is specifically formulated as a sequencing-grade reagent for total protein hydrolysis for amino acid analysis.
A variety of products are available for peptide hydrolysis, amino acid analysis and HPLC separation. This constant boiling HCl is specially purified to effect peptide hydrolysis at 150°C, to ensure the integrity of your amino acid analysis data and to maximize sensitivity by eliminating interferences in your amino acid analysis. Pierce Hydrochloric Acid is purified and packaged to ensure a ninhydrin negative blank on hydrolysis. Convenient, pre-scored ampule packaging of the ready-to-use HCl maintains reagent integrity. This virtually eliminates exposure to laboratory atmospheres, fingerprints and other contaminants resulting from pipetting from bulk bottles.
- Hydrolyzes peptides in 6 hours at 150°C
- Specially purified to give a ninhydrin-negative blank on hydrolysis
- Packaged in ampules to eliminate contamination and ensure product integrity
Eveleigh and Winter (1970) give an excellent description of the total protein hydrolysis technique using Constant Boiling Hydrochloric Acid. Standard protein hydrolysis conditions are 105-110°C for 16-24 hours. At 150°C, this reagent can hydrolyze peptides in 6 hours.
||Chemical structure of hydrochloric acid (HCl).
- Eveleigh, J.W. and Winter, G.D. (1970). Protein Sequence Determination, Ed Needleman, S.B., Springer-Verlag, pp 92-95.
- Blankenship, et al. (1989). High-sensitivity amino acid analysis by derivatization with o-Phthalaldehyde and 9-Fluorescence detection: applications in protein structure determination. Anal. Biochem. 178, 227-232.
- HurIey, J.B., et al. (1984). Isolation and characterization of a cDNA clone for the λ subunit of bovine retinal transducin. Proc. Natl. Acad. Sci. U.S.A. 81, 6948-6952.
- Lee, K., et al. (1979). Derivatization of cysteine and cystine for fluorescence amino acid analysis with the o-Phthaldialdehyde/ 2-mercaptoethanol reagent. J. Biol. Chem. July 25, 6248-6251.
Vacuum Hydrolysis Tubes
Amino Acid Standard H
Amino Acid Detection Reagents
Solvents for HPLC