Deep sequencing process
Products of RT-PCR amplification of MERS-CoV patient RNA (A). All expected products are 2–3 kb in length, with amplicons 9/11 and 10/11 producing 6 and 4 kb products. DNA marker sizes are shown in kb. (B) 100 000 random reads (a tenth of the entire sample dataset for Al-Hasa_19) were mapped to the Al-Hasa_1_2013 genome. The histogram shows the positions of each read across the genome with 100–1000 reads at each position across the entire genome. The actual coverage is ten times greater, yielding 1000–10 000 coverage. Open reading frame (ORF) map (C) of a successfully assembled Al-Hasa_19 genome. An open box shows open reading frames of 100 codons or greater in length. Stop codons are shown by vertical black lines. The first ATG in each ORF is shown by a vertical red line. MERS-CoV=Middle East respiratory syndrome coronavirus.