Does the postPCR 4-degree hold damage the thermal cycler ??
Jose de las Heras
josenet at tiscali.co.uk
Sun Jun 18 10:53:55 EST 2006
"DK" <dk at no.email.thankstospam.net> wrote in message
news:vmokg.1286$uz5.426 at fe04.lga...
> In article <e6t1ke$1rua$1 at agate.berkeley.edu>, lhom at OCF.Berkeley.EDU
> (Louis Hom) wrote:
>>I have no evidence that an overnight 4deg hold causes excessive wear on a
>>thermocycler, but having touched the hot side of a peltier module cooling
>>to 4deg, it wouldn't at all surprise me if it did present a problem.
> It's only a problem if the fan breaks. Peltier itself chips themselves
> can withstand high temps very well. Modern Beckman centrifuges
> have Peltier-based cooling systems - and these machines are built
> to last decades.
>>FWIW, I have done many overnight 10deg holds without ill effect on the
>>DNA. I don't know if it's any better for the instrument, but it makes me
> For long life of the instrument, occasional sacrifice of the small
> animal might be needed :-)
Shit... I am the shortest one in the lab...
Musha ring dum a doo dum a dah - www.mcnach.com
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