Majumder, Kumud and Kadalayil, Latha P and Brahmachari, SK (1987) Synthesis and Purification of Oligodeoxyribonucleotides: A Modified Approach. In: Current Science, 56 (14). pp. 693-697.
A reduction in monomer consumption in the solid phase synthesis of oligodeoxyribonucleotides has been achieved by introducing a repeat coupling approach. Additionally, a method of anion exchange HPLC- purification of the synthesized nucleotides at room temperature has been developed in which a highly volatile buffer was used as the eluent. An alternative approach of HPLC purification of oligonucleotides is also reported.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Indian Academy of Sciences.|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit|
|Date Deposited:||02 Apr 2008|
|Last Modified:||19 Sep 2010 04:44|
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