Daniel J Needleman
Affiliation: Harvard University
- Determining physical principles of subcellular organizationDan Needleman
School of Engineering and Applied Sciences, Department of Molecular and Cellular Biology, FAS Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA Electronic address
Dev Cell 29:135-8. 2014..One difficulty is that we lack an understanding of the physical principles of subcellular organization. Combining quantitative experiments with new theoretical insights may allow such principles to be developed. ..
- Cellular allometry: the spindle in development and inheritanceDaniel J Needleman
Harvard University, 52 Oxford Street, Room 365 10, Cambridge, MA 02138, USA
Curr Biol 19:R846-7. 2009..Recent studies have demonstrated a correlation between cell size and the behaviors of the cytoskeletal division machinery during embryogenesis, giving insight into how a core cellular process is modulated over the course of development...
- Fast microtubule dynamics in meiotic spindles measured by single molecule imaging: evidence that the spindle environment does not stabilize microtubulesDaniel J Needleman
School of Engineering and Applied Sciences, Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
Mol Biol Cell 21:323-33. 2010..It follows that the key to understanding spindle morphogenesis will be to elucidate how nucleation is spatially controlled...
- Mitosis: taking the measure of spindle lengthDaniel J Needleman
Harvard University, Cambridge, MA 02138, USA
Curr Biol 20:R359-60. 2010..Recent studies have investigated the mechanisms responsible for setting spindle length - and how spindle length changes over the course of development...
- Nucleation and transport organize microtubules in metaphase spindlesJan Brugués
Department of Molecular and Cellular Biology and Center for Systems Biology, Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA
Cell 149:554-64. 2012....
- Quantitative characterization of filament dynamics by single-molecule lifetime measurementsLeonid A Mirny
Harvard MIT Division of Health Sciences and Technology, and Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Methods Cell Biol 95:583-600. 2010..Similar methods should prove useful in studying cytoskeletal filaments in other contexts in vitro and in vivo...
- Plasmid segregation: is a total understanding within reach?Daniel J Needleman
Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Curr Biol 18:R212-4. 2008..Recent in vitro and in vivo studies of the proteins responsible for the active partitioning of bacterial plasmids suggest that it will be possible to develop a quantitative, molecular understanding of this form of DNA segregation...
- Pin-hole array correlation imaging: highly parallel fluorescence correlation spectroscopyDaniel J Needleman
Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA
Biophys J 96:5050-9. 2009....
- Automated identification of microtubules in cellular electron tomographyDaniyar Nurgaliev
Department of Physics, Harvard University, Cambridge, Massachusetts 01238, USA
Methods Cell Biol 97:475-95. 2010..At present, the method could be used to assist the analysis of large-scale tomography reconstructions. With further improvements, it may be possible to reliably segment cellular tomograms without human intervention...