The One-Step Rapid Nicotine Test is a simple immunoassay test that is used for the qualitative detection of Cotinine in human urine. Cotinine is the substance that is tested for when testing for nicotine use.
Confirm Biosciences’ nicotine screening test kits are based on the principle of highly specific immunochemical reactions of antigens and antibodies. They are simple and convenient tests for the rapid qualitative detection of Cotinine in human urine at a 200 ng/ml cut-off concentration.
Most experts and healthcare professionals agree that nicotine is unquestionably the most addictive drug in use today. The principle source of nicotine is tobacco products. Tobacco smoking or chewing results in the absorption of nicotine through the lung and buccal/nasal epithelium, after which nicotine is metabolized into 20 metabolites excreted in urine. Cotinine, a major metabolite of nicotine, accumulates in the body with regular smoking. It is reported that Cotinine is stable in body fluids and has a relatively long half-life of approximately 17 hours. Therefore the detection of Cotinine is less dependent on the time of sampling than that of nicotine and other metabolites.
Cotinine has been widely used as a bio-marker of tobacco exposure. Methods of analysis for Cotinine in biological fluids include gas chromatography, gas chromatography-mass spectrometry, HPLC, HPLC-mass spectrometry, EIA and RIA. These methods usually require special equipment and complicated operation procedures.
Features and Benefits:
- Easily, Reliably, & Instantly Detect Tobacco Usage
- Quick Analysis-on-site results in 5 minutes
How to Use:
Positive: One (1) pink-rose band appears in the control zone and no band appears in the test zone (for a total of one band). A positive result indicates the Cotinine level is 200 ng/ml or higher in the test urine sample.
Negative: One band appears in the control zone and one band appears in the test zone (for a total of two bands). A negative result indicates that the Cotinine level is below the detection sensitivity of 200 ng/ml.
IMPORTANT: Any line, no matter how faint, appearing in the test area confirms a negative test.
The test is invalid if there are no distinct color bands visible in both the test zone and the control zone or if there is a visible band in the test zone but not in the control zone. (Control zone band must show up.) In this instance, re-testing of the specimen is recommended.