Helen Jenkins came 14th in her final race before representing Britain at the Rio Olympic Games, with Lucy Hall 20th.
The United States' Katie Zaferes won the sprint race - her first career World Series win - ahead of Rachel Klamer and Gwen Jorgensen.
Spain's Mario Mola won the elite men's race, with Britain's Adam Bowden finishing 21st and Tom Bishop 49th.
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South Africa's Richard Murray was disqualified from second position for unsportsmanlike behaviour, after officials decided he had overreacted to a time penalty he was given at the end of the race.
Murray's disqualification meant that Jacob Birtwhistle and Fernando Alarza completed the podium.
On Sunday the team relay event takes place in Hamburg with coverage available from 13:30 BST on the Red Button. There's also highlights of the men's and women's races on Sunday at 12:30 BST.
Great Britain's Jodie Stimpson finished fourth in the elite women's race at the Triathlon World Series in Hamburg.