For demultiplexing, we employed scTagger 26, which uses a direct matching approach for barcodes. We matched Visium dataset tissue barcodes from the 10X Space Ranger output to the long reads, allowing for up to two mismatches as per scTagger's default string edit distance values. To extract the UMI region of the read, we identified the Illumina Adapter Read1 (CTACACGACGCTCTTCCGATCT) sequence as an anchor. We conducted two scTagger runs: one to extract the first 16 bp after the adapter match for barcode matching, and another to extract 28 bp after the match, considering the last 12 bases as UMIs for subsequent UMI correction. The detected barcodes and extracted UMI regions were tabulated, and unprocessed reads were used in downstream correction pipeline steps. To correct amplification errors, we utilized directional UMI clustering with the UMI-Tools suite.