MPC-2 and U2OS cells were lysed in RLY buffer (Bioline, Inc.). Total RNA from lysates was prepared using the Isolate II RNA Mini Kit (Bioline, Inc.). RNA samples were tested with intron-spanning primers/probe specific for mouse or human Fibroblast growth factor-23 ( Fgf23 ) and Transferrin receptor ( Tfrc ) and mouse Phd1 ( Egln2 ), Phd2 ( Egln1 ), Phd3 ( Egln3 ) mRNAs; Mouse or human Gapdh or β-actin was used as an internal control for RT-qPCR (Applied Biosystems/ThermoFisher Scientific). The TaqMan One-Step RT-PCR kit was used to perform the qPCR reactions under cycling conditions: 30 min 48 °C, 10 min 95 °C, followed by 40 cycles of 15 s 95 °C and 1 min 60 °C.